Water body Chlamydomonas reinhaidtii toxin detection method

Abstract

The invention relates to a measuring method of micro-capsule alga toxin in water. The method includes the steps as follows: firstly, compounding and appraising of the micro-capsule alga toxin; secondly, preparing and purifying of polyclonal antibody antibody: immunizing the New-Zealand rabbit by making the micro-capsule alga toxin holoantigen MC-LR-KLH as the immunity resource, and preparing the polyclonal antibody and purifying by ammonium sulphate according to the normal method; thirdly, preparing immune bead: coupling the micro-capsule alga toxin holoantigen MC-LR-BSA and the nanometer bead, and preparing the immune bead containing MC-LR-BSA; fourthly, embedding the antibody to the pyroxylin film; fifthly, producing testing board, combing the coupling mat of the magnetic scale MC-LR-BSA, the pyroxylin film embedding the polyclonal antibody, the sample mat, the sopping mat, the covering film, the testing board out card into a testing board; sixthly, sample testing, respectively adding standard goods and testing samples with different concentrations into the sampling holds on the testing board, the samples flow on the test paper through the chromatography effect, after 3 to 5 minutes of the reaction under the room temperature, the testing board is put into a magnetic single testing apparatus to be tested, and the testing apparatus can further output the biology reaction signals being transferred to the magnetic field signals by the way of the electric signals; after the standard curve being drawn, the specific value of the micro-capsule alga toxin content in the sample to be tested is counted based on the standard curve.

Description

technical field [0001] The invention relates to a detection method capable of rapidly and sensitively detecting microcystin (MC) in water bodies, which is used for detecting the content of microcystins in water bodies and drinking water in natural environments. Background technique [0002] The increasingly serious water eutrophication has made water pollution a global environmental problem. Blue-green algae are the dominant algal species that form algae blooms in most freshwater lakes in China. These algae can produce peptides with obvious liver toxicity, called microbes. Cystin. Its toxicity ranks second among toxins known in nature, after dioxin. There are many different isomers of microcystins, among which MC-LR is the most toxic and acutely harmful freshwater cyanotoxin known so far. [0003] The detection methods of microcystins in water mainly include biological analysis, chemical analysis, biochemical analysis and immunoassay. The bioanalysis method is to evaluate...

AI Technical Summary

Problems solved by technology

The bioanalysis method is to evaluate the toxicity of microcystins by intraperitoneal injection or oral gavage in mice. This method is simple to operate, but the sensitivity is poor, and the type and structure of the toxin cannot be determined; the chemical analysis method is the most widely used method at present. Including Gas Chromatography (GC), Thin Layer Chromatography (TLC), High Performance Liquid Chromatography (HPLC), Liquid Chromatography / Mass Spectrometry (LC / MS) and Capillary Electrophoresis (CE), etc. These methods have good sensitivity and selection However, the instrument is bulky, expensive, requires indoor environment with high environmental conditions, and specialized technical personnel to operate, and the detection cost is expensive; biochemical analysis mainly includes enzyme activity inhibition detection technology, the principle of which is to use microcystin The degree of inhibition of protein phosphatase activity is detected by a colorimetric method, which is too sensitive and interferes with endogenous enzymes

Immunoassay is the preferred method for detection of microcystins by the U.S. Environmental Protection Agency. The most commonly used method is the competitive heterogeneous enzyme-linked immunoassay. , the results cannot be tested on-site

Images