Vibrio alginolyticus selectivity differential medium

Abstract

The invention discloses a selectivity differential medium-vibrio alginolyticus selectivity differential medium (VaDM) which uses rapid screened vibrio alginolyticus as a unique target bacterium. The vibrio alginolyticus selectivity differential medium is prepared from leucine, yeast leaching powder, sodium chloride, sodium thiosulfate, sodium cholate, bile powder, ferric citrate, calcium chloride, agar, bromothymol blue, thymol blue and distilled water. In the invention, all components of the VaDM are optimized and combined according to a special leucine aminoase system of vibrio alginolyticus, basic and special nutrition requirement, halophilism and resistance on some bacteriostats, favorable growth of the bacterium is guaranteed, the growth of a plurality of other miscellaneous bacteriacan be effectively inhibited, the target bacterium presents special bacterial colony color and achieves good selecting and distinguishing effects with other bacteria. The invention verifies that VaDMhas an ideal effect on the rapid screening of vibrio alginolyticus by testing bacterium yield, accuracy, precision and sensitivity and comparing the conventional detecting method for detecting a practical sample.

Description

Technical field [0001] The invention relates to a microbial selective identification medium, which belongs to the field of food hygiene microbial safety detection. Background technique [0002] Food-borne diseases caused by ingestion of unsafe food are a common and increasingly serious public health problem in both developed and developing countries. Pathogenic Vibrio is one of the most important food-borne pathogens. It is the main pathogen that pollutes aquatic products, especially in coastal areas in the hot summer and autumn. It is the primary pathogen of food poisoning and can cause aquatic products. The disease, harm and loss are huge. [0003] Vibrio alginolyticus (Vibrio alginolyticus) is a gram-negative Vibrio with a single flagellum and is dynamic. It is widely distributed in drinking water, seawater and aquatic products on the ground, and its number ranks first among marine Vibrio species. The pollution rate is nearly 100 times higher than that of Vibrio parahaemolytic...

AI Technical Summary

Problems solved by technology

Commonly used selective media mainly include halophilic bacteria selective agar, sodium thiosulfate-sodium citrate-bile salt-sucrose agar medium (TCBS), but these media can only combine halophilic bacteria with non-halophilic bacteria. Bacteria are separated, or Vibrio is separated from other bacteria and Vibrio is divided into two categories according to its ability to utilize sucrose. Neither can detect a certain type of Vibrio, let alone specific and rapid detection of lysobacteria. Vibrio alginolyticus, and there is no specific proprietary medium for the rapid detection of Vibrio alginolyticus at home and abroad

Rapid screening methods for Vibrio alginolyticus include PCR, API 20E, and VITEK. These methods have their own advantages, but the instruments and reagent consumables required by these methods are relatively expensive, or have high technical requirements. The popular application of departments and enterprises brings certain difficulties

[0005] As an important part of the rapid screening technology for pathogenic microorganisms, the selective identification medium has the advantages of low cost, strong specificity, high sensitivity, easy operation and easy visual identification of test results, etc., and is widely used in inspection and quarantine institutions at all levels At present, there are many commercialized selective identification media at home and abroad, such as Vibrio parahaemolyticus, Escherichia coli O157H7, Salmonella and other special selective identification media are widely used in the rapid screening of pathogenic microorganisms However, there is no selective differential medium specifically for the rapid screening of Vibrio alginolyticus, so research and development on it are urgently needed

Images