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Colloidal gold immunochromatographic test strip for detecting shrimp allergen and preparation method thereof

A chromatography test paper and colloidal gold technology, applied in the field of immunological detection, can solve the problems of high cost, restricting the development of allergen detection methods, and slow detection speed, and achieve the effect of easy operation

Inactive Publication Date: 2010-11-10
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many methods for allergen detection at present, they all have different problems, such as slow detection speed, high cost, and the need for specific analytical instruments, etc., which restrict the development of allergen detection methods in food

Method used

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  • Colloidal gold immunochromatographic test strip for detecting shrimp allergen and preparation method thereof
  • Colloidal gold immunochromatographic test strip for detecting shrimp allergen and preparation method thereof
  • Colloidal gold immunochromatographic test strip for detecting shrimp allergen and preparation method thereof

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Embodiment 1

[0019] One end of the PVC backing is followed by a sample pad and a colloidal gold binding pad, a nitrocellulose membrane in the middle, and an absorbent pad on the other end. The binding pad is coated with anti-prawn tropomyosin cluster specific antibody-colloidal gold marker, nitrocellulose The film is coated with shrimp tropomyosin and goat anti-rabbit IgG.

[0020] Prepare according to the following steps:

[0021] (1) Extraction and purification of shrimp tropomyosin: homogenize the shrimp meat, immerse it in 0.01M PBS (pH 7.4) solution for 24 hours, precipitate with 50% saturated ammonium sulfate solution, and collect the precipitate after centrifugation at 8000g for 15 minutes at 4°C and dissolve in 0.01 MPBS (pH 7.4) solution, then dialysis in the PBS solution for 48h desalting, and finally gel filtration (column: Superdex 75 10 / 300 GL; buffer: pH 7.4, 0.01M PBS; flow rate: 0.35ml / min);

[0022] (2) Preparation of polyclonal antibodies against shrimp tropomyosin; immunize Ne...

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Abstract

The invention relates to a colloidal gold immunochromatographic test strip for detecting shrimp allergen and a preparation method thereof, belonging to the technical field of immunoassay. The invention discloses the assembled test strip which takes a combination of shrimp allergen-tropomyosin cluster specific antibody and color-developing substance colloidal gold as a detection reagent, the test strip is used for detecting the shrimp allergen in a food, and the test strip has low cost, fastness and portability. The applied antibody is an extracted and purified polyclonal antibody from a New Zealand rabbit with tropomyosin immune health. Due to the adoption of the polyclonal antibody, the test strip has low cost, good stability and good repeatability, and can be used in the detection of different samples.

Description

Technical field [0001] The invention relates to an immune colloidal gold chromatography test strip for detecting shrimp allergens and the preparation of the test strip, belonging to the technical field of immunological detection. Background technique [0002] In recent years, food allergy has become a public food safety issue, and shrimp is one of the eight most common food allergens. Allergic diseases seriously endanger people's health. Although the minimum amount of allergen that triggers an allergic reaction varies from person to person, a small amount of allergen can cause most patients to produce allergic symptoms. In order to avoid exposure to trace allergens, allergen testing has become a top priority. Now, although there are many allergen detection methods for reference, there are different problems in specific applications. In vivo experimental methods can provide the most direct evidence, but due to safety factors and other considerations, they can only be carried out...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/532
Inventor 胥传来邓小芳勇倩倩马文蔚陈莲君严文静屈昌龙吴晓玲
Owner JIANGNAN UNIV
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