Mass spectrometry identification method for salmo salar and rainbow trout

Abstract

The invention discloses a mass spectrometry identification method for salmo salar and rainbow trout. The method includes the following steps: a) obtaining the amino acid sequence of salmo salar; b) obtaining the amino acid sequence of rainbow trout; c) utilizing a specific enzymatic method to cut to-be-detected protein into a small molecular weight polypeptide fragment mixture, utilizing an electrostatic field orbitrap high resolution mass spectrometry full scan mode to detect the molecular weight and fragment information of polypeptide in the mixture, performing comparison with an amino acidsequence obtained before, and qualitatively judging whether the characteristic peptide fragments of the salmo salar and rainbow trout exist; and d) selecting the characteristic peptide fragment determined as the salmo salar or the rainbow trout, and utilizing tandem quadrupole mass spectrometry to establish a multi-reaction monitoring method to perform quantitative analysis. Identification can beperformed by utilizing the protein characteristic peptide fragments, so that the qualitative identification and adulteration quantification of the salmo salar and rainbow trout can be simultaneously realized; and minimum detection limit can reach 1%, so that the method is high in sensitivity, high in accuracy and strong in anti-interference ability, and can be widely applied to the identificationof the salmo salar.

Description

technical field [0001] The invention relates to a protein mass spectrometry detection method, in particular to a mass spectrometry identification method for Atlantic salmon and rainbow trout. Background technique [0002] The authenticity and source assessment of seafood is an important part of food evaluation and identification. Many fish products are mislabeled, and even low-quality fish and counterfeit fish are labeled as high-quality fish, which seriously affects market transparency and damages the health of consumers. A few days ago, the member units of the China Aquatic Products Circulation and Processing Association and the Salmon Branch jointly passed the identification standards of salmon, and designated salmon as a collective name for salmon family fish. Atlantic salmon and rainbow trout are both a type of salmon. This has aroused widespread concern in the industry and doubts from consumers. [0003] Although Atlantic salmon and rainbow trout belong to the family...

AI Technical Summary

Problems solved by technology

The reagents used in PCR-RFLP and AFLP are harmful to the human body, and the operation steps are extremely cumbersome and time-consuming

However, although the identification of species by fluorescent real-time quantitative PCR is fast and accurate, the cost is relatively high.

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