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Nontoxic ST28 streptococcus suis and application thereof

A technology of Streptococcus suis and strains, applied in the direction of bacteria, antibacterial drugs, bacterial antigen components, etc., can solve the problems of small inoculation dose, strong virulence, complex antigen components, etc.

Active Publication Date: 2013-01-16
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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AI Technical Summary

Problems solved by technology

[0003] According to the concept of "prevention instead of treatment" of animal epidemics, immunization vaccines are the key to the prevention and control of streptococcal suis. Traditional vaccines include inactivated vaccines and live attenuated vaccines. The advantages of inactivated bacterial vaccines and inactivated vaccines are that they are easy to produce, stable, and easy to store. The disadvantages are that multiple immunizations are required, the immunization dose is large, and the immune site is damaged.
The advantage of the live attenuated vaccine is that it only needs to be vaccinated once, the dose is small, and the immune protection period is long. The disadvantage is that it is unstable, difficult to store, and the virulence may relapse
The above two types of vaccines are bacterial vaccines, and the antigen composition is relatively complex. In addition to the protective antigen, it may contain more ineffective antigens, and the existence of ineffective antigens will interfere with the body’s response to the protective antigens, and sometimes cause serious side effects

Method used

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  • Nontoxic ST28 streptococcus suis and application thereof
  • Nontoxic ST28 streptococcus suis and application thereof

Examples

Experimental program
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Embodiment Construction

[0023] PCR Mix (Guangzhou Dongsheng Biotechnology Co., Ltd.)

[0024] DNA gel recovery kit (Axygen)

[0025] Streptococcus suis type 2 virulent strain ZY05719 (Shen Yan, Hua Xiuguo, Cui Li, Zhu Jianguo, Yang Zhibiao, Effects of two representative strains of Streptococcus suis type 2 infection on some immune indicators in miniature pigs, Journal of Animal Husbandry and Veterinary Medicine 2008, 39(12): 1721-1730, isolated from a diseased pig in Ziyang, Sichuan, donated by the preventive veterinary microbiome of Nanjing Agricultural University)

[0026] Bacto TM Todd Hewitt Broth (THB) Medium (BD Company)

[0027] THB liquid medium: Weigh 30g THB, dissolve in 1000mL deionized water, autoclave at 121°C for 20 minutes

[0028] THB solid medium: add 15g agar powder per 1000mL THB, autoclave at 121°C for 20 minutes, pour an appropriate amount into a plate to solidify

[0029] (1) The present invention isolates and obtains a non-toxic ST28 Streptococcus suis from healthy slaught...

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Abstract

The invention relates to a nontoxic ST28 streptococcus suis and an application thereof, and belongs to the biotechnology field. A tonsil sample of a healthy slaughter pig is collected; after bacteria enrichment processing, bacteria DNA (deoxyribonucleic acid) is extracted; a cps2j gene is identified by polymerase chain reaction; then, pure culture bacteria can be obtained by bacteria plate separation; the pure culture bacteria is subjected to gram stain, serum agglutination, polymerase chain reaction and multilocus sequence typing to determine and separate type-2 bacterial strain of the streptococcus suis and a sequence type thereof; animal experiments of a Balb / c mice, a New Zealand rabbit and a Landrace piglet prove that the bacteria HA0609 is a nontoxic bacterial strain; after the bacteria is subjected to intramuscular injection into the New Zealand rabbit for one time and is subjected to intraperitoneal injection into a CD1 mice for two times, certain immunocompetence can be provided for an immunized animal, and analysis on a HA0609 immune protection antigen can be used for developing streptococcus suis disease subunit vaccine.

Description

technical field [0001] The present invention relates to a non-toxic ST28 Streptococcus suis and application thereof, specifically a non-toxic ST28 Streptococcus suis ( Streptococcus suis ), and the application of the bacterium in the development of Streptococcus suis vaccine belong to the field of biotechnology. Background technique [0002] Streptococcus suis type 2 ( Streptococcus suis serotype 2, S. suis 2) It is an important zoonotic pathogen, which can cause meningitis, sepsis, arthritis and other symptoms in pigs, and the fatality rate is as high as 40%. If it is complicated with porcine respiratory and reproductive syndrome, the mortality rate is higher, even if the disease The pigs that can survive after that grow slowly, and the feed-to-meat ratio increases, which brings great economic losses to the pig industry, so the bacteria is a potential threat to the global pig production. S. suis 2. It can infect people through wounds and digestive tracts. In 1998 an...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/09A61P31/04C12R1/46
Inventor 周俊明何孔旺虞凤倪艳秀吕立新张雪寒祝昊丹俞正玉茅爱华温立斌李彬郭容利王小敏汪伟周萍沈江萍
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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