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Nucleic acid aptamer electrochemical sensor based on metal organic framework materials as signal probes

A metal-organic framework, nucleic acid aptamer technology, applied in the direction of electrochemical variables of materials, etc., to achieve the effect of improving sensitivity, simplifying detection steps, and promoting electron transfer

Active Publication Date: 2018-06-15
SHANGQIU NORMAL UNIVERSITY
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Problems solved by technology

The research group designed a sandwich-type immunoelectrochemical sensor of antibody-nucleic acid aptamer to detect Aβ oligomers, which has high sensitivity and specificity (Y.L.Zhou, H.Q.Zhang, L.T.Liu, C.M.Li, Z.Chang, X.Zhu, B.X.Ye, M.T.Xu, Scientific Reports, 2016, 6, 35186); however, antibodies are still used in this design, and the modification of nucleic acid aptamers to the surface of gold nanoparticles requires further labeling of electrochemically active molecules to introduce electrochemistry. chemical signal

Method used

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  • Nucleic acid aptamer electrochemical sensor based on metal organic framework materials as signal probes
  • Nucleic acid aptamer electrochemical sensor based on metal organic framework materials as signal probes
  • Nucleic acid aptamer electrochemical sensor based on metal organic framework materials as signal probes

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Embodiment 1

[0029] Example 1 Detection of the preparation method of a sandwich-type nucleic acid aptamer sensor based on Cu-MOFs as a signal probe

[0030] (1) Preparation of AuNPs / Cu-MOFs: 3.6mmol trimesic acid was dissolved in 12mL methanol under ultrasonic conditions, and 2.0mmol CuSO 4 ·3H 2 O dissolved in 12 mL H 2 O, then CuSO 4 ·3H 2 O solution was added dropwise into the above trimesic acid solution. Next, the mixture was allowed to stand at room temperature for 2 hours to produce a blue precipitate. Finally, the precipitate was repeatedly washed with water and methanol by centrifugation, and dried in vacuum overnight to obtain Cu-MOFs. Then, 50 mg of Cu-MOFs powder was added to 15 mL of AuNPs (15 nm) solution prepared by citrate reduction method and stirred continuously at room temperature for 48 h, resulting in a purple precipitate, AuNPs / Cu-MOFs, visible to the naked eye. Finally, the precipitate was washed repeatedly with ethanol and water, and dried in vacuum.

[0031]...

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Abstract

The invention relates to a nucleic acid aptamer electrochemical sensor based on metal organic framework materials (MOFs) as signal probes. The senor is used for detection of beta-amyloid (A beta) oligomers and belongs to the technical field of functional nanocomposite materials and biosensor detection. AuNFs-modified glassy carbon electrodes immobilize a first layer of aptamers, the A beta oligomer is further identified and through combination with second layer aptamer-modified gold nanoparticle-loaded Cu-MOFs (AuNPs / Cu-MOFs), the sandwich electrochemical sensor for detection of the A beta oligomer is formed. AuNPs / Cu-MOFs as a redox medium can directly detect a Cu<2+> signal and electrochemically active molecule labeling is avoided so that the detection processes are greatly simplified and the sensitivity of detection is improved.

Description

technical field [0001] The invention relates to a sandwich-type nucleic acid aptamer electrochemical sensor based on a copper-based-metal organic framework material label and a preparation method thereof, which is used for the detection of β-amyloid oligomers, and belongs to a new type of functional nanocomposite material And the field of biosensor detection technology. Background technique [0002] Metal-organic framework (MOF) materials are widely used in various fields, such as gas adsorption, separation, sensors, drug delivery, etc. and catalysis. In addition, the construction of electrochemical sensors based on MOFs has aroused people's strong interest (L.T.Liu, Y.L.Zhou, S. Liu, M.T.Xu, ChemElectroChem, Doi: 10.1002 / celc.201700931). Compared with other modifiers, the relatively large surface area of ​​MOFs can effectively improve the sensitivity of the sensor, and its unique structure and properties can also be used to immobilize other functional molecules. More imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/36
Inventor 周艳丽李聪明朱旭徐茂田
Owner SHANGQIU NORMAL UNIVERSITY
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