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Polymyxin E separation preparation method

A technology for polymyxin and fermentation broth, applied in the field of separation and preparation of polymyxin E, can solve the problems of high cost, pollution, low yield and the like, achieve broad economic effects, reduce pollution, and simplify the effect of extraction process

Inactive Publication Date: 2006-07-12
HEBEI UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of high cost, low yield and serious pollution of polymyxin E in the traditional separation of fermentation broth. After long-term research and experiments, it is proposed to use foam separation technology to separate polymyxin E from fermentation broth. A new separation method of mycolistin E, thereby overcoming the disadvantages of the prior art, and obtaining the polymyxin E raw material drug

Method used

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  • Polymyxin E separation preparation method

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Effect test

Embodiment 1

[0022] a. Pretreatment of fermentation broth:

[0023] The fermented liquid containing polymyxin E produced by Bacillus Pollus Polymyxa Var Colistinus (the fermented liquid was purchased from Schreck (Tianjin) Biotechnology Co., Ltd., the same as the following examples) was used in oxalic acid solution Adjust the pH value to 3.5, heat at 80°C for 30 minutes, and filter the acidified fermentation broth through a plate frame for 2.5 hours. The yield of polymyxin E was 96%.

[0024] b. Foam separation

[0025] Use a bubble column for batch separation at a temperature of 50°C, a pH of 4.5, and a superficial gas velocity of 1×10 -3 m / s, the foam breaking method adopts a mechanical method, and the foam is broken by stirring. The operation time of one cycle is 2.5 hours, and a high-concentration polymyxin E foam breaking liquid is obtained, and its concentration factor is 14. The yield of polymyxin E was 90%.

[0026] c. Vacuum evaporation

[0027] The foam breaking solution was...

Embodiment 2

[0031] a. Pretreatment of fermentation broth:

[0032] The fermentation broth containing polymyxin E produced by Bacillus polymyxa colistin variants is adjusted to pH 2.5 with oxalic acid solution, heated at 90°C for 20 minutes, and the acidified fermentation broth is filtered through a plate frame for 2 hours . The yield of polymyxin E was 94%.

[0033] b. Foam separation

[0034] Use a bubble column for batch separation at a temperature of 30°C, a pH of 3.5, and a superficial gas velocity of 1×10 -4 m / s, the foam-breaking method adopts the method of adding an organic solvent, adds dehydrated alcohol by the spray flow of 2% of the foam-breaking liquid amount, and a cycle operation time is 3.0 hours to obtain a high-concentration polymyxin E foam-breaking liquid, which The concentration factor is 13. The yield of polymyxin E was 90%.

[0035] c. Vacuum evaporation

[0036] The foam breaking solution was evaporated in vacuo. The pH is 5.0, the temperature is 80°C, and th...

Embodiment 3

[0040] In the foam separation step in b, the foam breaking method adopts the method of adding an organic solvent, and acetone is added to the 1% spray flow of the foam breaking liquid amount, and other steps are the same as in Example 2. Step b obtains a high-concentration polymyxin E foam-breaking liquid, and its concentration factor is 14. The yield of polymyxin E was 92%.

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Abstract

The invention provides a method for separately preparing for polymyxin E, which uses foam separation method to extract and separate the polymyxin E from fermentation liquor. The fermentation liquor obtains polymyxin E raw medicine by first treatment, form separating, vacuum evaporation and spray drying.

Description

technical field [0001] The invention relates to the extraction, separation and purification of polypeptide antibiotics, in particular to a method for the separation and preparation of polymyxin E. Background technique [0002] Polymyxin E (Polymyxin E) is a basic polypeptide antibiotic produced by Bacillus Pollus PolymyxaVar Colistinus, which has a strong bactericidal effect on Gram-negative bacilli and can treat a variety of Animal feeding diseases caused by germs have good antibacterial spectrum, high efficiency, and low toxicity. They have been used as feed additives, and can promote animal biology and improve feed utilization. [0003] At present, the separation of polymyxin E from the fermentation broth adopts the resin adsorption analysis method, which has long steps, high energy consumption and high cost for separating polymyxin E in the fermentation broth. Moreover, the resin adsorption analysis method brings a large amount of sewage and seriously pollutes the envir...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K14/195
Inventor 吴兆亮佟斌周丽亚郑辉杰
Owner HEBEI UNIV OF TECH
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