Preparation method of game fish triploid fries

Abstract

The invention provides a preparation method of game fish triploid fries. The preparation method comprises the following steps of: hybridizing a rainbow trout female parent fish and an arctic char male parent fish; transferring fertilized ova which have been fertilized for 20 minutes under the condition of 7 DEG C into a water body of 26 DEG C and performing heat shock for 20 minutes; then transferring into a water body of 7 DEG C to hatch for about 60 days; and hatching fish fries from the fertilized ova, wherein the eyed rate of the fertilized ova in the hatching period is over 75 percent, the hatching rate of the fries reaches over 90 percent, and the triploid rate of the obtained fries is 90-95 percent through detection of a flow cytometer. According to the preparation method of game fish triploid fries provided by the invention, the problems of sexual precosity, size miniaturization, disease breeding and the like of bred individuals due to long-term close breeding and shortage of systematic breeding in the rainbow trout culturing process are relieved, meanwhile, the hybrid triploid has the two advantages of the hybridization and the triploid, the growth speed and the disease resistance are obviously superior to those of the conventional diploid rainbow trout, and the preparation method has higher economic benefit and market value.

Description

technical field [0001] The invention relates to the technical field of aquatic organism breeding, in particular to a method for preparing a hybrid triploid seed of salmon and trout. Background technique [0002] Salmon and trout are currently one of the main farmed fish in the world. Under natural conditions, they mostly grow in low hot spring water or clear streams where the water temperature does not exceed 20°C all year round, the water quality is oligotrophic, and there is no chemical or organic pollution. , so it is also called cold water fish. Because salmonids have very high requirements on the quality of living water, they cannot survive with slight pollution. Therefore, salmon and trout are clean and free of any pollution. They are veritable pollution-free green food, and they are safe to eat. In addition, salmon trout is a high-protein, high-fat, low-cholesterol (very little cholesterol) fish. Compared with other fish, salmon and trout contain glycine, which is l...

AI Technical Summary

Problems solved by technology

The existing heat shock method for preparing purebred triploid rainbow trout is that under the condition of 26.5°C, the rate of eye formation of fertilized eggs is 67.80%-70.28%, and the triploid rate of fry can reach 50%-65%; At 28°C, the rate of fertilized eggs is 29.78%-32.75%, and the triploid rate of fry can reach 80.95%-83.18%. At 30°C, a very high triploid rate can be obtained, reaching 90%. above, but its hair eye rate is usually no more than 10%, and the seed deformity rate is high, and it cannot form large-scale production all the time. The ploidy rate or the survival rate of seedlings that can be used for production cannot be reached