Genitourinary tract mycoplasma culture medium and detect method thereof
A technique for urogenital tract and detection method, which is applied in the field of urogenital tract microorganism culture and detection, can solve the problems of no mycoplasma culture medium and preparation method, the culture medium affects the detection effect, the culture medium is expensive, and the like, and achieves typical colony morphology. , the effect of prolonging the logarithmic phase of growth and reducing the false negative rate
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Embodiment 1
[0067] Culture medium configuration: Take a 2000ml clean small-bore, high-pressure resistant glass container with a cover (pluggable), weigh 5g~10g of trypsin soybean broth dry powder and dissolve it in 600ml of purified water, add NaCl and diphosphate Potassium hydrogen, adjust to pH 6.1~6.3 with 1N HCL, paste disinfection and sterilization instruction strips, place in a high-pressure container, sterilize at 121°C for 15 minutes after removing cold air, and remove residual steam after the pressure returns to zero. Cover and cool naturally for later use.
[0068] Take a clean container and add an appropriate amount of sterilized purified water, weigh yeast extract, urea, arginine, L-cysteine, and phenol red into the container one by one according to the formula, pressurize and pass through a 0.22μm filter , add the solution to the cooled above-mentioned culture medium, take a sterile container and inject an appropriate amount of sterilized water, weigh vancomycin, amphotericin...
Embodiment 2
[0071] The configuration of the solid medium: take a 2000ml clean small-caliber, high-pressure-resistant thick-walled glass container with a cover (can be plugged), add about 500ml of purified water, and weigh trypsin soybean broth dry powder, agar powder, Dissolve manganese sulfate into the container one by one, adjust to pH 6.1~6.3 with 1N HCL, paste the disinfection and sterilization instruction strip, put it in a high-pressure container, and sterilize at 121°C for 15 minutes after excluding cold air, and wait until the pressure returns to zero Finally, remove the residual steam and open the cover to cool naturally to 56°C, and keep it warm for later use.
[0072] Take a clean container and add an appropriate amount of sterilized purified water, weigh the yeast extract, urea, arginine, L-cysteine, and phenol red into the container one by one to dissolve, pass through a 0.22 μm filter, and then pour into the insulated culture In the base, take a sterile container and inject ...
Embodiment 3
[0075] Comparison of the detection ability of different culture media on clinical samples: take swabs of clinical samples, put them into sterile small test tubes containing 50 μl to 200 μl of normal saline, discard the swabs after fully stirring, and add the liquid samples equally to the present invention , imported A and domestic B liquid medium, at 37 ℃, 5% CO 2 Cultivate under the conditions for 24 hours, and replant in the solid medium of the present invention and import A respectively, because domestic B commodity does not have corresponding solid medium, only compare the performance of liquid medium. Liquid and solid media containing samples to be tested, continue to store at 37°C, 5% CO 2 conditions, up to 72 hours. Observe the following indicators of the medium every 12 hours: whether the liquid medium changes color, time, turbidity, solid culture colony growth rate, in order to understand the positive rate of culture, pH buffer capacity, record the changes in the thr...
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