Oncorhynchus keta walbaum narcosis method
A technology of autumn salmon and anesthetic, which is applied in the field of anesthesia for autumn salmon, achieves the effects of high safety factor, protection of fish welfare, and reduction of injuries
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Embodiment 1
[0018] The anesthesia method of mid-autumn salmon in the present embodiment mainly comprises the following steps:
[0019] 1) Preparation: add the breeding water in the breeding system to the anesthesia box 1 and the recovery box, open the oxygenation equipment in the box, and ensure that the dissolved oxygen content in the water is above 7mg / L; add ice packs to the anesthesia box 1 and the recovery box to ensure The anesthesia water temperature and recovery water temperature are the same as or slightly lower than the culture water temperature. Add the anesthetic MS-222 to the culture water in the anesthesia box 1 and dissolve it completely until the concentration of the anesthetic reaches 0.1 g / L.
[0020] 2), anesthesia: fish 20 autumn salmon seedlings with a body weight range of 40-80g into the anesthesia box 1 in turn and begin to record the anesthesia time. The anesthesia box 1 is covered with a nylon net to prevent the fish from jumping out of the water during the anesth...
Embodiment 2
[0025] Change the concentration of anesthetic MS-222 into 0.2g / L in step 1, implement embodiment 2 in the same manner as embodiment 1.
Embodiment 3
[0027] Change the concentration of anesthetic MS-222 into 0.3g / L in step 1, implement embodiment 3 in the same manner as embodiment 1.
[0028] The results are shown in Table 1.
[0029] Table 1
[0030]
[0031] It can be seen from Table 1 that when the concentration of the anesthetic agent is in the range of 0.1-0.2g / L, with the increase of the concentration of the anesthetic agent, the anesthesia time of autumn salmon is shortened, the recovery time is increased, and there are occasional cases of early awakening during the out-of-water operation. Survival rate is all 100% (embodiment 1, embodiment 2) after resuscitating 24h, and analysis is because anesthesia is not thorough and causes; During the out-of-water operation, no early awakening was found, but the resuscitated autumn salmon died within 24 hours (Example 3), which was analyzed due to excessive anesthesia.
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