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The invention relates to an intelligent force control robot grinding system. The intelligent force control robot grinding system comprises a computer, an industrial camera, a robot, a robot controller, a calibration and compensation system, a safety protection unit, a force sensor and an automatic knife changing system. The computer is connected with the industrial camera. The robot controller is connected with the computer, the robot, the calibration and compensation system, the safety protection unit, the force sensor and the automatic knife changing system. A method comprises the steps that the computer receives workpiece scanning data collected by the industrial camera, generates a knife path and converts the knife path into the robot posture and the tail end path; calibration and compensation are carried out; the robot controller controls all joints to move while the robot posture and the tail end path are adjusted. Hardware of a high-precision force control grinding system is achieved, the path self-calibration and online compensation technology is adopted, and the machining of a complex hook face with the high requirements for machining precision and surface smoothness can be finished.

Methods of oxidizing multiwalled carbon nanotubes are provided. The multiwalled carbon nanotubes are oxidized by contacting the carbon nanotubes with gas-phase oxidizing agents such as CO2, O2, steam, N2O, NO, NO2, O3, and ClO2. Near critical and supercritical water can also be used as oxidizing agents. The multiwalled carbon nanotubes oxidized according to methods of the invention can be used to prepare rigid porous structures which can be utilized to form electrodes for fabrication of improved electrochemical capacitors.

Methods are disclosed for the capture, detection, separation, isolation and quantification of contaminants in a starting material. Also disclosed are competitive assay methods for the detection and quantification of contaminants in a starting material. Kits for use with the method are disclosed as well. A system for capturing, separating and / or concentrating contaminants from a material is also presented. The system captures, separates and / or concentrates contaminants such as bacteria, viruses, other microorganisms, and / or larger items, such as insects, from a variety of materials, such as food, and environmental and clinical materials. In general, the system uses a rotating magnetic field to mix the material with magnetic particles to capture the target contaminants, and a fixed magnetic field to separate and concentrate the captured target contaminants.

An electrical energy storage device for storing electrical energy and supplying the electrical energy to a driving motor at different power levels is disclosed. The electrical storage device has an energy battery connected to a power battery. The energy battery has a higher energy density than the power battery. However, the power battery can provide electrical power to the electrical motor at different power rates, thereby ensuring that the motor has sufficient power and current when needed. The power battery can be recharged by the energy storage battery. In this way, the power battery temporarily stores electrical energy received from the energy battery and both batteries can provide electrical energy at the different power rates as required by the motor. The energy storage device can be releasably connected to an external power source in order to recharge both batteries. Both batteries can be recharged independently to optimize the recharging and lifetime characteristics of the batteries.

Methods are provided for detecting, separating, isolating and quantifying contaminants in starting materials by separating the contaminant from the starting material using a bead coupled to an affinity moiety and quantum dot-labeling the contaminant. The contaminant is detected by the characteristic emission spectrum of the quantum dot. Also, competitive binding methods are provided wherein the starting material and a control material are contacted with a quantum dot coupled to an affinity moiety capable of binding the contaminant and a competitor complex. A decrease in the intensity of the characteristic emission spectrum of the quantum dot associated with the competitor complex from the starting material as compared to that of the control material is indicative of the presence of the contaminant in the starting material.

Disclosed are methods and apparatus for automatic visual detection of events, for recording images of those events and retrieving them for display and human or automated analysis, and for sending synchronized signals to external equipment when events are detected. An event corresponds to a specific condition, among some time-varying conditions within the field of view of an imaging device, that can be detected by visual means based on capturing and analyzing digital images of a two-dimensional field of view in which the event may occur. Events may correspond to rare, short duration mechanical failures for which obtaining images for analysis is desirable. Events are detected by considering evidence obtained from an analysis of multiple images of the field of view, during which time moving mechanical components can be seen from multiple viewing perspectives.

