41 results about "Vibrio splendidus" patented technology

The invention discloses a vibrio-splendidus-resistant egg yolk antibody and a preparation method thereof. The vibrio-splendidus-resistant egg yolk antibody is obtained from a vibrio splendidus immunized laying hens, has features of good specificity and high titer, is used for preventing and treating diseases caused by vibrio splendidus and immunological detection, and has good effects. The vibrio-splendidus-resistant egg yolk antibody is obtained by extracting hyperimmune eggs laid by the vibrio splendidus immunized laying hens, blood sampling is not required, immunized animals are not injured, and modern animal rights protection rules are met; and each egg contains more than 100mg of the vibrio-splendidus-resistant egg yolk antibody approximately, and 3g of the vibrio-splendidus-resistant egg yolk antibody can be obtained in a month, and is 10-20 times greater than an antibody extracted from blood of an immunized rabbit. The vibrio-splendidus-resistant egg yolk antibody has the advantages of safety, environmental protection, high efficiency, low cost and the like, and is easy to industrialize.

The invention relates to a scallop protein H2A gene clone and the monoclonal expression technique of N end antibiotic polypeptide in the field of molecular biology technique. It uses isogenesis clone technique to obtain the scallop group protein H2A gene group total length; the gene DNA has a 375bp open reading frame with the coding 125 amino acids, 3' non-coding area comprises two ending signals which are stem ring structure and polyadenylic acid rear signal. It uses pPIC9K expressing carrier to express the protein N end 30aa in Pichia pastoris, the expressing product has bacterial inhibition to the Micrococcus luteus and the Vibrio splendidus.

The invention relates to the technical field of detection of pathogenic bacteria of cultured sea cucumber skin disease syndromes, and particularly relates to a primer for rapidly detecting pathogenic bacteria, namely vibrio splendidus of the sea cucumber skin disease. The invention is used for overcoming the defects of the technology for detecting main pathogenic bacteria, namely vibrio splendidus of the sea cucumber skin disease syndromes of the existing sea cucumber, and providing a method for rapidly detecting pathogenic bacteria, namely vibrio splendidus of the sea cucumber skin disease syndrome. The method comprises the following steps: centrifuging, cleaning, freezing and thawing and boiling a pure bacterial culture as a template to carry out LAMP (loop-mediated isothermal amplification); designing an LAMP primer aiming at a vibrio splendidus flgF gene; configuring and optimizing a reaction system; bathing for 45 minutes at the temperature of 65 DEG C, and judging the result by naked eyes or electrophoresis. By adopting the method, the LAMP technology is firstly applied to detection of pathogenic bacteria, namely vibrio splendidus of the sea cucumber skin disease syndromes. The method has the advantages of being short in time consumption, low in cost, simple, convenient and easy to use, suitable for popularization in a basic level, and the like.

The invention relates to the technical field of detection of pathogenic bacteria of cultured sea cucumber skin disease syndromes, and particularly relates to a primer for rapidly detecting pathogenic bacteria, namely vibrio splendidus of the sea cucumber skin disease. The invention is used for overcoming the defects of the technology for detecting main pathogenic bacteria, namely vibrio splendidus of the sea cucumber skin disease syndromes of the existing sea cucumber, and providing a method for rapidly detecting pathogenic bacteria, namely vibrio splendidus of the sea cucumber skin disease syndrome. The method comprises the following steps: centrifuging, cleaning, freezing and thawing and boiling a pure bacterial culture as a template to carry out LAMP (loop-mediated isothermal amplification); designing an LAMP primer aiming at a vibrio splendidus flgF gene; configuring and optimizing a reaction system; bathing for 45 minutes at the temperature of 65 DEG C, and judging the result by naked eyes or electrophoresis. By adopting the method, the LAMP technology is firstly applied to detection of pathogenic bacteria, namely vibrio splendidus of the sea cucumber skin disease syndromes. The method has the advantages of being short in time consumption, low in cost, simple, convenient and easy to use, suitable for popularization in a basic level, and the like.

