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Gene chip for detecting ten types of pathogenic bacteria in sea areas

A technology of gene chip and pathogenic bacteria, which is applied in the field of gene chip for detecting 10 kinds of pathogenic bacteria in sea area, can solve the problems of human health threat, affecting the marine function of coastal waters, affecting human's development and utilization of the ocean, etc. The effect of low sample consumption

Inactive Publication Date: 2014-01-29
NINGBO UNIV
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Problems solved by technology

[0002] With the intensification of human development of the ocean, the pressure on the ecological environment of the sea area is also increasing day by day, and the existence of pathogenic microorganisms in the marine environment and the mass reproduction under suitable conditions have affected human development and utilization of the ocean. The marine function of the coastal waters is compromised, and it also poses a potential threat to human health

Method used

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  • Gene chip for detecting ten types of pathogenic bacteria in sea areas
  • Gene chip for detecting ten types of pathogenic bacteria in sea areas
  • Gene chip for detecting ten types of pathogenic bacteria in sea areas

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Embodiment Construction

[0007] Below in conjunction with embodiment the present invention is described in further detail.

[0008] 1. Selection of solid phase carrier (substrate)

[0009] Commonly used solid phase supports include nylon membranes, nitrocellulose membranes, glass flakes, and films made of various organic polymers. The glass flakes used in this example are 75.5mm×25.2mm×1.0mm as substrates. The appearance has no scratches and no defects. The surface of the glass sheet must be chemically modified before the DNA probe can be fixed on the substrate. The existing chemical modifications on the surface of the glass sheet mainly include amino modification, aldehyde group modification and sulfhydryl group modification. In this example, glass flakes are selected to be modified with aldehyde groups, which is a product of Beijing Boao Biological Co., Ltd.

[0010] 2. Preparation of probes

[0011] 1. Design of quality control probes: The quality control probes shown in Table 1 were used in the ...

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Abstract

The invention discloses a gene chip for detecting ten types of pathogenic bacteria in sea areas. The gene chip comprises a solid phase slide on which a quality control probe and a detection probe are arranged, wherein the nucleotide sequence components of the detection probe are shown in SEQ ID NO.1-SEQ ID NO.27. The gene chip is capable of simultaneously detecting enterobacter cloacae, edwardsiella tarda, streptococcus faecalis, vibrio fortis, vibrio harveyi, vibrio parahaemolyticus, vibrio splendidus, vibrio vulnificus, vibrio anguillarum and shewanella smarisflavi just in the presence of DNA (deoxyribose Nucleic Acid) without need of live bacteria. The gene chip has remarkable advantages of high throughput, parallelism, miniaturization, automation, rapidness, sensitiveness, quantification, small use amount, and the like.

Description

technical field [0001] The invention relates to a bacterial gene chip, in particular to a gene chip for detecting 10 kinds of pathogenic bacteria in sea areas. Background technique [0002] With the intensification of human development of the ocean, the pressure on the ecological environment of the sea area is also increasing day by day, and the existence of pathogenic microorganisms in the marine environment and the mass reproduction under suitable conditions have affected human development and utilization of the ocean. The marine function of the coastal waters is compromised, and it also poses a potential threat to human health. First of all, for example, human and zoonotic pathogens infect humans through eating seafood, which seriously endangers human health and life safety. Incidents such as cholera and diarrhea caused by Vibrio cholerae and Vibrio parahaemolyticus in seafood are frequent. send. Secondly, bacterial diseases spread due to leisure and entertainment activ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/10C12Q1/04
CPCC12Q1/04C12Q1/6837C12Q2565/501Y02A50/30
Inventor 苏秀榕周君李太武
Owner NINGBO UNIV
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