An electrical energy storage device for storing electrical energy and supplying the electrical energy to a driving motor at different power levels is disclosed. The electrical storage device has an energy battery connected to a power battery. The energy battery has a higher energy density than the power battery. However, the power battery can provide electrical power to the electrical motor at different power rates, thereby ensuring that the motor has sufficient power and current when needed. The power battery is continuously recharged by the energy storage battery. In this way, the power battery temporarily stores electrical energy received from the energy battery and provides the electrical energy at the different power rates as required by the motor. The energy storage device can be releasably connected to an external power source in order to recharge both batteries. Both batteries can be recharged independently to optimize the recharging and lifetime characteristics of the batteries.

The invention provides a personnel identification method and system combining video identification and UWB (ultra-wideband) positioning technologies. The method comprise the steps of: A, in the simulation filed of a same supervision area, obtaining geographical positions of mobile positioning objects and the first quantity of the geographical positions according to video identification positioning, and obtaining positioning coordinates of positioning terminals and the second quantity of the positioning coordinates according to wireless positioning; B, comparing the first quantity and the second quantity at a same time, prompting abnormity and entering C if the first quantity is not equal to the second quantity, and prompting normality if the first quantity is equal to the second quantity; and C, obtaining abnormal geographical positions according to the matching between the positioning coordinates and the geographical positions. The method and system can efficiently monitor specific objects and specific occasions such as a campus, analyze and determine in real time position monitoring and video image monitoring information, and timely provide alarm response service, thereby enhancing campus safety service.

Disclosed are methods and apparatus for automatic visual detection of events, for recording images of those events and retrieving them for display and human or automated analysis, and for sending synchronized signals to external equipment when events are detected. An event corresponds to a specific condition, among some time-varying conditions within the field of view of an imaging device, that can be detected by visual means based on capturing and analyzing digital images of a two-dimensional field of view in which the event may occur. Events may correspond to rare, short duration mechanical failures for which obtaining images for analysis is desirable. Events are detected by considering evidence obtained from an analysis of multiple images of the field of view, during which time moving mechanical components can be seen from multiple viewing perspectives.

Disclosed are methods and apparatus for automatic visual detection of events, for recording images of those events and retrieving them for display and human or automated analysis, and for sending synchronized signals to external equipment when events are detected. An event corresponds to a specific condition, among some time-varying conditions within the field of view of an imaging device, that can be detected by visual means based on capturing and analyzing digital images of a two-dimensional field of view in which the event may occur. Events may correspond to rare, short duration mechanical failures for which obtaining images for analysis is desirable. Events are detected by considering evidence obtained from an analysis of multiple images of the field of view, during which time moving mechanical components can be seen from multiple viewing perspectives.

Disclosed are methods and apparatus for automatic visual detection of events, for recording images of those events and retrieving them for display and human or automated analysis, and for sending synchronized signals to external equipment when events are detected. An event corresponds to a specific condition, among some time-varying conditions within the field of view of an imaging device, that can be detected by visual means based on capturing and analyzing digital images of a two-dimensional field of view in which the event may occur. Events may correspond to rare, short duration mechanical failures for which obtaining images for analysis is desirable. Events are detected by considering evidence obtained from an analysis of multiple images of the field of view, during which time moving mechanical components can be seen from multiple viewing perspectives.

There is provided a method for detecting a target RNA molecule in a sample. The method comprises reverse transcribing the target RNA contained in the sample using an RT oligonucleotide, the RT oligonucleotide comprising a stem-loop portion containing one or more nucleotides modified or modifiable to block DNA polymerase extension and a target annealing portion that is complementary to a downstream portion of the target RNA, the target annealing portion located 3′ to the stem-loop portion, to produce a reverse transcription product that comprises the RT oligonucleotide and a 3′ extended region; amplifying the reverse transcription product using (i) a first amplification primer that anneals to a downstream portion of the 3′ extended region of the reverse transcription product and (ii) a second amplification primer that anneals to an interface portion of a DNA strand complementary to the reverse transcription product, the interface portion comprising a region that is complementary to a 3′ portion of the RT oligonucleotide and a 5′ portion of the 3′ extended region in the reverse transcription product, to produce an amplification product; and detecting the amplification product; wherein the stem-loop portion adopts a stem-loop structure under conditions used for said reverse transcribing but does not adopt the stem-loop structure under conditions used for said amplifying and wherein when the stem-loop portion contains one or more nucleotides that are modifiable to block DNA polymerase extension, the method further comprises modifying the modifiable nucleotide prior to said amplifying.

