High performance liquid chromatography for determinating taurine content based on differential detector

A technology of high-performance liquid chromatography and differential detector, applied in the field of high-performance liquid chromatography of taurine content, can solve the problems of cumbersome operation, high cost, complicated instrument operation, etc., achieve simple detector parameter setting and ensure quality control , the effect of a wide linear range

Inactive Publication Date: 2018-09-04
北京市东城区食品药品安全监控中心
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

Potentiometric titration is usually suitable for the determination of API samples that do not contain interfering substances or have few impurities. The spectrophotometric method after the color reaction of acetylacetone also requires a color reaction, and the operation is cumbersome. It is difficult to have an intuitive view of the reaction process and reaction conversion rate. The judgment of taurine will affect the accuracy of taurine determination; the principle of high performance liquid chromatography-evaporative light scattering detection method is to atomize the column eluent to form an aerosol, and then evaporate the solvent in the heated drift tube, and finally the remaining The non-volatile solute particles are detected in the light scattering detection cell, which requires high-pressure nitrogen or air as the carrier gas, which is inconvenient to use, the stability of the carrier gas, the atomization process, and the thermal stability of the sample to be tested will all affect the accuracy of the measurement; mass spectrometry The method has strong specificity and high accuracy, but it is expensive and the operation of the instrument is complicated. It is often used for the qualitative or quantitative determination of substance purity, structure or unknown samples, but it is used for routine testing of conventional known samples, and the cost of instrument use and test time Relatively high, which is not conducive to the saving of inspection costs and the improvement of inspection efficiency in conventional laboratories

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  • High performance liquid chromatography for determinating taurine content based on differential detector
  • High performance liquid chromatography for determinating taurine content based on differential detector
  • High performance liquid chromatography for determinating taurine content based on differential detector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This embodiment is based on the method of measuring taurine content based on the refractive index detector method, including the following steps:

[0037] (1) Precisely weigh about 10 mg of the taurine reference substance, put it in a 25mL measuring flask, add a 40:60 volume ratio of water-acetonitrile solution to dissolve and dilute to the mark, shake well to prepare a taurine reference substance solution;

[0038] Accurately weigh 10 mg of alanine reference substance, put it in a 25mL measuring flask, add a water-acetonitrile solution with a volume ratio of 40:60 to dissolve and dilute to the mark, shake well to obtain alanine reference substance solution;

[0039] (2) Precisely weigh an appropriate amount of fine taurine particles (equivalent to 100mg of taurine), put it in a 50mL measuring flask, add a water-acetonitrile solution with a volume ratio of 40:60 to dissolve and dilute to the mark, shake well, and filter; Precisely measure 5 mL of the continuous filtrate, put i...

Embodiment 2

[0055] Example 2 Investigation of linear relationship

[0056] Using the mobile phase as the solvent, take the concentration of 1mg / mL, 0.8mg / mL, 0.6mg / mL, 0.4mg / mL, 0.2mg / mL, 0.1mg / mL and 0.05mg / mL taurine reference solution respectively.

[0057] According to the chromatographic analysis conditions in Example 1, linear regression was performed with the peak area on the concentration of taurine reference substance (mg / mL) to obtain the regression equation Y=195768.56X+1085.93, r=0.99858 (n=7). The results show that the linear relationship is good within the range of injection concentration 0.05mg / mL-1mg / mL.

Embodiment 3

[0058] Example 3 Precision Experiment

[0059] Take 1mg / mL, 0.4mg / mL and 0.05mg / mL taurine reference solution respectively, according to the chromatographic analysis conditions in Example 1, repeat the injection 5 times, and the RSD of the peak area is 0.31% and 0.37 respectively. %, 0.36%.

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Abstract

The invention belongs to the technical field of medicine analysis, and particularly relates to a high performance liquid chromatography for determinating the taurine content of taurine granules or taurine eye drops based on a differential detector. According to the high performance liquid chromatography for determinating the taurine content based on the differential detector, high performance liquid chromatography is combined with a differential refraction detector to establish a high performance liquid chromatography-differential detection method for directly determining the taurine content;with an NH2 column as a separation column, detection is performed by using a differential refraction display; in the whole detection process, use of a carrier gas is not required and derivatization oftaurine is not required; in addition, no intermediate reaction process exists, the linear range is wide, and the taurine content can be directly determined easily, conveniently, quickly and accurately; by the high performance liquid chromatography, determination of the taurine content of the taurine granules and the taurine eye drops can be achieved.

Description

Technical field [0001] The invention belongs to the technical field of pharmaceutical analysis, and specifically relates to a high performance liquid chromatography method for measuring the taurine content in taurine particles or taurine eye drops based on a refractive index detector. Background technique [0002] Taurine, also known as β-aminoethanesulfonic acid, with the molecular formula C 2 H 7 NO 3 S, with a molecular weight of 125.15, is a sulfur-containing non-protein amino acid that was first isolated from bezoar. Taurine has a small molecule, does not have a phenyl group, has stable chemical properties, and is insoluble in organic solvents such as ether. It exists in a free state in the body and does not participate in the biosynthesis of proteins in the body. However, it interacts with cystine and cysteine. The metabolism of amino acid is closely related and is an important nutrient for humans and animals. It exists in almost all organs of mammals in free form, and it ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/047G01N2030/065
Inventor 王丽霞仵淑红
Owner 北京市东城区食品药品安全监控中心
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