Method for using flow chamber for biological fluid experiment

Abstract

A using method of a flow chamber for a bio-fluid experiment comprises steps as follows: Step one, assembly: an upper sheet groove of an upper sheet is aligned to a top boss of a lower sheet for embodiment, and fixed nuts of fixing screw holes are sequentially mounted; Step two, feeding of a circulating fluid: two thin fluid tubes on one side of the flow chamber are butt-jointed through a semi-circular, upward and rainbow-shaped hose, one of the two thin fluid tubes on the other side is connected with a sample feeding hose, the other one is connected with a sample discharging hose, when the flow chamber is full of the fluid, the closed sample discharging hose is opened, the flow velocity is kept, a microscope lens is used for performing observation from the top, and the experiment is started; Step three, phase-change counter flow of the circulating fluid. The using method has the benefits as follows: the using method of the flow chamber for the bio-fluid experiment is convenient to use, and the flow chamber structure for the bio-fluid experiment is combined and formed quickly and efficiently through combination of the upper and lower sheets as well as a series of simple parts arranged between the upper and lower sheets.

Description

technical field [0001] The invention relates to a method for using a biomedical experiment device, in particular to a method for using a flow chamber for a biofluid experiment. Background technique [0002] A flow chamber for biological fluid experiments is an experimental component with a flow chamber that can be observed through a microscope. In the experimental research of biomedical engineering, for various cells such as vascular endothelial cells and blood cells, under the action of fluid force, the experimental research on cell proliferation and adhesion has been carried out in depth; however, due to the particularity of vascular structure , and the focus of the research on fluid mechanics and blood vessels is different, the above-mentioned experiments all need to design personalized experimental instruments; and because of the strong personalization of this research, there are almost no general-purpose vascular bionic cell fluid experimental instruments on the market;...

AI Technical Summary

Problems solved by technology

In the experimental research of biomedical engineering, for various cells such as vascular endothelial cells and blood cells, under the action of fluid force, the experimental research on cell proliferation and adhesion has been carried out in depth; however, due to the particularity of vascular structure , and the focus of the research on fluid mechanics and blood vessels is different, the above-mentioned experiments all need to design personalized experimental instruments; and because of the strong personalization of this research, there are almost no general-purpose vascular bionic cell fluid experimental instruments on the market; most of the research Mechanisms are all experimental instruments designed by themselves according to their own experimental needs; the flow chamber for biological fluid experiments is the core accessory of the experimental platform in fluid experimental instruments; it directly determines whether the experiment is successful or not, the experimental design, and the rationality of parameters. It is judged that the direct acquisition of experimental cell images and experimental data has played a crucial role; moreover, for the different sizes of the experimental platform, the requirements for the liquid circulation and flow rate required for the hydrodynamic experiment, the required flow chamber flow The size of the chamber is different, so it is necessary to specially design a flow chamber for biological fluid experiments to meet the experimental requirements; and it is matched with a specially designed cell fluid experiment instrument for cell fluid mechanics experiments, which makes the cost of each experiment higher. The specially designed flow chamber and supporting instruments are not universal, and the use efficiency is low; in short, the method of using the flow chamber for biological fluid experiments in the prior art has a single method and cannot be flexibly adjusted for different experimental conditions.

Images