Oncorhynchus ganoderma lucidum and its cultivation method
A cultivation method and a hook kiss technology are applied in the field of ganoderma lucidum and its cultivation, which can solve the problems of restricting the application of hook kiss, low safety index and the like, and achieve the effects of improving the safety index, reducing the toxicity and having a good application prospect.
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Embodiment 1
[0039] The cultivation method of ganoderma ganoderma specifically comprises the following steps:
[0040] (1) Inoculate the strains of Chizhi on the slant medium made from the seed medium, and after the mycelium grows on the slant, obtain the mother fungus; transfer the mother fungus slant to a new seed medium, at 28 ° C Under constant temperature cultivation 3d, obtain the bacterial classification of expanded culture; Wherein, described seed culture medium is PDA medium, is made by potato 200g, glucose 20g, agar 15g and 1000ml distilled water;
[0041] (2) Wash the expanded cultured strains with 3-5mL 0.9% sterile NaCl solution to make a bacterial suspension;
[0042] (3) Grind the dried Glycyrrhizae decoction pieces through a 10-mesh sieve, add water until the water content of the dried Glycorrhizae decoction pieces after crushing and sieving is 60%, mix well and fully moisten, and obtain Glycorrhizae decoction pieces granules ;
[0043] (4) Put 400g of the stalk decoction...
Embodiment 2
[0046] The cultivation method of ganoderma ganoderma specifically comprises the following steps:
[0047] (1) Inoculate the strains of Chizhi on the slant medium made from the seed medium, and after the mycelium grows on the slant, obtain the mother fungus; transfer the mother fungus slant to a new seed medium, at 28 ° C Under constant temperature cultivation 3d, obtain the bacterial classification of expanded culture; Wherein, described seed culture medium is PDA medium, is made by potato 200g, glucose 20g, agar 15g and 1000ml distilled water;
[0048] (2) Wash the expanded cultured strains with 3-5mL 0.9% sterile NaCl solution to make a bacterial suspension;
[0049] (3) Grinding the dried Glycyrrhizae decoction pieces through a 10-mesh sieve, adding bran, light calcium carbonate and brown sugar to the dried Glycyrrhizae decoction pieces after crushing and sieving to obtain a mixture, adding water to the water content of the mixture 60%, mix well and fully moisten, and get ...
Embodiment 3
[0053] The cultivation method of ganoderma ganoderma specifically comprises the following steps:
[0054] (1) Inoculate the strains of Versicolor versicolor on the slant medium made by the seed medium, and after the mycelium is covered with the slant, obtain the mother fungus; transfer the mother fungus slant to the new seed medium, and in 28 Cultivate at a constant temperature for 3 d under ℃ to obtain the strains of expanded culture; wherein, the seed culture medium is a PDA medium made of 200 g of potatoes, 20 g of glucose, 15 g of agar and 1000 ml of distilled water;
[0055] (2) Wash the expanded cultured strains with 3-5mL 0.9% sterile NaCl solution to make a bacterial suspension;
[0056] (3) Grinding the dried Glycyrrhizae decoction pieces through a 10-mesh sieve, adding bran, light calcium carbonate and brown sugar to the dried Glycyrrhizae decoction pieces after crushing and sieving to obtain a mixture, adding water to the water content of the mixture 60%, mix well ...
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