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Phosphorescence chemical sensor for qualitative homocysteine detection and use thereof

A photochemical sensor, homocysteine ​​technology, applied in the field of chemical sensors, can solve the problems of reporting iridium complexes

Inactive Publication Date: 2009-05-13
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, only a few iridium complexes have been used to detect oxygen concentration and calcium ions, and there are no reports on iridium complexes for the detection of amino acids

Method used

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  • Phosphorescence chemical sensor for qualitative homocysteine detection and use thereof
  • Phosphorescence chemical sensor for qualitative homocysteine detection and use thereof
  • Phosphorescence chemical sensor for qualitative homocysteine detection and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of iridium complex: weigh IrCl 3 ·3H 2 O (5.52mmol) and the corresponding cyclometalated C^N ligand 4-(2-pyridine)-benzaldehyde (11.04mmol) were added to the double-necked flask, vacuumed on the double-row tube-filled with nitrogen-vacuumized , cycle three times, and finally protect the reaction system with nitrogen. After injecting a mixture of 2-ethoxyethanol and water (3:1, v / v) with a syringe, the reaction mixture was heated to 110° C., stirred for about 24 hours, and a precipitate formed. After the reaction was stopped, the reaction mixture was cooled to room temperature, and a precipitate was obtained by filtration. The resulting precipitate was washed with water and ethanol respectively to obtain a red solid iridium dichloro bridge compound (pba) 2 Ir(μ-Cl) 2 Ir(pba) 2 . Weigh iridium dichloride bridge compound (0.16mmol) and acetylacetone ligand (0.32mmol) and join in the double-necked flask, vacuumize-fill nitrogen-vacuumize on the double-row ...

Embodiment 2

[0025] UV spectrum test of the iridium complex response to homocysteine: Dissolve the target iridium complex in a buffer solution of DMSO and HEPES, the concentration of HEPES is 50mmol / L, the volume ratio of the two is 9:1, and the pH of the mixed solution is The value is 7.2, the concentration of the iridium complex is 20 μmol / L, and then an equal volume of homocysteine ​​solution prepared by 0-200 eq HEPES buffer is added. After equilibration, measure the UV spectrum. See the experimental results figure 1 .

Embodiment 3

[0027]Phosphorescence spectrum test of the response of iridium complexes to homocysteine: Dissolve the target iridium complex in a buffer solution of DMSO and HEPES, the concentration of HEPES is 50mmol / L, the volume ratio of the two is 9:1, and the pH of the mixed solution is 7.2. The concentration of the iridium complex is 20 μmol / L, and then an equal volume of homocysteine ​​solution prepared in 0-200 eq HEPES buffer is added. After equilibration, the phosphorescence spectrum was measured. See the experimental results figure 2 .

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Abstract

The invention relates to the technical field of chemical sensor, in particular to a phosphorescent chemical sensor for qualitatively detecting homocysteine and an application thereof. Aldehyde group of iridium complex can take cyclization with amido and hydrosulphonyl in the homocysteine to be transformed into hexahydroxy heterocycle and transformed into another compound. The original iridium complex is conjugated with a benzene ring and the aldehyde group and represents as weak red fluorescence while the compound obtained after reaction is not conjugated with the aldehyde group and represents as strong green fluorescence. Ultraviolet and phosphorescent spectrum is used for detecting the result that the complex identifies the homocysteine, thereby showing that the complex has specific response towards the homocysteine, has high sensitivity and can be identified by naked eye, and furthermore, the complex can distinguish cysteine which has a structure very similar as the homocysteine.

Description

technical field [0001] The invention relates to the technical field of chemical sensors, in particular to a phosphorescent chemical sensor for qualitatively detecting homocysteine ​​and its application. Background technique [0002] In recent years, the clinical application of homocysteine ​​is mainly used as a risk indicator for cardiovascular diseases, especially coronary atherosclerosis and myocardial infarction, and its concentration is directly proportional to the risk of the disease. In addition, according to the metabolic characteristics of homocysteine, vitamin B12, folic acid deficiency, and hereditary N5 methyltetrahydrofolate methyltransferase and cystathionine-β-synthase gene defects are also related to homocysteine ​​concentration. There is a close relationship, but the relationship between these factors and cardiovascular disease needs further study. [0003] At present, the two main detection methods for homocysteine ​​are high performance liquid chromatograp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C07F15/00
Inventor 李富友陈绘丽刘倩黄春辉
Owner FUDAN UNIV
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