Bacillus amyloliquefaciens and its bacterial depressant and use
A technology of amylolytic spores and antibacterial agents, applied in the field of microorganisms, can solve the problems of quality reduction, residue, and enrichment of aquatic products, and achieve the effects of controlling occurrence and spread, reducing occurrence and spread, and reducing mortality
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Embodiment 1
[0028] The species identification of embodiment 1 bacterial strain
[0029] Biological characteristics: The colony morphology of the strain on 2216E medium is: white, translucent, smooth and moist colonies, slightly raised, round and neat colonies; Gram-stained bacteria are purple, Gram-positive bacteria, rod-shaped; Alkaline phosphatase test positive; naphthol-AS-BI phosphohydrolase test negative; α-glucosidase test positive; carbohydrate test: mannitol positive; L-arabinose positive; D-ribose positive; D-xylose positive; D-glucose positive; D-fructose positive; D-mannose positive; inositol positive; mannitol positive; sorbitol positive; methyl-αD-glucopyranoside positive; N-acetylglucosamine positive; bitter Amygdalin positive; ARBULIN positive; escin iron citrate positive; salicin positive; D-cellobiose positive; D-maltose positive; D-lactose positive; D-melbiose positive; D-sucrose positive; D - positive for trehalose; positive for D-raffinose; positive for starch; positi...
Embodiment 2
[0034] Embodiment 2 Bacillus amyloliquefaciens V4 bacterium supernatant is to the inhibition of growth of Vibrio vulnificus CZ-A2 (Vibriovulnificus)
[0035] 1. Preparation of Bacillus amyloliquefaciens V4 bacterial agent supernatant:
[0036] Inoculate the Bacillus amyloliquefaciens V4 strain into 100 mL of LB medium, culture at 30°C for 24 hours to obtain the bacterial agent, centrifuge the bacterial agent at 8000r / min for 10 minutes to obtain the supernatant of the bacterial agent, and use the supernatant with a disposable needle Type filter to obtain sterile fermentation broth.
[0037] LB medium components: 10 parts by mass of peptone, 5 parts by mass of yeast extract, 10 parts by mass of sodium chloride, 975 parts by mass of distilled water, pH 7.2.
[0038] 2. Inhibitory effect of bacterial agent supernatant on the growth of Vibrio vulnificus (Vibrio vulnificus) CZ-A2:
[0039] Prepare the bacterial suspension of Vibrio vulnificus (Vibrio vulnificus) CZ-A2, apply 100 ...
Embodiment 3
[0040] Embodiment 3, Bacillus amyloliquefaciens V4 bacterium supernatant is to the inhibition of the growth of sodium vibrio (Vibrionatriegens FS-1)
[0041] 1. Preparation of Bacillus amyloliquefaciens V4 bacterial agent supernatant:
[0042] Prepare the Bacillus amyloliquefaciens V4 bacterium supernatant according to step 1 in the embodiment 2.
[0043] 2. The inhibitory effect of bacterial agent supernatant on the growth of Natriegos vibrio (Vibrio natriegens FS-1):
[0044] Prepare a bacterial suspension from Vibrio natriegens, 100 μl of the bacterial suspension is coated on an LB solid plate, a sterile filter paper sheet with a diameter of 6 mm is placed on the surface of the plate, and 10 μl of sterile fermentation broth of Bacillus amyloliquefaciens V4 is added dropwise Place the plate on a piece of sterile filter paper and place it in a 30°C incubator for 24 hours. Observation shows the inhibition zone produced by Bacillus amylobacter V4 against Vibrio natrium, and t...
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