The invention discloses a genetic molecular marking combination for anti-disease stichopus japonicas breeding and application of the genetic molecular marking combination and belongs to the field of aquatic product genetic breeding. The genetic molecular marking combination consists of three SNP (Single Nucleotide Polymorphism) markers SNP88, SNP112 and SNP126 related to anti-vibrio splendidus infection of stichopus japonicas, and the three marked dominant genotypes are respectively SNP88 (CC), SNP112 (AA) and SNP126 (TT). A breeding method with the genetic markers comprises the following operation steps: (1) extracting parent DNA (Deoxyribonucleic Acid) and typing; (2) selecting parents; (3) performing filial generation seedling breeding. By adopting the genetic molecular marking combination, effective molecular markers for improving the economic property of anti-vibrio splendidus infection of stichopus japonicas are provided, the genetic markers are adopted for auxiliary marker selection of stichopus japonicas, anti-disease breeding can be carried out, the anti-vibrio splendidus infection of the stichopus japonicas can be improved, the morbidity of skin ulcer syndromes of the stichopus japonicas can be substantially reduced, and the combination has great significances in increasing the breeding survival rate and the breeding benefits.

The invention relates to a scallop protein H2A gene clone and the monoclonal expression technique of N end antibiotic polypeptide in the field of molecular biology technique. It uses isogenesis clone technique to obtain the scallop group protein H2A gene group total length; the gene DNA has a 375bp open reading frame with the coding 125 amino acids, 3' non-coding area comprises two ending signals which are stem ring structure and polyadenylic acid rear signal. It uses pPIC9K expressing carrier to express the protein N end 30aa in Pichia pastoris, the expressing product has bacterial inhibition to the Micrococcus luteus and the Vibrio splendidus.

The invention relates to a microecological preparation used for preventing and treating apostichopus japonicus putrid skin syndrome and a preparation method of the microecological preparation and belongs to the field of aquatic microorganisms. Active ingredients of the microecological preparation are thalli of bacillus megatherium H1 and extracellular metabolites of the bacillus megatherium H1; the bacillus megatherium strain is separated from intestinal tract excrement of apostichopus japonicus which is healthily cultivated in Penglai, Shandong, detection of safety, bacteriostatic activity and toxicity and artificial antagonistic vibrio splendidus experiments verify that the bacillus megatherium H1 is antagonistic to vibrio splendidus, young apostichopus japonicus is effectively protected, the bacillus megatherium H1 can be used for preventing and treating apostichopus japonicus 'putrid skin syndrome' caused by vibrio splendidus and survival rate of an apostichopus japonicus seedling protecting stage is increased.

The invention discloses a gene chip detecting marine pathogenic vibrios, and a preparation method and a detection method thereof. The gene chip comprises a solid-phase carrier and detection probes fixedly disposed on the solid-phase carrier, and the detection probes comprise vibrio vulnificus gyrB gene probe, vibrio vulnificus virulence gene hemA probe, vibrio splendidus gyrB gene probe, vibrio splendidus virulence gene toxR probe, vibrio harveyi gyrB gene probe, vibrio harveyi virulence gene hemA probe, vibrio parahaemolyticus gyrB gene probe, vibrio parahaemolyticus virulence gene toxS probe, vibrio anguillarum gyrB gene probe and vibrio anguillarum virulence gene hem probe which are respectively shown as SEQ NO 1-10. The gene chip is firstly applied to detection on marine pathogenic vibrios. The gene chip is capable of rapidly sensitively detecting target bacterium infectio, also is good in repeatability and strong in signal, and does not easily cause a nonspecific signal.

The invention belongs to the technical field of aquatic organisms and in particular relates to a quick detection method for scallop pathogenic vibrio splendidus. Total DNA (deoxyribonucleic acid) of bacterial genomes in a filtered seawater sample to be detected is taken as a template, fluorescent quantitative PCR (polymerase chain reaction) amplification is carried out by a bacterial 16SrDNA conserved region and a specific primer of a pathogenic vibrio splendidus metalloproteinases gene, and the scallop pathogenic vibrio splendidus in the culture environment is quantitatively analyzed and detected; the detection method comprises the steps of extracting the DNA of the bacterial genomes and carrying out fluorescent quantitative PCR detection. The method has the advantages that the quickness is achieved, the specificity is good, the bacterial density detection range is wide, and a quantitative result can be close to a real situation.