An object of the present invention is to provide a polylactic acid composition having specific thermal characteristics and gas barrier properties, and to obtain a molding comprising a polylactic acid composition of PLLA and PDLA capable of forming a polylactic acid stretched film or other molding having superior surface smoothness, transparency, heat resistance and toughness, and the constitution thereof is characterized by being a molding comprising a polylactic acid composition in which the peak in DSC measurement during cooling (at a rate of 10° C. / min) after allowing to remain at 250° C. for 10 minutes is 30 mJ / mg or more, and preferably comprising a polylactic acid composition such that, in measurement during the DSC 2nd heating (cooling at a rate of 10° C. / min after allowing to remain at 250° C. for 10 minutes followed by reheating from 0° C. at a rate of 10° C. / min), the peak ratio (peak 1 / peak 2) of the peak when Tm is 150 to 180° C. (peak 1) to the peak when Tm is 200 to 240° C. (peak 2) is 0.5 or less; and a molding comprising a composition in which an inorganic filler is incorporated in the composition described above.

The invention relates to the technical field of chemical sensor, in particular to a phosphorescent chemical sensor for qualitatively detecting homocysteine and an application thereof. Aldehyde group of iridium complex can take cyclization with amido and hydrosulphonyl in the homocysteine to be transformed into hexahydroxy heterocycle and transformed into another compound. The original iridium complex is conjugated with a benzene ring and the aldehyde group and represents as weak red fluorescence while the compound obtained after reaction is not conjugated with the aldehyde group and represents as strong green fluorescence. Ultraviolet and phosphorescent spectrum is used for detecting the result that the complex identifies the homocysteine, thereby showing that the complex has specific response towards the homocysteine, has high sensitivity and can be identified by naked eye, and furthermore, the complex can distinguish cysteine which has a structure very similar as the homocysteine.

The invention relates to a nucleic acid aptamer electrochemical sensor based on metal organic framework materials (MOFs) as signal probes. The senor is used for detection of beta-amyloid (A beta) oligomers and belongs to the technical field of functional nanocomposite materials and biosensor detection. AuNFs-modified glassy carbon electrodes immobilize a first layer of aptamers, the A beta oligomer is further identified and through combination with second layer aptamer-modified gold nanoparticle-loaded Cu-MOFs (AuNPs / Cu-MOFs), the sandwich electrochemical sensor for detection of the A beta oligomer is formed. AuNPs / Cu-MOFs as a redox medium can directly detect a Cu<2+> signal and electrochemically active molecule labeling is avoided so that the detection processes are greatly simplified and the sensitivity of detection is improved.

The invention provides a device and a method for rapid quantitative detection of bacteria. The method comprises the following steps: the immune technique is adopted for marking bacteria in a sample with a fluorescent substance (such as FITC, fluorescence quantum dots, and the like); as a sample solution flows through a set of filtering device, the bacteria marked with the fluorescent substance settle on the surface of a filtering membrane; the fluorescent substance on the bacteria emits fluorescence by adopting laser to induce and arouse fluorescence; and the number of the bacteria in the sample can be obtained quickly and accurately by detecting the signal strength of all the fluorescence on the filtering membrane. The entire detecting process lasts for less than 30 minutes.

The invention which belongs to the medical field of genetic engineering and reproduction discloses a seminal plasma miRNA marker associated with human non-obstructive azoospermia and an application thereof. The maker is selected from several of hsa-miR-141, hsa-miR-193a-5p, hsa-miR-590-5p and hsa-miR-7-1*. The maker which has specificities and sensitivities to the non-obstructive azoospermia can be used for the preparation of a reagent for non-obstructive azoospermia diagnosis or monitoring, so invasive diagnosis is avoided, repeated detection is realized, and the dynamic monitoring of the obstacle degree of sperm generation can be easily achieved.

