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Bacillus amyloliquefaciens and its bacterial depressant and use

A technology of amylolytic spores and antibacterial agents, applied in the field of microorganisms, can solve the problems of quality reduction, residue, and enrichment of aquatic products, and achieve the effects of controlling occurrence and spread, reducing occurrence and spread, and reducing mortality

Active Publication Date: 2017-01-11
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of antibiotics can effectively control the occurrence and spread of farmed animal diseases, there are blind use and drug abuse in actual production, and the abuse of antibiotics in aquaculture has many negative effects
First, the blind use of antibiotics seriously interferes with the normal growth and reproduction of beneficial microorganisms in the breeding environment and the intestinal tract of aquatic animals, destroying the microecological balance
Second, in aquaculture, the long-term large-scale and irregular use of antibiotics leads to the susceptibility of aquatic animals to pathogenic microorganisms, and more seriously, it leads to the enhancement of drug resistance of pathogenic bacteria, and the emergence of a large number of drug-resistant bacteria and even superbugs
Third, the use of antibiotics will also lead to their residues and accumulation in aquatic animals, which will eventually endanger human health
Although the residual amount of antibiotics in aquatic products is very low, the potential harm to human health is very serious and has far-reaching effects
Fourth, antibiotic drug residues also cause the quality of aquatic products to decrease, which has become a limiting factor in the sales and export volume of aquatic products

Method used

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  • Bacillus amyloliquefaciens and its bacterial depressant and use
  • Bacillus amyloliquefaciens and its bacterial depressant and use
  • Bacillus amyloliquefaciens and its bacterial depressant and use

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Effect test

Embodiment 1

[0028] The species identification of embodiment 1 bacterial strain

[0029] Biological characteristics: The colony morphology of the strain on 2216E medium is: white, translucent, smooth and moist colonies, slightly raised, round and neat colonies; Gram-stained bacteria are purple, Gram-positive bacteria, rod-shaped; Alkaline phosphatase test positive; naphthol-AS-BI phosphohydrolase test negative; α-glucosidase test positive; carbohydrate test: mannitol positive; L-arabinose positive; D-ribose positive; D-xylose positive; D-glucose positive; D-fructose positive; D-mannose positive; inositol positive; mannitol positive; sorbitol positive; methyl-αD-glucopyranoside positive; N-acetylglucosamine positive; bitter Amygdalin positive; ARBULIN positive; escin iron citrate positive; salicin positive; D-cellobiose positive; D-maltose positive; D-lactose positive; D-melbiose positive; D-sucrose positive; D - positive for trehalose; positive for D-raffinose; positive for starch; positi...

Embodiment 2

[0034] Embodiment 2 Bacillus amyloliquefaciens V4 bacterium supernatant is to the inhibition of growth of Vibrio vulnificus CZ-A2 (Vibriovulnificus)

[0035] 1. Preparation of Bacillus amyloliquefaciens V4 bacterial agent supernatant:

[0036] Inoculate the Bacillus amyloliquefaciens V4 strain into 100 mL of LB medium, culture at 30°C for 24 hours to obtain the bacterial agent, centrifuge the bacterial agent at 8000r / min for 10 minutes to obtain the supernatant of the bacterial agent, and use the supernatant with a disposable needle Type filter to obtain sterile fermentation broth.

[0037] LB medium components: 10 parts by mass of peptone, 5 parts by mass of yeast extract, 10 parts by mass of sodium chloride, 975 parts by mass of distilled water, pH 7.2.

[0038] 2. Inhibitory effect of bacterial agent supernatant on the growth of Vibrio vulnificus (Vibrio vulnificus) CZ-A2:

[0039] Prepare the bacterial suspension of Vibrio vulnificus (Vibrio vulnificus) CZ-A2, apply 100 ...

Embodiment 3

[0040] Embodiment 3, Bacillus amyloliquefaciens V4 bacterium supernatant is to the inhibition of the growth of sodium vibrio (Vibrionatriegens FS-1)

[0041] 1. Preparation of Bacillus amyloliquefaciens V4 bacterial agent supernatant:

[0042] Prepare the Bacillus amyloliquefaciens V4 bacterium supernatant according to step 1 in the embodiment 2.

[0043] 2. The inhibitory effect of bacterial agent supernatant on the growth of Natriegos vibrio (Vibrio natriegens FS-1):

[0044] Prepare a bacterial suspension from Vibrio natriegens, 100 μl of the bacterial suspension is coated on an LB solid plate, a sterile filter paper sheet with a diameter of 6 mm is placed on the surface of the plate, and 10 μl of sterile fermentation broth of Bacillus amyloliquefaciens V4 is added dropwise Place the plate on a piece of sterile filter paper and place it in a 30°C incubator for 24 hours. Observation shows the inhibition zone produced by Bacillus amylobacter V4 against Vibrio natrium, and t...

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Abstract

The invention relates to Bacillus amyloliquefaciens V4. The Bacillus amyloliquefaciens V4 is preserved in the China general microbiological culture collection center and has a preservation number of CGMCC NO. 10149. The Bacillus amyloliquefaciens V4 strain and / or microbial agent can produce bacteriocin for antagonizing vibrio pathogens and pathogenic aeromonas at seedling growing and culture temperatures of common culture water, inhibit vibrio vulnificus, vibrio natriegen, vibrio harveyi, black sea vibrio, aeromonas hydrophila and aeromonas salmonicida growth and breeding, effectively control aquatic animal infectious disease generation and propagating caused by vibrio pathogens and pathogenic aeromonas, reduce aquatic animal mortality and a forage coefficient and promote aquatic animal weight gain.

Description

technical field [0001] The invention belongs to the field of microorganisms, and more specifically relates to a strain of bacillus amyloliquefaciens, a bacteriostatic agent prepared therefrom and uses thereof. Background technique [0002] my country is a big aquaculture country in the world, with an annual aquaculture output of more than 50 million tons, accounting for 71% of the world's total output. However, the loss of output caused by aquatic diseases accounts for more than 15% every year, and the economic loss is as high as 150 billion yuan. There are more than 100 known aquaculture diseases and more than 300 pathogenic species, of which bacterial pathogenic species account for 54.9%. Pathogenic Vibrio and Aeromonas salmonicida are the most important pathogenic bacteria in infectious diseases of aquatic animals. The disease caused by Vibrio has a wide epidemic area and a high incidence rate, which has caused great harm to the aquaculture industry. Vibrio anguillarum...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00C02F3/34C12R1/07
Inventor 刘志培高喜燕刘缨苗莉莉
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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