Biosensing detection method used for identifying salmo salar and rainbow trout

Abstract

The invention discloses a biosensing detection method used for identifying a salmo salar and a rainbow trout. The biosensing detection method comprises the following steps: the step 1 of extracting genome DNA of a sample to be detected, using a specific primer pair of the salmo salar to carry out PCR reaction with the genome DNA of the sample to be detected as a template and obtaining a PCR amplification product I of the sample to be detected; the step 2 of adding the PCR amplification product I obtained in the step 1 to the surface of an electrode decorated by an anchoring acceptor for reaction; the step 3 of detecting an electrochemistry signal of an electrochemistry activity labeled molecule and deciding that the sample to be detected comes from the salmo salar if the electrochemistry signal of the electrochemistry labeled molecule exists. The specific primer pair of the salmo salar comprises the electrochemistry activity labeled molecule and an anchoring labeled molecule on the upstream of a base sequence respectively, and the anchoring labeled molecule can be captured by the anchoring acceptor to enable the PCR amplification product with the anchoring labeled molecule to be captured by the surface of the electrode. The method is suitable for fish samples of any growth stage and in any shape.

Description

technical field [0001] The invention belongs to the technical field of biosensing, and relates to a detection technology for identifying the species of fish roe, fry, adult fish, fish meat and products thereof, in particular to a biosensing detection technology for identifying Atlantic salmon and rainbow trout method. Background technique [0002] Atlantic salmon (Salmo salar), belonging to the order Salmoniformes, the family Salmonidae, and the genus Salmo, originally lived in the temperate and subarctic regions of the northern Atlantic Ocean. It was successfully cultured in Norway in the 1960s. At present, it has become a worldwide farmed fish. It mainly breeds and produces commercial fish in the form of seawater cages. It is called "Norwegian salmon" in my country's aquatic product market, and its sales price is relatively high. [0003] Rainbow trout (Oncorhynchus mykiss), belonging to Salmoniformes (Salmoniformes), Salmonidae (Salmonidae), salmon genus (Oncorhynchus), t...

AI Technical Summary

Problems solved by technology

[0004] The production of Atlantic salmon in my country is relatively low, but salmon sashimi, smoked salmon and other products made from it are very popular with consumers, so some unscrupulous farmers and operators use rainbow trout to pretend to be Atlantic salmon: some use rainbow trout and The close relationship in Atlantic salmon taxonomy, the title of rainbow trout as "freshwater salmon" is confusing and sold at a high price; some people add pigments to rainbow trout feed, so that the originally white and blue rainbow trout meat presents a color similar to that of Atlantic salmon It is orange-red in color and sold as imported "Norwegian salmon", which seriously infringes consumers' right to know, and even has health risks such as illegal coloring.

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