37 results about "Genus Oncorhynchus" patented technology

The invention discloses a biosensing detection method used for identifying a salmo salar and a rainbow trout. The biosensing detection method comprises the following steps: the step 1 of extracting genome DNA of a sample to be detected, using a specific primer pair of the salmo salar to carry out PCR reaction with the genome DNA of the sample to be detected as a template and obtaining a PCR amplification product I of the sample to be detected; the step 2 of adding the PCR amplification product I obtained in the step 1 to the surface of an electrode decorated by an anchoring acceptor for reaction; the step 3 of detecting an electrochemistry signal of an electrochemistry activity labeled molecule and deciding that the sample to be detected comes from the salmo salar if the electrochemistry signal of the electrochemistry labeled molecule exists. The specific primer pair of the salmo salar comprises the electrochemistry activity labeled molecule and an anchoring labeled molecule on the upstream of a base sequence respectively, and the anchoring labeled molecule can be captured by the anchoring acceptor to enable the PCR amplification product with the anchoring labeled molecule to be captured by the surface of the electrode. The method is suitable for fish samples of any growth stage and in any shape.

The invention discloses an industrialized fry breeding method for marine oncorhynchus mykiss. The method comprises steps as follows: (1), hatching: eyed eggs are hatched in an indoor fresh water flowing manner, and a hatching room keeps dark; (2), breeding of fish larvae and juveniles: the fish larvae and juveniles are bred in the indoor fresh water flowing manner, the water temperature is kept in a range from 10 DEG C to 14 DEG C, the new water addition amount of a single pool is 10-15 L / min / 10,000 fish larvae and juveniles, and the breeding density in the water flowing mode does not exceed 15 kg / m<3>; (3), fry breeding: the marine oncorhynchus mykiss fries are bred by adopting indoor closed circulating fresh water, the water temperature is kept in a range from 14 DEG C to 18 DEG C, the water circulating amount of a circulating water breeding pool is 15-25 times / day / pool, the illumination intensity is kept in 800-1,000 lux, and the 24 hours of illumination intensity is kept. The fry breeding process of the marine oncorhynchus mykiss is performed indoors and is not influenced by weather, year-round production can be realized, the water quality condition is stable and controllable, the survival rate of the bred fries is high, and high-density large-scale breeding can be realized.

An anti-hypertensive fish protein hydrolysate is provided, wherein the fish is of the genus Salmo or Oncorhynchus, and wherein the fish protein hydrolysate comprises at least 1 peptide selected from the group consisting of: Leu-Ala-Phe, Leu-Thr-Phe, Ile-Ile-Phe, Leu-Ala-Tyr, Ile-Ala-Tyr, Val-Phe-Tyr, Tyr-Ala-Tyr, Val-Leu-Trp, Ile-Ala-Trp, Tyr-Ala-Leu and Tyr-Asn-Arg. Methods of making and methods for using such fish protein hydrolysates are also provided.

The invention belongs to the technical field of cold water fish farming equipment manufacturing, relates to a fish-farming hold arranged on a deck, in particular to an on-board fish-farming hold for breeding oncorhynchus. The main structure of the on-board fish-farming hold for breeding the oncorhynchus comprises a hold cover preventing other water sources from entering the fish hold and a hold body providing a suitable living environment for the oncorhynchus, wherein the hold cover comprises an edge fish hold upper cover, a middle fish hold upper cover, drain holes, a hatch tank, an upper cover lifting hole, a fixing groove, a diversion notch and a waterproof connecting groove; the hold body comprises a pipe hole, a water inlet pipe, a water chamber, a water inlet pipe diversion port, a vertical insertion pipe, a drain pipe, an overflow pipe, a drain pipe valve, an overflow pipe valve, a drain pipe port, a sewage collection chamber and a blind plate flange; the hatch tank is located on the upper edge of the fish hold body, so that the edge fish hold upper cover and the middle fish hold upper cover are convenient to take and place, and the bottom of the water chamber is an invertedcone bottom. The on-board fish-farming hold for breeding the oncorhynchus has the advantages that the main structure is simple, the design concept is ingenious, the production and use are safe and convenient, the preparation cost is low, the application environment is good, and the market prospect is wide.