The invention discloses a fluorescence molecular probe and application thereof in hydrogen sulfide detection. The structure of the fluorescence molecular probe is R1-N3, wherein R1 is a gene with aggregated and induced luminescence property. The principle is as follows: the molecule containing a nitrine functional group does not shine in a solution or an aggregation state due to the quenching effect of nitrine while the molecule containing amino generated after reduction of the nitrine functional group by using hydrogen sulfide displays the characteristics of an adaptive image enhancer (AIE). Thus, detection of the hydrogen sulfide concentration in the aggregation state is achieved. The principle is as follows: the fluorescence quenching of the overall aggregation is caused by azide groups left in the aggregation state. Therefore, luminescence of the probe molecule just can be opened after most of azide groups are reduced. Thus, the detection limit and the effective detection range are regulated and controlled by regulating and controlling the concentration of the probe molecule.

The invention relates to a gene chip for typing influenza A virus, a preparation method thereof, a detection method and a detection kit, wherein the gene chip comprises a solid phase carrier and an oligonucleotide probe which is fixed on the solid phase carrier and contains DNA segments selected from one or more genes of HA, NA and / or M of the influenza A virus. The gene chip of the invention has very high sensitivity and specificity, can be used for simultaneously detecting a plurality of groups of influenza A virus genes and identifying groups by adopting a multiple PCR amplification mode, has wide application prospects, can identify the specific serotype, has efficient and quick detection and analysis methods, and can be applied to the fields of life science, medical testing, food safety, environmental surveillance, import and export inspection and quarantine, bioterrorism prevention, epidemiological surveillance, and the like.

The invention relates to a method for acquisition of an oligonucleotide probe. The method comprises the following steps: downloading genomic sequence of crop species from a public database, filtering and finding out tandem repeat sequences, screening preliminarily found repetitive sequences, comparing residual tandem repeat sequence and known probe sequence, removing already used probe sequence, comparing residual screened tandem repeat sequences, carrying out probe design and synthesizing and verifying the designed probe sequence so as to obtain effective oligonucleotide sequence. According to the invention, cost is low and efficiency is high. The probe can be used for non-degenerated fluorescence in situ hybridization (ND-FISH) analysis of crop chromosome, determination of distribution of tandem repeat sequences represented by the probe on chromosome, understanding of the structure characteristics of chromosome and establishment of specific landmark of chromosome. Thus, specific chromosome or chromosome region of crops is identified, and the developed new oligonucleotide probe has specificity of chromosome or chromosome region.

The invention discloses an FPGA (Field Programmable Gate Array)-based pulse wave signal generator comprising an LCD (Liquid Crystal Display), an oscilloscope, an FPGA, a touch screen, a power supply, an AD (Analog-to-Digital) conversion device and a DA (Digital-to-Analog) conversion device, wherein the input end of the DA conversion device is connected with the FPGA, and the output end of the DA conversion device is connected with the oscilloscope; the input end of the AD conversion device is connected with the FPGA, and the output end of the AD conversion device is connected with the touch screen; the LCD is connected with the FPGA; and the power supply is connected with the LCD. A waveform of a pulse wave is generated in two ways in a method provided by the invention, wherein one way is a Gaussian function and Cosine function combination way, the pulse wave is subjected to segmentation fitting to obtain the final complete waveform, the storage space wasted by the whole waveform is omitted, and the Gaussian function and Cosine function combination way has specificity; and the other way is as follows: an elastic cavity model is adopted for simulating the condition of a vessel to form two elastic cavities of the vessel, and waveforms of pulse waves under four conditions of low resistance, middle resistance, high resistance and ultrahigh resistance can be generated through changing parameters. The both ways can be set by a user on the premise that signal data is unknown, so that an accurate waveform of the pulse wave is obtained and output.

