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Molecular marker primer and method for identifying North Pacific salmon and rainbow trout

A technology of salmon and molecular markers, applied in the field of molecular biology, can solve the problem of mixed germplasm of salmon genus and achieve stable results, high accuracy, and easy operation

Inactive Publication Date: 2018-02-16
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION +1
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a molecular marker primer and method for distinguishing salmon and rainbow trout, which helps to solve the problem of mixed germplasm of salmon genus fish. The method of the present invention is simple to operate and easy to master. high accuracy

Method used

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Embodiment Construction

[0017] The technical solution of the present invention will be further explained by examples below, but the protection scope of the present invention is not limited by any form of examples.

[0018] A molecular marker method for distinguishing North Pacific salmon and rainbow trout, comprising the following steps:

[0019] (1) Select salmon and rainbow trout collected from the survey of swimming animals in the North Pacific to form a salmon sample group and a rainbow trout sample group, and extract the total genomic DNA of the two sample groups;

[0020] (2) Carrying out PCR amplification reaction with the extracted total genomic DNA as template;

[0021] (3) Purify and sequence the successfully amplified PCR products, and compare the sequencing results.

[0022] The obtained molecular marker primer sequences were MiFish-U-F: 5'-GTCGGTAAAACTCGTGCCAGC-3'; MiFish-U-R: 5'-CATAGTGGGGTATCTAATCCCAGTTTG-3'.

[0023] Take 1ul of the total genomic DNA of the two sample populations, a...

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Abstract

The invention relates to a molecular marker primer and a method for identifying North Pacific salmon and rainbow trout. Sequences of the marker primer are: MiFish-U-F:5'-GTCGGTAAAACTCGTGCCAGC-3'; andMiFish-U-R:5'-CATAGTGGGGTATCTAATCCCAGTTTG-3'. The molecular marker primer provided by the invention helps to solve the problem that germplasms of oncorhynchus suckley fishes are mixed, and the methodprovided by the invention is simple to operate, easy to master and high in accuracy.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a molecular marker primer and a method for identifying North Pacific salmon and rainbow trout. Background technique [0002] Rainbow trout belongs to the ray-finned fish class, Salmoniformes, Salmonidae, and salmon genus. Its external shape is similar to that of salmon: long body, slightly flattened on the side. The kiss is blunt. Mouth large, slightly oblique upward. Jaws and teeth well developed. eyes big. Body covered with round scales. Dorsal fin starting point anterior to pelvic fin, adipose fin behind it, dorsal fin rays IV—9-12. Pectoral fins small, not reaching pelvic fins, pectoral fin rays I, 11-13. Pelvic fin grayish white, sides silvery white. Anal fin rays II—8-12. Caudal fin forked. The back of the body is pale green or yellowish green, with varying numbers of small black spots scattered on the head, back, sides of the body and each fin. Gill rakers 20; la...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 李渊张静张然林龙山
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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