Preparation method of decellularized derma matrix of human source

Abstract

The invention discloses a preparation method of a decellularized derma matrix of a human source. The preparation method specifically comprises the following steps: taking stored human source skin; eliminating subcutaneous attached fat ingredients; cleaning and disinfecting by using iodine and ethyl alcohol, after that, repeatedly soaking and washing the skin by using a hypotonic solution; treatingby using a Dispase II solution overnight; removing epidermis, and after that, washing by using normal saline, thus acquiring derma; treating by using a hypertonic saline solution; washing by using the normal saline; treating by using a decellularizion solution in a dialysis device, thus acquiring the decellularized derma matrix; washing by using the normal saline, to obtain the decellularized derma matrix of the human source. The preparation method is simple and convenient, adopts a simple and reliable production process, is capable of improving production efficiency, reducing production cost, and reducing environment pollution in the greatest degree; in addition, the obtained decellularized derma matrix reserves a skin basement membrane, and is beneficial to adhesion and multiplication of cells; at the same time, the decellularized derma contains abundant growth factors, so the decellularized derma matrix has good biocompatibility, and is more beneficial to supporting growth of the cells.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a method for preparing human-derived acellular dermal matrix. Background technique [0002] Dermal substitutes play an important role in the wound healing process. They can provide a dermal scaffold at the bottom of the wound, improve the survival rate of the cultured epidermal membrane, and reduce wound shrinkage and scar formation. Skin grafts are the best treatment for patients with extensive burns or trauma. Acellular dermal matrix (ADM) has an internal structure similar to that of skin dermis, and has been used as a dermis substitute in both domestic and foreign experiments and clinics. [0003] Currently, there are two main types of acellular dermal matrix: allogeneic acellular dermal matrix and heterogeneous acellular dermal matrix. Compared with the heterogeneous acellular dermal matrix, practice and clinical use have confirmed that the allogeneic acellular de...

AI Technical Summary

Problems solved by technology

Although there are many preparation methods of acellular dermal matrix, there are more or less certain problems: the current preparation methods of acellular dermal matrix are very complicated, need enzyme digestion, lye treatment, etc., the steps are complicated and the process is complicated, which greatly increases the cost. Production cost; most of the current preparation methods of acellular dermal matrix use chemical fixatives, such as glutaraldehyde, which greatly changes the natural structure of acellular dermal matrix and hinders the acellular dermal matrix in vivo Degradation; At the same time, glutaraldehyde residues cause greater cytotoxicity and genotoxicity of decellularized products, which greatly affects clinical use; At present, there are also many acellular dermal matrix preparation methods that introduce alkaline solution and organic solvent treatment, which not only affects the decellularized products. The structure of the dermal matrix also increases the possibility of environmental pollution, and the treatment of waste liquid also increases the cost; such as repeated freezing and thawing, this method belongs to the use of physical methods for decellularization, which takes a long time and a long cycle. no longer needed

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