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Acellular dermal matrix-based hydrogel and preparation method thereof

An acellular dermis, hydrogel technology, applied in medical science, prosthesis and other directions, can solve problems such as affecting clinical use, increasing costs, environmental pollution, etc., and achieves the goal of supporting cell growth, reducing preparation costs, and low genetics. toxic effects

Active Publication Date: 2019-05-31
上海仁康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology describes how we use animal skins or cell culture materials called Acellecumina®(a brand name from L'Ecole Normale), create hydrogels made up mostly of collagen fibers without damaging their structures during processing steps like decellanolification. These hydrated gel matrices were found to reduce inflammability when placed inside living organisms due to its reduced DNA damage caused by bacteria. They could potentially replace synthetic polymeric substances commonly used in prostheses such as silicon rubber tubings.

Problems solved by technology

This patented technical solution described in this patent involves developing a new type of biomaterial called Acellecarnia collagen or ACL, which can be easily obtained from animal body fluids without causing harmful side effects during medical procedures. These materials also exhibit excellent strength, flexibility, durability, cell adhesion ability, and reduced inflammations compared to existing synthetic ones due to their chemical composition and structural features. Additionally, they may help healing damaged areas better than traditional treatments while reducing costs associated with surgery and recovery processes.

Method used

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  • Acellular dermal matrix-based hydrogel and preparation method thereof
  • Acellular dermal matrix-based hydrogel and preparation method thereof
  • Acellular dermal matrix-based hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A preparation method of human-derived acellular dermal matrix, the specific steps are as follows:

[0047] (1) Preparation of acellular dermal matrix:

[0048] ①Take the stored human skin and remove the subcutaneous fatty components;

[0049] ②Step ①Clean and disinfect the skin with subcutaneous fatty components with povidone iodine and alcohol, and then soak and clean the skin repeatedly with hypotonic solution;

[0050] ③ Treat the skin washed in step ② with 2.5 mg / ml Dispase II solution overnight; discard the Dispase II solution, remove the epidermis, and then wash with normal saline to obtain the dermis;

[0051] ④Use the dermis obtained in step ③ with 2mol / L Ca 2+ NaCl solution for 18h, Ca 2+ The final concentration is 5mM / L; after discarding the sodium chloride solution, wash with normal saline;

[0052] ⑤ Place the dermis cleaned in step ④ into the prepared "decellularized solution", which contains the following components: 0.1%-0.5wt% trypsin, 0.5-5mM EDTA, ...

Embodiment 2

[0056] Internal structure of acellular dermal matrix

[0057] (1) Cut the acellular dermal matrix obtained above into a size of 1 cm × 1 cm, and paste it on a metal tray; the section of the acellular dermal matrix is ​​quenched with liquid nitrogen to make it hard and brittle, and then broken; the section faces Paste on the metal tray.

[0058] (2) After spraying gold in the sample preparation room of the electron microscope, it was put on the machine, observed and photographed by the scanning electron microscope, and the results were as follows: image 3 shown.

[0059] The results showed that there were no cell residues in the tissue, and the collagen structure was complete, similar to normal skin.

Embodiment 3

[0060] Example 3 HE staining of acellular dermal matrix

[0061] (1) Fix the normal skin tissue and the acellular dermal matrix in 4% paraformaldehyde fixative solution for 24 hours respectively;

[0062] (2) Alcohol dehydration, xylene transparency;

[0063] (3) Dip in wax for 30 minutes, embed, and make sagittal paraffin sections with a thickness of 5 μm;

[0064] (4) Gradient alcohol carries out dewaxing, dehydration, HE staining;

[0065] (5) Transparent, sealed, observed under a light microscope, the results of HE staining of normal skin tissue are as follows: Figure 4 As shown, the results of HE staining of acellular dermal matrix are as follows Figure 5 shown.

[0066] The results showed that there were no cell residues in the skin tissue.

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Abstract

The invention discloses an acellular dermal matrix-based hydrogel and a preparation method thereof. The hydrogel uses an acellular dermal matrix as a raw material and crosslinks by using Ca2+ and EDC/NHS. The hydrogel has a porous structure, and the pores are communicated with each other. The hydrogel has a three-dimensional structure similar to human skin tissues, and enables the body tissue cells to grow rapidly. The hydrogel contains rich growth factors, has good biocompatibility and is more conducive to growth of supporting cells. The hydrogel has good shapeability, and can be prepared into different specifications according to needs. The hydrogel can not only be used for wound repair, but also is an excellent tissue engineered skin scaffold material, and can be used for the repair ofskin defects and preparation of tissue engineered skin.

Description

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Claims

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Application Information

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Owner 上海仁康科技有限公司
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