The invention discloses a feed additive for stichopus japonicus, a preparation method and a compound feed containing the feed additive. The feed additive for the stichopus japonicus comprises the following raw materials: rhubarb, baical skullcap root, Chinese gall, honeysuckle flower, wild chrysanthemum, indigowoad root and a synergistic agent. The preparation method comprises the following steps of: weighing the raw materials in a weight ratio; crushing all the raw materials; and putting all the crushed raw materials into a mixer sequentially, and mixing uniformly. The compound feed containing the additive comprises 1 to 3 weight percent of additive. The technical scheme provided by the embodiment of the invention has the advantages that: the additive is prepared from Chinese medicinal herbs, does not have medicinal tolerance and toxic or side effects, can improve the growth speed of the stichopus japonicus obviously, improve the immunological activity of the stichopus japonicus and enhance the disease-resistant capacity of the stichopus japonicus, and has extremely high resistance to vibrio splendidus serving as main pathogenic bacteria. The stichopus japonicus is difficult to sicken, the survival rate is improved obviously, and the production benefit is improved obviously.

The invention discloses a method for quickly detecting pathogen of skin ulcerative syndrome in Apostichopus on site and a kit thereof. In the method, two pairs of primers, particular operation steps and the kit aiming at the vibrio splendidus are designed by using a loop-mediated isothermal amplification (LAMP) method. The DNA sequences of the reaction primer are as follows: VS-F3 is 5'-TGGTCCCCCCATATTCAGAC; VS-B3 is 5'-AAGTACCCCGAGGAAGAGAA; VS-FIP is 5'-GCCCCGTAACCGACACATCATTTTAGGATATCACGTGTCCCGC; and VS-BIP is 5'-GGCACTTTCCAGAGCCTTCGTTTTTAGATTCCGAAAGTAGCGGC. By using the method, expensive instrument and equipment or long detecting time is not required, the pathogen can be detected even less pathogen exists. The method has the advantages of low cost, accurate detection and the like, is convenient to operate, real-time monitor can be performed, and the epidemic situation can be discovered in time, the incidence trend of the epidemic disease is reduced, the pathogen can be prevented from distributing in early stage, and more loss brought to raisers is avoided.

The invention relates to a gold-labelled nucleic acid test strip for quickly detecting aquatic diseases. The gold-labelled nucleic acid test strip comprises a sample pad, a gold-labelled pad containing probe Probe1-AuNPs composite, a nitrocellulose membrane and a water absorbent pad; a detection line and a quality control line which are separated from each other are respectively adsorbed on the nitrocellulose membrane; the detection line is composed of a probe2, and the quality control line is composed of the probe 1'; the probe1 and the probe2 are respectively designed by selecting a characteristic conserved segment from a vibrio splendidus toxicity gene sequence and using the 5' end and 3' end of the characteristic conserved segment, and then a complementary sequence Probe1' is designedaccording to the probe1. A nano-gold nucleic acid probe is used as the label in the development of the gold-labelled nucleic acid test strip, and the vibrio splendidus is detected through a thin-filmchromatography measure, thereby providing simple, convenient and efficient detection method for the disease control of the vibrio splendidus.

The invention discloses a vibrio-splendidus-resistant egg yolk antibody and a preparation method thereof. The vibrio-splendidus-resistant egg yolk antibody is obtained from a vibrio splendidus immunized laying hens, has features of good specificity and high titer, is used for preventing and treating diseases caused by vibrio splendidus and immunological detection, and has good effects. The vibrio-splendidus-resistant egg yolk antibody is obtained by extracting hyperimmune eggs laid by the vibrio splendidus immunized laying hens, blood sampling is not required, immunized animals are not injured, and modern animal rights protection rules are met; and each egg contains more than 100mg of the vibrio-splendidus-resistant egg yolk antibody approximately, and 3g of the vibrio-splendidus-resistant egg yolk antibody can be obtained in a month, and is 10-20 times greater than an antibody extracted from blood of an immunized rabbit. The vibrio-splendidus-resistant egg yolk antibody has the advantages of safety, environmental protection, high efficiency, low cost and the like, and is easy to industrialize.