The invention belongs to the field of aquaculture, and discloses a breeding method capable of improving disease resistance of oncorhynchus mykiss. The method comprises the following steps: washing a pond pasted with cement, and disinfecting the pond by using lime water; sprinkling a fertilizer on the water surface of the pond; selecting fry with equal sizes and a body length of 3-5 cm, and throwing the fry according to a fry throwing amount of 5000-10000 / mu; after the fry are thrown, performing feeding by using fry bait, wherein feeding is performed once separately in the morning and evening,and feeding is performed according to a feed amount of 5-10% of a body weight of the oncorhynchus mykiss every time; after the fry are mature, performing feeding by using mixed feed, wherein feeding is performed according to a feed amount of 10-15% of a body weight of the oncorhynchus mykiss every time; and performing comprehensive sterilization on the fish pond for one time every month, and regularly removing sediments at the bottom of the pond every month. The method provided by the invention has simple operation, and the oncorhynchus mykiss has high autoimmune function and disease resistance, a high survival rate, a high growth speed and high economic benefits.

The invention discloses a method for controlling the synchronous maturity of brook trout and rainbow trout. The method comprises the following steps that female rainbow trout and male brook trout are cultured in a separated mode; when the ovaries of the female rainbow trout are developed to the end of the III period and the beginning of the IV period, stimulation by running water is conducted for 2 hours one time every 3 days, and the water temperature in a culture pond is decreased to 6 DEG C; meanwhile, the average sunlight irradiation time of the male brook trout is adjusted to 5.8 hours, the male brook trout is subjected to sunlight irradiation one time every day, and stimulation by the running water is conducted for 6 hours one time every day; the female rainbow trout is fed with fresh fish one time every week; the male brook trout is fed with the fresh fish one time every day, the female rainbow trout is fed with commercial feed two times every day, and the male brook trout is fed with granulated feed two times every day. By means of the method, the synchronous maturity rate of the brook trout and the rainbow trout can be increased to 85.7%, it is ensured that the amount of the rainbow trout is enough for hybridization, and the method is beneficial to popularizing the hybridization breeding work of the brook trout and the rainbow trout.

The invention discloses a biosensing detection method used for identifying a salmo salar and a rainbow trout. The biosensing detection method comprises the following steps: the step 1 of extracting genome DNA of a sample to be detected, using a specific primer pair of the salmo salar to carry out PCR reaction with the genome DNA of the sample to be detected as a template and obtaining a PCR amplification product I of the sample to be detected; the step 2 of adding the PCR amplification product I obtained in the step 1 to the surface of an electrode decorated by an anchoring acceptor for reaction; the step 3 of detecting an electrochemistry signal of an electrochemistry activity labeled molecule and deciding that the sample to be detected comes from the salmo salar if the electrochemistry signal of the electrochemistry labeled molecule exists. The specific primer pair of the salmo salar comprises the electrochemistry activity labeled molecule and an anchoring labeled molecule on the upstream of a base sequence respectively, and the anchoring labeled molecule can be captured by the anchoring acceptor to enable the PCR amplification product with the anchoring labeled molecule to be captured by the surface of the electrode. The method is suitable for fish samples of any growth stage and in any shape.

The invention provides a hypothermic anesthesia transportation method for live Oncorhynchus mykiss, the method comprising following steps: 1) feed deprivation: food provisioning for Oncorhynchus mykiss is stopped for 2-3 days before transportation; 2) cooling and anesthesia treatment: water tanks are filled with cooled aquaculture water of 1-2 DEG C; the water temperature is maintained; anesthetic is added to the water and mixed well. The Oncorhynchus mykiss deprived of food is weighed and then put in the aquaculture water and quick cooling and anesthesia are performed. The Oncorhynchus mykiss is taken out of water surface when the activity ability of the fish is nearly exhausted. By this time, the fish bodies are in the state of hypothermic anesthesia and then they are to be encased. 3) the Oncorhynchus mykiss are transported by packing transportation or water track transportation. After arriving at a destination, The Oncorhynchus mykiss are brought back to life and the temperature of aquaculture water is gradually increased to normal level. The hypothermic anesthesia transportation method has following beneficial effects: no matter how much the transportation volume of live fish is, the transportation method can be performed with low cost, simple, flexible and easy operation; and the economic benefit can be guaranteed by high rate of survival of fish after transportation.

