A kind of preparation method of human-derived acellular dermal matrix

Abstract

The invention discloses a preparation method of a human-sourced acellular dermal matrix. The specific steps are as follows: take the stored human-sourced skin; remove subcutaneous fat components; clean and disinfect with iodophor and alcohol, and then repeatedly soak and clean with a hypotonic solution Skin; treated overnight with Dispase II solution; removed epidermis, and then washed with normal saline to obtain dermis; treated with hypertonic saline solution; washed with normal saline; treated with decellularized solution in a dialysis device to obtain decellularized dermal matrix; Wash with normal saline to obtain human-derived acellular dermal matrix. The preparation method of the present invention is simple, the production process is simple and reliable, the production efficiency is improved, the production cost is saved, and the environmental pollution can be reduced to the greatest extent. At the same time, the obtained acellular dermal matrix retains the skin basement membrane, which is more conducive to cell adhesion and proliferation. Rich in growth factors, it has good biocompatibility and is more conducive to supporting the growth of cells.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a method for preparing human-derived acellular dermal matrix. Background technique [0002] Dermal substitutes play an important role in the wound healing process. They can provide a dermal scaffold at the bottom of the wound, improve the survival rate of the cultured epidermal membrane, and reduce wound shrinkage and scar formation. Skin grafts are the best treatment for patients with extensive burns or trauma. Acellular dermal matrix (ADM) has an internal structure similar to that of skin dermis, and has been used as a dermis substitute in both domestic and foreign experiments and clinics. [0003] Currently, there are two main types of acellular dermal matrix: allogeneic acellular dermal matrix and heterogeneous acellular dermal matrix. Compared with the heterogeneous acellular dermal matrix, practice and clinical use have confirmed that the allogeneic acellular de...

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Problems solved by technology

Although there are many preparation methods of acellular dermal matrix, there are more or less certain problems: the current preparation methods of acellular dermal matrix are very complicated, need enzyme digestion, lye treatment, etc., the steps are complicated and the process is complicated, which greatly increases the cost. Production cost; most of the current preparation methods of acellular dermal matrix use chemical fixatives, such as glutaraldehyde, which greatly changes the natural structure of acellular dermal matrix and hinders the acellular dermal matrix in vivo Degradation; At the same time, glutaraldehyde residues cause greater cytotoxicity and genotoxicity of decellularized products, which greatly affects clinical use; At present, there are also many acellular dermal matrix preparation methods that introduce alkaline solution and organic solvent treatment, which not only affects the decellularized products. The structure of the dermal matrix also increases the possibility of environmental pollution, and the treatment of waste liquid also increases the cost; such as repeated freezing and thawing, this method belongs to the use of physical methods for decellularization, which takes a long time and a long cycle. no longer needed

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