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Verticillium lecanii for preventing and controlling fly type pests and use thereof

A technology for Verticillium leucoidans and pests, which is applied in the directions of application, pesticides, fungi, etc., and achieves the effects of not easy to produce drug resistance, simple cultivation and large spore production.

Inactive Publication Date: 2011-01-19
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The genetic stability is good, and no toxicity has been found to humans, livestock, poultry, and plants. This fungus has not been reported in the control of fly pests.

Method used

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  • Verticillium lecanii for preventing and controlling fly type pests and use thereof
  • Verticillium lecanii for preventing and controlling fly type pests and use thereof
  • Verticillium lecanii for preventing and controlling fly type pests and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Embodiment one, the isolation of pathogenic bacteria, identification

[0008] 1.1 Materials and methods

[0009] 1.1.1 Materials

[0010] The adults of Boettcheriscaperegrine (Diptera: Sarcophagidae) infected by an entomogenic fungus were collected in Kunming.

[0011] Potato dextrose agar medium (PDA): 200g potato + 17-20g agar + 17-20g glucose + 1000ml water.

[0012] Sterile operating conditions: All utensils and utensils are sterilized in a high-temperature autoclave (121°C, 30min), and inoculation and other operations are performed in an ultra-clean workbench.

[0013] Culture conditions: Culture in a 29°C light (12L:12D) incubator. After the colonies are formed, transfer to the PDA slant of the test tube, cultivate for 2 to 3 days, and transfer to a 4°C refrigerator for storage.

[0014] 1.1.2 Isolation and purification of pathogenic bacteria

[0015] Isolation The pathogenic bacteria were isolated from the dead bodies of the diseased R. Soak the dead body of...

Embodiment 2

[0024] Embodiment two, the biological characteristics of the purified strain of Verticillium lecanii

[0025] 2.1 Materials and methods

[0026] 2.1.1 For the test strains, select a plate that grows uniformly and vigorously after purification as the test strains. The hyphae were inoculated on PDA again, and cultivated in a constant temperature light (12L:12D) incubator at 29°C.

[0027] 2.1.2 Determination of colony growth rate and sporulation amount Take a plate of Verticillium lecanii cultivated in advance and punch holes with an 8mm hole puncher, inoculate it on a new medium, do three repetitions, and place at 29 Cultivate in a constant temperature and light (12D:12L) incubator at ℃, measure and record its diameter regularly every day, until the colony is overgrown with the culture medium. Use a hole puncher with a diameter of 8mm to take the bacterium cake at the same position of the culture medium, add 1% Tween-80 and 20ml sterile water, wash the spores to make a spore ...

Embodiment 3

[0037] Example 3. Indoor pathogenicity determination of purified strains of Verticillium lecanii to Sarcophagus spp., Lucilia sericata, Musca domestica, Tysofly, and Drosophila

[0038] 3.1 Materials and methods

[0039] 3.1.1 Source of tested insects

[0040]Select the same batch of 4-day-old, disease-free, and uniformly sized adult larvae, Lucilia sericata, housefly, casefly, and fruit fly as test insects, and put the test insects separately into small insect cages , fed with water and white sugar, and reared under the conditions of 29±1°C and a photoperiod of 12L:12D.

[0041] 3.1.2 Preparation of spore suspension

[0042] Take the well-growing purified Verticillium lecanii and wash the spores with sterile water and filter (generally, the number of spores in the filtrate can reach 10 8 spores / ml), use a sterile capillary pipette to take a drop of the filtrate and drop it on a hemocytometer, count the spores under a microscope and record the data, and then dilute to 10 wi...

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Abstract

The invention relates to an entomogenous fungus-Verticillium lecanii, which is particularly effective in preventing and treating familiar dipterous fly insects. A wild strain is separated from the polypide of a Sarcophga fuscicauda imago which is naturally infected with the entomogenous fungus, cultured on a potato dextrose agar culture medium (PDA), and inoculated into the Sarcophga fuscicauda imago for rejuvenescence and acquisition of a strain; and after the strain is subjected to monomycelial separation, a purified strain, namely Verticillium lecanii KMZW-1(CGMCC No.2728), is obtained. The strain has good growth conditions at a temperature of between 28 and 29 DEG C and with a relative humidity of more than 90 percent, and has large spore yield and high spore germination rate; and spore suspension of the strain has better pathogenicity on imagoes of Sarcophga fuscicauda, lucilia sericata, houseflies, Piophila casei and drosophila.

Description

Technical field: [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a fungus Verticillium lecanii for killing fly pests and an application thereof. Background technique: [0002] Flies are important sanitation pests. They can not only spread diseases through their bodies carrying pathogenic microorganisms, but also cause serious "pollution" to the visual environment when their population density is high. As far as fly control is concerned, it is not only an important content of preventive medicine, but also an important content of building a civilized and healthy town, which is necessary for developing tourism, creating a good investment environment, and attracting foreign capital. Through the efforts of several generations of scientists, the classification, life history and ecological habits of hygienic pests have been basically clarified. At the same time, the development of insecticides has also developed from the fir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 吴国星
Owner YUNNAN AGRICULTURAL UNIVERSITY
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