Gene chip for detecting nine pathogenicity vibrios in marine products

Abstract

The invention relates to a gene chip for detecting nine pathogenicity vibrios in marine products and a kit thereof. The gene chip comprises a solid phase carrier and an oligonucleotide probe, wherein the oligonucleotide probe includes one or more selected from the following nucleotide sequences: 1) DNA sequences selected from genes of a vibrio hollisae, a vibrio vulnificus, a vibrio cholera, a vibrio parahemolyticus, a vibrio harveyi, a vibrio alginolyticus, a vibrio furnissi, a vibrio mimicus and a vibrio damsel, 2) complementary DNA sequences of the DNA sequences selected in the DNA sequences in 1), 3) complementary RNA sequences of the selected DNA sequences in 1) or 2). The kit comprises the gene chips. The gene chip for detecting nine pathogenicity vibrios in marine products and the kit thereof provided by the invention are used for detecting the nine pathogenicity vibrios in marine products, and have the advantages of simplicity in operation, good sensitivity and strong repeatability.

Description

technical field [0001] The invention belongs to the technical field of microbial molecular detection, and in particular relates to a gene chip for detecting nine kinds of pathogenic Vibrio in seafood. Background technique [0002] Zhejiang Province is a large ocean province with a rich variety of seafood. Seafood products are deeply loved by local people, and many residents have the habit of eating raw seafood. However, with the increasing pollution of the marine environment, pathogenic microorganisms are enriched in large quantities in aquatic products, and food-borne diseases and food poisoning caused by eating marine products have occurred frequently, posing a great threat to people's life and health. . Pathogenic Vibrio is the most important pathogenic microorganism in seafood, including Vibrio cholerae, Vibrio mimicus, parahaemolyticus, Hollisia, Vibrio alginolyticus, Furnaceium, Vibrio vulnificus, mermaid There are nine kinds of Vibrio and Vibrio harveylius. The path...

AI Technical Summary

Problems solved by technology

[0003] At present, the detection and identification methods of pathogenic bacteria in seafood in my country still remain at the routine microbiological inspection level, usually based on isolation culture, biochemical tests and serological tests, which have complex operations, heavy manual labor, and long inspection cycles (6-7 days). Days), low specificity and other shortcomings, to a considerable extent limit the ability of rapid detection and identification of food-borne disease pathogenic seafood

And when judging whether it is positive or not, it only depends on the naked eye observation of the experimenter, and there is not enough reliable basis, which reduces the accuracy of the experimental results, and cannot meet the requirements of short storage time and rapid detection of fresh seafood.

Some simple molecular biology methods such as PCR and ELISA have high false positive results, and the results obtained by using PCR and ELISA cannot be used as the final detection basis

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