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A strain of Pseudomonas fluorescens sc3 and its application in the control of bacterial soft rot in crops

A Pseudomonas fluorescens, bacterial technology, applied in the field of Pseudomonas fluorescens SC3 and its application in the control of bacterial soft rot in crops, can solve the problems of field trials, bacterial resistance, and water pollution and other problems, to achieve the effect of reducing the occurrence of bacterial soft rot in crops, good inhibitory effect, and good control effect

Active Publication Date: 2020-03-31
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, some studies have pointed out that Hydramycin and Junduqing can prevent and control bacterial soft rot diseases of bananas, but no field tests have been carried out.
However, chemical agents have certain toxicity to humans and animals, and can also cause problems such as bacterial resistance and water pollution.

Method used

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  • A strain of Pseudomonas fluorescens sc3 and its application in the control of bacterial soft rot in crops
  • A strain of Pseudomonas fluorescens sc3 and its application in the control of bacterial soft rot in crops
  • A strain of Pseudomonas fluorescens sc3 and its application in the control of bacterial soft rot in crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Isolation and Screening of Pseudomonas SC3

[0037] 1. Strain isolation

[0038] Sample collection: In the rice fields of Sichuan Agricultural University, the plants with rotten bases of rice seedlings were pulled out, together with soil samples from the roots of the rice, stored in bags, and brought back to the laboratory for strain isolation.

[0039] Isolation of bacterial strains: Soil samples were isolated by dilution coating plate method. Specifically, after the collected soil samples were thoroughly mixed by hand, 4.0 g was weighed, soaked in 20 mL of sterile water and shaken, and incubated at 28°C for 20 min on a shaker at 180 rpm for gradient dilution. Take the dilution factor as 10 5 -10 9 100 µL of the diluted solution was spread on the LB plate, air-dried at room temperature, sealed, and incubated upside down in a 28°C incubator for 24 h. Pick a single colony grown on the LB plate, label it, place it in a 2 mL centrifuge tube containing 500 µL ...

Embodiment 2

[0041] Example 2 Identification of Pseudomonas SC3

[0042] 1. Morphological identification

[0043]Strain SC3 is Gram-negative; the colonies on LB nutrient medium are light yellow, round, slightly convex in the middle, smooth in surface and neat in edge; diffuse turbidity in liquid medium, liquefaction of gelatin, production of acid, gas, Produces oxidase, catalase, and no amylase.

[0044] 2. Molecular identification

[0045] In order to clarify the taxonomic status of the strain SC3 obtained in Example 1, we used the method of multilocus sequence analysis (Multilocus sequences analysis, MLSA) combined with phylogenetic analysis (Phylogenetic analysis) to identify the SC3 strain.

[0046] 16S rDNA sequence analysis is a molecular biological identification method for bacterial identification. The present invention analyzes the 16S rDNA sequence and combines the conservative housekeeping gene gyrB , wxya and wxya sequence, using MEGA 5.0 software to construct a phyloge...

Embodiment 3

[0048] Example 3 Determination of Pseudomonas SC3 Antibacterial Spectrum

[0049] 1. In order to better study the biocontrol potential of bacterial strain SC3 of the present invention, we have studied the bacteriostatic spectrum of this bacterium. The specific operation is as follows:

[0050] (1) To explore whether SC3 has the ability to inhibit the growth of other pathogenic bacteria. Use the method identical with embodiment 1 bacterial strain screening, change pathogenic bacterium MS2 into the equal amount of rice base rot pathogen EC1, banana bacterial soft rot pathogen MS2 and MS3, bacterial wilt pathogen EP1 (first Replace 15 mL of LB with 15 mL of TTC), Brassicaceae black rot fungus Xcc, rice bacterial blight Xoo, peach rot fungus Pantoea ananatis PP1, yellow skin rot pathogen Pantoea anthophila CL1, the rest of the steps remain unchanged.

[0051] (2) To explore whether SC3 has the ability to inhibit the growth of pathogenic fungi. The fungi tested included ca...

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Abstract

The invention discloses a strain of Pseudomonas fluorescens ( Pseudomonas fluorescens ) SC3 and its application in the control of bacterial soft rot in crops. The strain Pseudomonas fluorescens SC3 was deposited in the Guangdong Microbial Culture Collection Center on April 30, 2018, and the preservation number is GDMCC NO. 60367. The strain SC3 has a significant inhibitory effect on a variety of bacterial disease bacteria, especially on bacterial soft rot of crops, R. solanacearum and Xanthomonas, reducing the occurrence of bacterial soft rot in crops , and the control effect was better in the pot experiment. Moreover, the strain SC3 itself has no pathogenicity and can be used to develop biopesticides against bacterial diseases of crops, providing new ideas and new ideas for the use of biological control of bacterial soft rot in crops.

Description

technical field [0001] The invention belongs to the technical field of plant disease biocontrol. More specifically, it relates to a strain of Pseudomonas fluorescens SC3 and its application in controlling bacterial soft rot of crops. Background technique [0002] Bacterial diseases are plant diseases caused by bacterial infection, such as soft rot, bacterial wilt, etc., causing serious economic losses. Symptoms of bacterial diseases are wilting, rotting, withering, etc. In the later stage of the disease, in wet weather, bacterial mucus overflows from the diseased part, and there is an obvious foul smell, which is the characteristic of bacterial diseases. [0003] For example, D. cornella ( Dickeya zeae ), the fungus has a wide range of hosts. In addition to infecting monocotyledonous plants, it can also infect dicotyledonous plants at the same time. Grass, pineapple, potato, tobacco, Chinese cabbage, carrot, peanut, impatiens, chrysanthemum, etc. reported by D. zeae Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/27A01P1/00C12R1/39
CPCA01N63/00C12N1/205C12R2001/39
Inventor 周佳暖李洁玲胡明张炼辉
Owner SOUTH CHINA AGRI UNIV
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