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A culture medium and method for culturing micro vibrio cholerae therewith

A technology of culture medium and sodium dodecyl sulfate, applied in the field of culture medium, can solve the problems that positive cultures cannot be directly obtained, false positives and false negatives cannot be ruled out, and conventional methods cannot be replaced, so as to improve the response to cholera outbreaks The ability to prevent epidemics, save disease prevention funds, and facilitate quality control

Inactive Publication Date: 2006-09-27
刘捷 +2
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  • Summary
  • Abstract
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  • Claims
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Problems solved by technology

[0007] Most of the above methods have the advantages of fast, sensitive, and specific, but they are not easily accepted by the grassroots because of the cumbersome methods, equipment limitations, or inconvenient operation due to observation results, or because of high prices and high costs. The biggest disadvantage is that They can only be used as qualitative tests, and they cannot directly obtain positive cultures, and cannot exclude false positives and false negatives, so they cannot replace conventional methods. They are only listed as rapid auxiliary diagnostic methods in the cholera control manual.

Method used

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Embodiment Construction

[0046] A medium is made from the following components:

[0047] Peptone 10g, beef extract 2g, sodium chloride 5g, agar 20g, distilled water 1000ml, yeast extract 3g, anhydrous sodium sulfite 3g, sodium citrate 10g, sodium lauryl sulfate 0.5g, sucrose 10g, the percentage concentration is 0.4% 1ml of phenol red, 1ml of gentamicin (500μ / ml), 1ml of polymyxin B (1000μ / ml), 1ml of potassium tellurite with a percentage concentration of 1%, 1ml of promethazine, pH value 8.6.

[0048] Use above-mentioned substratum to carry out the microculture method of Vibrio cholerae, its step is:

[0049] 1. Weigh the raw materials according to the above composition formula, mix and heat to melt, and the heating temperature is 100°C;

[0050] 2. Add the heated liquid into the evenly distributed pits on the bacterial culture plate;

[0051] 3. After the liquid in the pit is solidified, pack the bacterial culture plate in a vacuum-sealed plastic bag, put it in the refrigerator, and store it at a t...

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Abstract

The invention discloses a culture medium and micro-culture method of cholera vibrio through this culture medium, wherein the culture medium comprises the following parts: peptone, beef grease, sodium chloride, agar, yeast leaching grease, anhydrous sodium sulfite, sodium citratej, dodecyl sodium sulfate, sucrose, phenol red, garamycin, polymyxin, potassium tellurite, benzalkonium bromide and distilled water; the method comprises het following steps: adding the mixed, melt and heat materials on the even pit of culture plate; putting the solidifying culture plate through plastic vacuum bag in the fridge; keeping under 2-6 deg.c; fetching the culture plate at the bacteria culture time; opening the package; seeding the detected sample in the pit; stewing for 9-18 h at 36+-1 deg.c; observing the result of culture plate; displaying yellow if there is growing bacteria; fitting for serum experiment directly.

Description

technical field [0001] The invention belongs to a vibrio cholerae culture medium and a vibrio cholerae culture method thereof, in particular to a culture medium and a micro culture method for cultivating vibrio cholerae with the culture medium. Background technique [0002] Cholera is an acute intestinal infectious disease caused by Vibrio cholerae of 01 serogroup and 0139 serogroup. Its onset is rapid, its spread is fast, and its spread is wide. If it is not treated in time, the fatality rate is high. It is an international infectious disease characterized by causing a pandemic. one. [0003] One of the main countermeasures for the prevention and control of cholera is to implement epidemic monitoring, diagnose and deal with the epidemic in time, and prevent the spread. The determination of the epidemic mainly depends on the correct diagnosis of the laboratory. The diagnostic principle of cholera laboratory is: based on serological traits, combined with morphological and c...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/63
Inventor 刘捷高涛张明理
Owner 刘捷
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