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Endotoxin adsorptive material, preparing and use thereof

An adsorption material and endotoxin technology, applied in the field of biomedical materials, can solve the problems that cannot be used to treat endotoxemia, poisoning, and intravenous injection

Inactive Publication Date: 2005-02-23
浙江科锐生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because polymyxin has a severe toxic effect on the human nervous system and kidneys, it can only be administered orally and locally injected at present, and cannot be administered intravenously at present, so it cannot be used for the treatment of endotoxemia

Method used

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  • Endotoxin adsorptive material, preparing and use thereof
  • Endotoxin adsorptive material, preparing and use thereof
  • Endotoxin adsorptive material, preparing and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the preparation method of agarose gel beads-polymyxin endotoxin adsorbent

[0022] Dried Sepharose CL-4B 50mL, washed 3 times with 3 liters of 1M deoxycholic acid, washed 3 times with 3 liters of sterile pyrogen-free water, pH 7.0, and drained. Use the following two methods (1) and (2) to activate and immobilize:

[0023] (1) Soak in 200mL epichlorohydrin (0.1M sodium hydroxide solution), shake and react at 45°C for 5 hours, rinse with 3 liters of sterile pyrogen-free water for injection to pH 7.0, filter and drain. Add 100 mL of ammonia water, shake and react at 45°C for 5 hours, add 5 mL of 25% glutaraldehyde, react at 45°C for 2 hours, wash with sterile pyrogen-free water, filter and drain. Add 200 mL of solution A (350 mg / mL polymyxin, 0.5 M sodium chloride, 0.1 M sodium bicarbonate), and react at 20° C. overnight. 10 mL of 1M glycine and 2 g of sodium borohydride were added, and the reaction was continued for 2 hours. Filter and drain, 3 liters of ...

Embodiment 2

[0027] Embodiment 2: the preparation method of PVA-polymyxin endotoxin adsorbent

[0028] Dried PVA 50mL, washed 3 times with 3 liters of 1M deoxycholic acid, washed 3 times with 3 liters of sterile pyrogen-free water, pH 7.0, and drained. Use the following two methods (1) and (2) to activate and immobilize:

[0029] (1) Soak in 200mL epichlorohydrin (0.1M sodium hydroxide solution), shake and react at 55°C for 2 hours, rinse with 3 liters of sterile pyrogen-free water for injection to pH 7.0, filter and drain. Add 100 mL of ammonia water, shake and react at 55°C for 2 hours, add 5 mL of 25% glutaraldehyde, react at 55°C for 2 hours, rinse with sterile pyrogen-free water, filter and drain. Add 200 mL of solution A (350 mg / mL polymyxin, 0.5 M sodium chloride, 0.1 M sodium bicarbonate) and react at 35° C. for 6 hours. 10 mL of 1M glycine and 2 g of sodium borohydride were added, and the reaction was continued for 1 hour. Filter and drain, 3 liters of sterile pyrogen-free wate...

Embodiment 3

[0040] Embodiment 3: the use method of endotoxin adsorption material:

[0041] (1) Mammalian whole blood perfusion:

[0042] Material 4 was packed into a column (inner diameter 4 cm, height 4 cm) for hemoperfusion.

[0043] The femoral artery and femoral vein of the mixed adult dog (weight 7-9kg) were connected to the perfusion device through a tube pump system, the blood flow rate was controlled at 50mL / min, and the dog was under anesthesia. 15 minutes after the start of perfusion, the normal saline containing E. coli 0111:B4 0.01% (0.5mg / kg body weight) lipopolysaccharide solution was titrated for 60 minutes and injected into the forefoot, and the amount of heparin in the perfusion test was controlled at 100 units / kg body weight / hour , perfusion continued for 2 hours. One in 10 dogs died after 24 hours (mortality 10%), compared to 7 and 9 deaths in the control experiments of Sepharose CL-4B and PVAC treated dogs respectively (mortality 70% and 90%). The other 5 dogs that ...

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Abstract

An endotoxic adsorpting method for treating endotoxemia and anaphylactic reaction caused by endotoxemia is prepared through activating the hydroxy group of agarose gel or DVA as carrier by cyanogen bromide or epoxy chloropropane or sulfuryl chloride, and adding polymyxin solution.

Description

Technical field: [0001] The invention relates to the field of biomedical materials, in particular to an affinity adsorption material capable of adsorbing and removing endotoxin molecules in a liquid mobile phase and a preparation and use method thereof. Background technique: [0002] Endotoxin is a lipopolysaccharide molecule from the cell wall of Gram-negative bacteria. Endotoxin enters the blood of mammals and can cause fever. When the amount of endotoxin in the blood reaches a certain limit, it can directly cause death. Escherichia coli (a typical Gram-negative bacterium) often appears in the intestinal tract of these animals, but the general toxicity will disappear after being absorbed by the liver, and Escherichia coli and endotoxin will not be found in normal human blood. However, when the patient's liver function declines, he receives anticancer drug treatment, or suffers from severe trauma (burn or surgery), he is prone to severe bacterial infectious diseases, follow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61M1/00B01J20/22
Inventor 肖海军
Owner 浙江科锐生物科技有限公司
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