Nontoxic ST28 streptococcus suis and application thereof
A technology of Streptococcus suis and strains, applied in the direction of bacteria, antibacterial drugs, bacterial antigen components, etc., can solve the problems of small inoculation dose, strong virulence, complex antigen components, etc.
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[0023] PCR Mix (Guangzhou Dongsheng Biotechnology Co., Ltd.)
[0024] DNA gel recovery kit (Axygen)
[0025] Streptococcus suis type 2 virulent strain ZY05719 (Shen Yan, Hua Xiuguo, Cui Li, Zhu Jianguo, Yang Zhibiao, Effects of two representative strains of Streptococcus suis type 2 infection on some immune indicators in miniature pigs, Journal of Animal Husbandry and Veterinary Medicine 2008, 39(12): 1721-1730, isolated from a diseased pig in Ziyang, Sichuan, donated by the preventive veterinary microbiome of Nanjing Agricultural University)
[0026] Bacto TM Todd Hewitt Broth (THB) Medium (BD Company)
[0027] THB liquid medium: Weigh 30g THB, dissolve in 1000mL deionized water, autoclave at 121°C for 20 minutes
[0028] THB solid medium: add 15g agar powder per 1000mL THB, autoclave at 121°C for 20 minutes, pour an appropriate amount into a plate to solidify
[0029] (1) The present invention isolates and obtains a non-toxic ST28 Streptococcus suis from healthy slaught...
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