Application of neutral sterilization electrochemical water in fish egg hatching
A neutral sterilization, electro-chemical water technology, applied in the application, climate change adaptation, fish farming and other directions, can solve the problems of environmental pollution in aquaculture waters, drug resistance of fish fry, rupture of egg membranes, etc., to improve the survival rate and hatching. rate, the effect of a wide range of application prospects
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Embodiment 1
[0025] The application and method of the present invention are suitable for hatching eggs of various fish, especially loach, sturgeon, rainbow trout, and koi.
[0026] (1) Oxytocin and other exogenous hormones such as pituitary gonadotropin and oxytocin are injected into the body cavity of the parent fish with a sterilized syringe and needle (No. The effect time of oxytocin, predicting its spawning time;
[0027] (2) 1-2 hours in advance, use the complete set of equipment to treat NaCl concentration ≥ 2‰ to prepare sterilized electrochemical water, in which the concentration of available chlorine is 50-150mg / L, the content of ozone is 2-5mg / L, hydrogen peroxide 10~35mg / L, ph value between 6.5-7.5, redox potential 800-1300Mv. The water quality of aquaculture water varies from place to place. In some areas, the hardness of the water body is relatively high. In some areas, ammonia nitrogen and nitrite exceed the standard. In some areas, the water body is eutrophic and the algae ...
Embodiment 2
[0035] In loach egg hatching, available chlorine mass concentration is 150mg / L, ozone content 2mg / L, hydrogen peroxide 35mg / L, pH value is 7.5, and redox potential is 1300mV, and other steps are as embodiment 1. Set up the pre-experiment and repeat the verification based on it as follows:
[0036] Four groups of electro-chemical water with different concentrations were designed and hatched in static water at different breeding periods. The control group (group D) chose the original pond water for incubation, and the electro-chemical water group used the treatment of the original pond water. Concentration groups have no parallel groups (see Table 1). The weight of hatching water and the number of loach eggs in each concentration group were consistent.
[0037] Table 1
[0038]
[0039] Pre-test results
[0040] Table 2
[0041]
Embodiment 3
[0043] The verification test of Example 2 was repeated.
[0044] On the basis of the pre-test, different concentrations of electrolytic water were used, and four times of repeated verification hatching experiments were carried out at different breeding periods. Each concentration group had three replicates, and 1000 loach eggs were placed in each replicate. In the third test, two groups of 15% concentration were set, one group was water production 12 hours in advance (before 15%), and the other group was water production 30 minutes before the test (after 15%), and then set water concentration on the basis of the 15% concentration group. Set 26% concentration group.
[0045] Experimental Design of Hatching of Loach Eggs Treated with Different Concentrations of Neutral Electrochemical Water
[0046] table 3
[0047]
[0048] Table 4
[0049]
[0050] Repeat verification test results
[0051] Table 4
[0052]
[0053]The results show that neutral electrolytic wate...
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