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Production method of Oncorhynchus Mykiss triploid fry

A technique for steelhead trout and triploids, which is applied in the field of preparation of steelhead trout triploid seedlings, can solve the problems of sensitivity to chemical substances, lack of 6-dimethylaminopurine, and high water quality requirements for growth environments

Inactive Publication Date: 2015-01-07
BEIJING FISHERIES RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 6-Dimethylaminopurine (N6-Dimethyladenine, 6-DMAP) has achieved good results in the induction of polyploidy in various shellfish, but because salmon trout have high requirements on the water quality of the growth environment and are particularly sensitive to chemical substances, So far, there is no successful report on the application of 6-dimethylaminopurine in triploid induction of salmon and trout

Method used

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  • Production method of Oncorhynchus Mykiss triploid fry
  • Production method of Oncorhynchus Mykiss triploid fry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Method for preparing steelhead trout triploid seed (1)

[0026] Stimulation by water flow, control of light, and enhanced compound feed for broodstock were used to mature the broodstock of steelhead trout. The water temperature for broodstock breeding of steelhead trout is 10°C, and the water exchange volume of the running pool is 2h / time.

[0027] The eggs are fertilized by dry method. Squeeze the eggs of mature female steelhead trout into a clean porcelain basin first, and then squeeze the semen of 2 male steelhead trout into the water basin. 10°C, stir immediately, add hatching water with a volume ratio of semen to hatching water of 1:2500, stir gently, pour out the water after 1 min, repeat 3 times, clean the semen, and place it for treatment.

[0028] After steelhead trout eggs were fertilized for 12 minutes, the fertilized eggs were quickly transferred to the 6-DMAP solution with a concentration of 240mg / L and a temperature of 12°C for soaking treatmen...

Embodiment 2

[0030] Example 2 Method for preparing triploid seedlings of steelhead trout (2)

[0031] Stimulation by water flow, control of light, and enhanced compound feed for broodstock were used to mature the broodstock of steelhead trout. The water temperature for broodstock breeding of steelhead trout is 13°C, and the water exchange volume in the flowing pool is 2h / time.

[0032] The eggs are fertilized by dry method. Squeeze the eggs of mature female steelhead trout into a clean porcelain basin first, and then squeeze the semen of 2 male steelhead trout into the water basin. 9°C, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1:3000, stir gently, pour out the water after 1.5 minutes, repeat 3 times, clean the semen, and place it for processing.

[0033] After steelhead trout eggs were fertilized for 13 minutes, the fertilized eggs were quickly transferred to 6-DMAP solution with a concentration of 220mg / L and a temperature of 12°C for...

Embodiment 3

[0035] Example 3 Method for preparing triploid seedlings of steelhead trout (3)

[0036] Stimulation by water flow, control of light, and enhanced compound feed for broodstock were used to mature the broodstock of steelhead trout. The water temperature for broodstock breeding of steelhead trout is 12°C, and the water exchange volume of the running pool is 2h / time.

[0037] The eggs are fertilized by dry method. Squeeze the eggs of mature female steelhead trout into a clean porcelain basin first, and then squeeze the semen of 2 male steelhead trout into the water basin. 10°C, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1.5:3000, stir gently, pour out the water after 1 min, repeat 3 times, clean the semen, and place it for processing.

[0038] After steelhead trout eggs were fertilized for 14 minutes, the fertilized eggs were quickly transferred to 6-DMAP solution with a concentration of 240mg / L and a temperature of 12°C for so...

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Abstract

The invention provides a production method of Oncorhynchus Mykiss triploid fry. The production method including fertilizing fertilized eggs of Oncorhynchus Mykiss for 12-14 min at the temperature of 7 DEG C to transfer to 240mg / L 6-DMAP (6-Dimethylaminopurine) solution at temperature at 12-14 DEG C for soaking for 14-16 min, and then transferring the fertilized eggs to hatching water to hatch normally. After around 50 days, the Oncorhynchus Mykiss triploid fry are hatched from the fertilized eggs. Eyeing rate of the fertilized eggs during hatching is more than 68%, hatching rate of the fry reaches 90%, the fry are detected by a flow cytometry, rate of triploid reaches 81%. By the aid of the production method of the Oncorhynchus Mykiss triploid fry, the problems of precocious puberty, individual miniaturization, disease breeding and the like on cultivation individuals due to long-term inbreeding and lack of systematic select breeding during cultivation of the Oncorhynchus Mykiss, and the production method has good market value.

Description

technical field [0001] The invention relates to the technical field of aquatic organism breeding, in particular to a method for preparing triploid seedlings of steelhead trout. Background technique [0002] Salmon trout is one of the main fish cultured in the world at present. Because of its own quality and special requirements for the living environment, it is a green, environmentally friendly and nutritious food, so it is very popular among consumers. Salmon trout is currently the main cold-water cultured fish in my country. With the rapid development of salmon trout farming in China, basic theoretical research is relatively lagging behind. Problems such as the degradation of broodstock germplasm of salmon trout lead to the degradation of seed quality and the decline in production efficiency have gradually become prominent. , thus restricting the sustainable development of salmon and trout farming. Using modern biotechnology to cultivate seedlings with fast growth, large i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 徐绍刚李文通田照辉杨晓飞杨贵强
Owner BEIJING FISHERIES RES INST
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