The invention relates to a flowing chamber for a biofluid experiment. The technical scheme of the lowing chamber for that biofluid experiment is that the flowing chamber comprises an upper piece, a lower piece, an upper piece groove sealing washer, a cellular glass slide, a flowing chamber sealing gasket and a glass sealing piece, wherein the upper piece groove sealing washer, the cellular glass slide, the flowing chamber sealing gasket and the glass sealing piece are arranged sequentially from the upper piece to the lower piece, horizontal planes of the upper piece groove sealing washer, the cellular glass slide, the flowing chamber sealing gasket and the glass sealing piece are rectangular, the length and width dimensions of the upper piece groove sealing washer, the cellular glass slide, the flowing chamber sealing gasket and the glass sealing piece are same, and the upper piece and the lower piece are connected through bolts; the flowing chamber for the biofluid experiment has the advantages that the structure is simple, and the use is convenient; at the same time, the practicability is high, the size of the flowing chamber can be changed by simply adjusting the thickness of the flowing chamber sealing gasket, accordingly, a flexible effect is realized, and various experiment requirements are met; particularly, several stages of buffering flow balancing structures are designed at an inlet and an outlet of the flowing chamber, and in and out of fluid both pass through all stages of buffering flow balancing so that the experiment data are more accurate, and the experiment effect is better.