The invention relates to a surface modified carbon nano point, preparation thereof, application of the surface modified carbon nano point used as a fluorescence probe for detecting Cu2+ and glutathione. The invention relates to a detection method of the Cu2+ and the glutathione, and aims to solve the technical problems that an existing fluorescence probe for detecting the Cu2+ is easy to interfere by other metal ions, high in preparation cost and complex in preparation step. The triethylene tetramine-modified carbon nano point is used as the fluorescence probe for detecting the Cu2+ and the glutathione; the preparation method of the fluorescence probe comprises the steps of microwave heating citric acid and urea in a water solution, adding the acquired carbon nano point into an ethanol solution containing triethylene tetramine for reflowing, and preparing the fluorescence probe. The preparation method is simple, the reaction conditions are moderate, and the cost is low. A detection method comprises the steps of fluorescence quenching TCDs and forming a TCDs-Cu2+ compound through interaction of the fluorescence probe and the Cu2+, further interacting the TCDs-Cu2+ compound and the glutathione, recovering fluorescence of the TCDs, and detecting the Cu2+ and the glutathione. The probe can be used for detecting copper ions and the glutathione in the water solution and a life entity.

The invention provides an anti-spam method based on privacy protection, belonging to the computer network security field. The method is applicable to internet in which email address information needs to be protected. In the method, the frequently-used email address of a user is protected as privacy information without disclosure on the premise of guaranteeing smooth network interaction; and a sub-email address code with term of validity which can be flexibly controlled by the user replaces the frequently-used email address, and the frequently-used email address of the user is hidden as the privacy information, wherein, the sub-email address code is generated according to the email address, the system time and the random number of an interaction party and only used in the case of one-on-one communication between the specific interaction party and the user. The anti-spam method of the invention can keep the frequently-used email address of the user unpublished, emphasize autonomy of the user, and help the user independently judge and identify a spam and stop sending the spam from a source.

The invention relates to a natural antioxidant detecting method, which is characterized in that a KIO3-MA-H2O2-H2SO4-[NiL](ClO4)2 nonlinear oscillation system is used as detection liquid, a work curve is built for the oscillatory response of the solution on natural antioxidants, further, the qualitative or quantitative analysis on the natural antioxidants is realized, L in the [NiL](ClO4)2 is 5,7,7,12,14,14-hexamethyl-1,4,8,11-tetraazacyclotetradeca-4,11-diene, and all ingredients in the detection solution have the following mol concentrations that potassium iodate is 0.007 to 0.0735 mol / L, malonic acid is 0.025 to 0.375 mol / L, sulfuric acid is 0.00625 to 0.375 mol / L, hydrogen peroxide is 0.6 to 1.4 mol / L, and [NiL](ClO4)2 is 20.217 to 7.26 mol / L. The method has the advantages that the oscillation service life of the nonlinear system is long, the stability is high, specificity and high sensitivity are realized on the natural antioxidants, the quantitative analysis can be realized, meanwhile, the qualitative analysis can be carried out, in addition, the operation is simple, and convenience and high speed are realized.

This invention relates, e.g., to a method for determining if a subject has myocardial ischemia, comprising (a) providing a blood sample obtained from a subject suspected of having myocardial ischemia; (b) determining in the sample the amount of one or more of the following proteins: (i) Lumican and / or (ii) Extracellular matrix protein 1 and / or (iii) Carboxypeptidase N; and (c) comparing the amount(s) of the protein(s) to a baseline value that is indicative of the amount of the protein in a subject that does not have myocardial ischemia, wherein a statistically significantly increased amount of the protein(s) compared to the baseline value is indicative of myocardial ischemia. Other proteins indicative of myocardial ischemia are also described, as are methods for treating a subject based on a diagnostic procedure of the invention, and kits for carrying out a method of the invention.

The invention provides a method, device and system for writing personal data in a financial IC card. The method comprises the steps that an existing personal device is developed into a universal preset personal device, then a target configuration file is obtained according to target financial items and a target card platform, the preset personal device is configured through the specific target configuration file to obtain a target personal device, and due to the fact that the target configuration file is specially suitable for the target financial items and the target card platform, the target personal device configured and obtained through the target configuration file has specificity, and the acquired personal data are written in the financial IC card through the target personal device. According to the method, the personal device does not need to be repeatedly developed for the specific items and the card platform, the card writing efficiency of the financial IC card is improved, and the time and effort of developers are saved.