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Rainbow trout tetraploid breeding method

A technology of tetraploid and rainbow trout, which is applied in fish farming, application, climate change adaptation, etc., to achieve the effect of good adaptability and simple operation method

Inactive Publication Date: 2011-01-12
北京卧佛山庄养殖有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, also do not utilize biological method to obtain the report of rainbow trout tetraploid fish at present, need to utilize biological method to obtain the cultivation method of rainbow trout tetraploid fish on the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] This embodiment is a method for obtaining fertile tetraploid rainbow trout by intergenus hybridization with rainbow trout and char.

[0028] The method sequentially includes the following steps:

[0029] A. Take the ovum of rainbow trout and the sperm of char, and carry out fertilization treatment to obtain fertilized eggs;

[0030] Described rainbow trout (Oncorhynchus mykiss) and arctic salmon (Salvelinus fontinalis), these two salmonid fishes, they belong to salmon genus (Oncorhynchus) and salmon genus (Salvelinus) respectively; Described rainbow trout is sexually mature female, said char is a sexually mature male;

[0031] The rainbow trout and char are all obtained from the breeding base of Beijing Wofoshanzhuang Breeding Co., Ltd., or from other farms on the market;

[0032] The fertilization treatment is artificial dry fertilization, and the specific operation is: squeeze the eggs of the rainbow trout from the fis...

Embodiment 2

[0071] This embodiment is a preferred solution based on Embodiment 1. In this example, some parameters in the hybridization and culture methods were further optimized.

[0072] The operation method and precautions of this embodiment are the same as those of Embodiment 1, the difference is:

[0073] In step B, the hatching process is carried out in micro-flow water with a water temperature of 8-12°C under indoor shading conditions, with a DO value of 5-12 mg / l and a pH value of 6.5-7.5;

[0074] C. After the juvenile fish floated up, they were cultivated in separate ponds to obtain juvenile fish; in the cultivation, the culture density was 1000 tails / m2;

[0075] D. When the juveniles grow to a length of 8-12 cm, they are moved to an outdoor culture pond for 2-3 years to sexual maturity to obtain hybrid offspring;

[0076] The number of rainbow trout described in this embodiment is 10 tails, the number of arctic salmon is 3 tail...

Embodiment 3

[0116] This embodiment is a preferred solution based on Embodiment 1. In this example, some parameters in the hybridization and culture methods were further optimized.

[0117] The operation method and precautions of this embodiment are the same as those of Embodiment 1, the difference is:

[0118] In step B, the incubation process is carried out in micro-flow water with a water temperature of 10°C under indoor shading conditions, with a DO value of 5 mg / l and a pH value of 7.5;

[0119] C. After the juvenile fish floated up, they were cultivated in separate ponds to obtain juvenile fish; in the cultivation, the culture density was 1000 tails / m2;

[0120] D. When the juveniles grow to a length of 8-12 cm, they are moved to an outdoor culture pond for 2-3 years to sexual maturity to obtain hybrid offspring;

[0121] E. Detecting the hybrid progeny to obtain the fertile tetraploid rainbow trout;

[0122] The detection method is described in Example...

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Abstract

The invention provides a rainbow trout tetraploid breeding method. Viable tetraploid rainbow trout is obtained in the method by using generic cross of rainbow trout and arcticchar. The rainbow trout and the arcticchar belong to oncorhynchus and salmo respectively; the rainbow trout is sexually matured female; and the arcticchar is sexually matured male. The generic cross of the rainbow trout and the arcticchar is used, fertilized eggs are hatched, young fish is bred and cultured for 2 to 3 years till sexual maturity; partial viable tetraploid exists in the filial generation by detection; and the method provides a feasible path for producing triploid rainbow trout in large scale. Reasonable parameters in the hatching and breeding steps are favorable for growth of larval fish and juvenile fish; the detection method combines cytology detection and chromosome detection of the microscopic level and gonad development condition detection of the macroscopic level, is strict and ordered, and is favorable for accurately judging whether the tetraploid filial generation is generated; and each operation method is simple and feasible, and has good suitability.

Description

technical field [0001] The invention belongs to the field of artificial propagation of fish, and in particular relates to a tetraploid breeding method for rainbow trout. In the method, fertile tetraploid rainbow trout is obtained by performing intergeneric hybridization with rainbow trout and char. [0002] Background technique [0003] In the study of polyploidy in fish, Svardson first discovered polyploid types in Salmonidae in 1945. So far, polyploid fish have been successfully induced in more than 40 species of bony fishes in 11 orders and 25 families. The breeding research of fish polyploids, mainly triploids and tetraploids, has been highly valued by people because of their broad application prospects. For example, some sterile triploid fish have fast growth speed, meat quality It has the advantages of good quality, strong stress resistance, and no interference with fish resources. It is an economic fish with promotion prospects. At present, there are biological, p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 白宝海
Owner 北京卧佛山庄养殖有限公司
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