The invention discloses an industrialized fry breeding method for marine oncorhynchus mykiss. The method comprises steps as follows: (1), hatching: eyed eggs are hatched in an indoor fresh water flowing manner, and a hatching room keeps dark; (2), breeding of fish larvae and juveniles: the fish larvae and juveniles are bred in the indoor fresh water flowing manner, the water temperature is kept in a range from 10 DEG C to 14 DEG C, the new water addition amount of a single pool is 10-15 L / min / 10,000 fish larvae and juveniles, and the breeding density in the water flowing mode does not exceed 15 kg / m<3>; (3), fry breeding: the marine oncorhynchus mykiss fries are bred by adopting indoor closed circulating fresh water, the water temperature is kept in a range from 14 DEG C to 18 DEG C, the water circulating amount of a circulating water breeding pool is 15-25 times / day / pool, the illumination intensity is kept in 800-1,000 lux, and the 24 hours of illumination intensity is kept. The fry breeding process of the marine oncorhynchus mykiss is performed indoors and is not influenced by weather, year-round production can be realized, the water quality condition is stable and controllable, the survival rate of the bred fries is high, and high-density large-scale breeding can be realized.

The invention discloses a Chinese herbal medicine feed additive for preventing oncorhynchus mykiss from suffering from a fin-rot disease and use of the Chinese herbal medicine feed additive. The additive comprises the components in parts by weight: 18-22 parts of lemna minor, 20-25 parts of heartleaf houttuynia herbs, 10-14 parts of chinaberry bark, 13-17 parts of common cnidium fruits, 8-12 parts of radix scutellariae, 11-14 parts of Chinese magnoliavine fruits, 6-8 parts of hedyotis diffusa, 7-9 parts of fructus forsythiae, 5-7 parts of wild chrysanthemum flowers, 3-5 parts of radix bupleuri, and 1-3 parts of radix angelicae sinensis. The Chinese herbal medicine feed additive has the beneficial effects that the components are reasonable in compatibility, and the additive has remarkable synergy effects, has effects of resisting bacteria, removing toxicity and disinfecting insects, can effectively prevent the fin-rot disease and can improve the immunity of the organisms of the oncorhynchus mykiss. The additive belongs to a purely natural substance, so that after the additive is used for a long term, the problems of drug resistance, drug residues and the like are not liable to cause. The additive is convenient to use, the oncorhynchus mykiss does not need to be fished out to be applied with drugs, and the damage to the oncorhynchus mykiss in the operation process is reduced.

The invention provides a production method of Oncorhynchus Mykiss triploid fry. The production method including fertilizing fertilized eggs of Oncorhynchus Mykiss for 12-14 min at the temperature of 7 DEG C to transfer to 240mg / L 6-DMAP (6-Dimethylaminopurine) solution at temperature at 12-14 DEG C for soaking for 14-16 min, and then transferring the fertilized eggs to hatching water to hatch normally. After around 50 days, the Oncorhynchus Mykiss triploid fry are hatched from the fertilized eggs. Eyeing rate of the fertilized eggs during hatching is more than 68%, hatching rate of the fry reaches 90%, the fry are detected by a flow cytometry, rate of triploid reaches 81%. By the aid of the production method of the Oncorhynchus Mykiss triploid fry, the problems of precocious puberty, individual miniaturization, disease breeding and the like on cultivation individuals due to long-term inbreeding and lack of systematic select breeding during cultivation of the Oncorhynchus Mykiss, and the production method has good market value.