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Ketamine-collaurum test paper for detection of saliva

A technology of saliva detection and ketamine, which is applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as adulteration, cross-cutting, and false negatives, and achieve clear and easy-to-discern results, improved sensitivity, and simple operation.

Inactive Publication Date: 2012-08-15
戴国华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing urine test strips have the following deficiencies during their use (1) sample collection is unhygienic, which is difficult for many sample collectors and testers to accept; (2) privacy issues, that is, sexually concealed urine collection, Female personnel are required to supervise female urine tests; (3) for recruits, civil servants, and special practitioners (pilots, drivers, aerial workers, etc.) Need to have special place when urinating, and must carry out one-to-one supervision to take urine, very inconvenient, is not suitable for driver's roadside screening and recreational place sudden spot check; Crossover issues with drugs such as methorphan, resulting in false and false detections

Method used

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  • Ketamine-collaurum test paper for detection of saliva
  • Ketamine-collaurum test paper for detection of saliva
  • Ketamine-collaurum test paper for detection of saliva

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The preparation of embodiment 1 ketamine colloidal gold detection test strip (referring to Fig. 1)

[0050] 1. Preparation of colloidal gold:

[0051] Accurately measure 100mL of purified water, put it into a round bottom flask, heat and stir until boiling. Add 2mL of 1% chloroauric acid solution into boiling water, continue heating and stirring. When the solution boils again, accurately measure 2 mL of 1.0% trisodium citrate solution and quickly add it to the solution, and continue to boil. Carefully observe the color of the solution. When the solution turns from yellow to purple-black, and finally becomes stable to wine red, start timing and continue heating for 10 minutes. After the temperature of the solution returns to room temperature, stop stirring, restore the volume to 100 mL with purified water, and store at 4°C in the dark for future use.

[0052] 2. Preparation of ketamine monoclonal antibody gold label pad

[0053] 2.1 The required raw material ketamine...

Embodiment 2

[0073] The preparation of embodiment 2 ketamine colloidal gold detection test strips

[0074] 1. Preparation of colloidal gold:

[0075]Accurately measure 100mL of purified water, put it into a round bottom flask, heat and stir until boiling. Add 2mL of 1% chloroauric acid solution into boiling water, continue heating and stirring. When the solution boils again, accurately measure 2 mL of 1.0% trisodium citrate solution and quickly add it to the solution, and continue to boil. Carefully observe the color of the solution. When the solution turns from yellow to purple-black, and finally becomes stable to wine red, start timing and continue heating for 10 minutes. After the temperature of the solution returns to room temperature, stop stirring, restore the volume to 100 mL with purified water, and store at 4°C in the dark for future use.

[0076] 2. Preparation of ketamine monoclonal antibody gold label pad

[0077] 2.1 Required raw materials

[0078] Ketamine monoclonal ant...

Embodiment 3

[0093] Embodiment 3 detection method (referring to Fig. 2)

[0094] Spit or drop the saliva of the specimen to be tested directly into the test strip sample loading area or saliva collection device prepared in Example 1 and Example 2, the sample liquid moves forward along the membrane by capillary action, within 3-5 minutes Interpretation results, if more than 10 minutes, the results are invalid.

[0095] Result: If the sample contains ketamine (positive), a red band appears at the quality control line C, and there is no band at the reaction line T.

[0096] If there is no ketamine (negative) in the sample, there will be a band at the quality control line C and the reaction line T respectively, and there will be two bands in total.

[0097] The test results form a band at "C" regardless of whether ketamine is present in the specimen. This line is a quality control line. If the colloidal gold fails, this line will not appear, indicating that the test strip is invalid.

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Abstract

The invention provides ketamine-collaurum test paper for detection of saliva. The ketamine-collaurum test paper comprises a reaction film and a gold mark cushion, wherein according to the reaction film, a reaction line and a quality control line are formed by respectively coating ketamine and quality-control secondary antibodies IgG (immunoglobulin G) connected with carrier protein on a nitrocellulose film (NC film); the gold mark cushion is coated with ketamine monoclonal antibodies marked with collaurum; and a film chromatography competitive inhibition method is adopted to detect the ketamine in a saliva sample. The test paper can be used for effectively monitoring the ketamine in saliva, the detection specificity is strong, the repeatability is good, the sensitivity is high, and the lowest detection quantity reaches 50ng / ml. The test paper is simple to operate, does not need special instruments and equipment, does not need professional training and has clear and obvious results, thus being suitable for field detection.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to a ketamine colloidal gold saliva detection test strip. Background technique [0002] Ketamine (KET) is a derivative of N-1-phenylcyclohexy-piperidine (PCP). It is used as an anesthetic for surgical operations in medicine. It is commonly known as "K powder" and "K boy" in the black market. In recent years, the abuse in entertainment venues has become increasingly serious. It is one of the main new drugs in my country at present. At present, there are more than 120 kinds of narcotic drugs and more than 130 kinds of psychotropic drugs listed in my country. Studies have shown that after taking ketamine, there will be "depersonalization", "derealization", changes in body image, dreams, hallucinations, and nausea and vomiting. Taking large doses will cause confusion, body numbness, respiratory depression, and even Limb convulsions and respiratory arrest appear; long-term use of k...

Claims

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Application Information

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IPC IPC(8): G01N33/64G01N33/577G01N33/558
Inventor 戴国华
Owner 戴国华
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