61 results about "Verticillium lecanii" patented technology

The invention belongs to the technical field of biological control of pests and relates to a Lecanicillium lecanii and application of the Lecanicillium lecanii to control of greenhouse pests. The strain is Lecanicillium lecanii, is preserved in CGMCC (China General Microbiological Culture Collection Center) with the preservation number CGMCC No.8453, can be used for controlling greenhouse pests like whiteflies, aphids and thrips, and can be used in pollution-free vegetable and green food vegetable production greenhouses.

The invention relates to a Paenibacillus polymyxa Jaas ed2 with the functions of disease prevention and growth promotion to crops and application thereof, and belongs to the technical field of intensive agriculture production. The bacterial strain Jaas ed2 adopted by the invention has been preserved at the General Microbiological Culture Collection Center of China Microbiological Culture Collection Administration Committee on Sep. 18th, 2012 with the preservation number of CGMCC No.6580. The bacterial strain has the antagonistic activity to verticillium lecanii of eggplant, oxysporum of watermelon and other various pathogenic fungi, can enter plants of eggplants and watermelons in case of lower concentration of bacterium for colonization and conduction in the crops, can be used for promoting growth and preventing diseases of crops, and particularly has excellent control efficiency to soil-borne vascular bundle diseases, is low in production cost, simple to operate, facilitates ecological environment protection for farmlands, and reduces pesticide residue when used as a preparation, thereby meeting requirements for production of nuisance free agricultural products in China.

The invention relates to a Lecanicilliumpsalliotae strain used for preventing and treating diaphorina citri and belongs to the technical field of microorganisms. The Lecanicilliumpsalliotae strain ZJLP09 is registered and preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCCNo.7551. The Lecanicilliumpsalliotae strain ZJLP09 has strong pathogenicity on diaphorina citri; compared with a congeneric verticillum lecanii strain, the Lecanicilliumpsalliotae strain ZJLP09 has a stronger effect on the diaphorina citri and can be developed into a biological fungi-proofing preparation used for preventing and treating the diaphorina citri; and compared with the existing chemical agent used for preventing and treating the diaphorina citri, the Lecanicilliumpsalliotae strain ZJLP09 has the characteristics of high efficiency, low toxicity, low residue, no pollution and difficulty in formation of drug resistance and has a good market application prospect.

The invention discloses a Bacillus licheniformis 201, which is preserved in China Center for Type Culture Collection that is located in Wuhan University, Logosun, Wuchang, Wuhan, Hubei, and with CCTCC NO. of M207092. The invention simultaneously discloses a preparation method and applications of the Bacillus licheniformis 201, which has strong bacteriostasis of banana wilt fusarium, wide bacteriostasis spectrum and antagonism to various pathogenic fungi including banana wilt fusarium, watermelon wilt fusarium, tomato wilt fusarium, banana colletotrichum, gloeocystidium colletotrichum, corn leaf spot curvularia, cotton verticillium lecanii, gibberella zeae, and the like, and the Bacillus licheniformis 201 has high control efficiency, good environmental security and good development and application prospect.

The invention discloses a compound insecticide of bacillus thuringiensis, verticillium lecanii and aconite alkaloid and a preparation method thereof. The compound biotic insecticide is mainly mixed through the fermentation liquor of bacillus thuringiensis and the meristem of the verticillium lecanii or blastospore and the aconite alkaloid with proper auxiliary agent according to certain proportion, wherein the mass percentage of the main medicine and the auxiliary agent respectively accounts for 50 percent to 90 percent and 10 percent to 50 percent. The compound insecticide has the advantagesthat the compound insecticide is broad-spectrum, has control effect to various crop pests, and particularly has obvious effect to homoptera pets, lepidoptera pets and coleopteran pets; the desinsection speed is high; the lasting period is long; pets are difficult to develop drug resistance; the compound insecticide is safe to people and animals, environment and natural enemies; the manufacturing process is simple, the material sources are wide, and the cost is low; and the preparations are multiple, and the application field is wide.

The invention discloses a compound desinsection primary agent and pesticide consisting of verticillium lecanii and nimbin. The primary agent is a mixture of the verticillium lecanii and the nimbin, wherein, the mass ratio between the verticillium lecanii and the nimbin is 100:0.05-0.30; while the pesticide consists of a primary agent and a secondary agent; according to mass percent, the primary agent accounts for 20-60%, and the secondary agent 40-80%. The compound pesticide of the invention can be prepared into wettable powder, fungal spore suspending agent and medicinal oil; in addition, the compound pesticide of the invention has better effect in preventing and controlling the pests than the preventing effect when one medical preparation alone such as the nimbin is used and can effectively reduce the use of the nimbin in practical production. Through long-term infection biological study and interior biological assay, the verticillium lecanii has strong infection desinsection effect on a plurality of pests, the sucking pests in particular. The compound pesticide consisting of the verticillium lecanii and the nimbin of the invention has good effect in preventing and controlling pests such as white fly, plant louse, diamond back moth and the like.

The invention relates to a verticillium lecanii pesticide as well as the application thereof, and belongs to the plant protection and pesticide technology field. The bacterial strain provided by the invention is verticillium lecanii K1207 with CGMCC No.2326. The invention produces the bacterial strain via liquid and solid two-phase fermentation, and is characterized in that a wheat bran and bran coat culture medium is adopted as the solid culture medium. The cultured product can be added with and mixed with a wetting agent, a bulking agent and a stabilizing agent to obtain wettable powder after being parched and being crumbled. The inventive verticillium lecanii bacterial strain has high virulence, thereby having good control effects on pests such as green peach aphids, urolcueon formosanums, acyrthosiphon dirhodums, cabbage aphids, cabbage worms, diamondback moths, and greenhouse whiteflies. The materials in fermentation process are cheap and have wide sources, which is suitable for industrialized production without environmental pollution.

The invention discloses a verticillium lecanii quick-release dispersible tablet and a preparation method thereof. Verticillium lecanii concentrated fermentation liquor or dry spore powder thereof is used as a main effective component. Synergist, surface active agent, protective agent, disintegrating agent, wetting agent, filler and lubricant are added as well. The preparation method is implementedby the following steps: 2 / 3 disintegrating agent and other auxiliary agents, together with verticillium lecanii, are added into a mixer for uniform mixing according to a certain proportion; then thewetting agent with an appropriate amount is added for mixing so that the mixture obtained from the previous step is changed into a wet material; the wet material then undergoes 20-mesh screening so asto be prepared into wet grains; after 24-48-hour drying at the normal temperature in a relatively germfree condition, the rest 1 / 3 disintegrating agent is added into the dry grains, and then the grains are weighed; the lubricant with a weight accounting for 0.5-0.6% of the grain weight is added for full and uniform mixing, and then the grains are sent to a tablet machine to be pressed into largetablets so that the finished product can be obtained. The verticillium lecanii quick-release dispersible tablet has simple production process and wide material source, is easy for industrial production, can be quickly disintegrated in water to release effective components, takes effect quickly, does not cause dust pollution, has a wide range of prevention and cure and can kill insect and bacteriostasis simultaneously.

The invention discloses a lecanicillium lecanii oil solution, a preparing method thereof and applications of the oil solution in agricultural pest control. A lecanicillium lecanii oil suspension is prepared from following components by weight: 10-30% of lecanicillium lecanii spore powder, 5-20% of an emulsifier, 1-3% of a stabilizing agent and 47-84% of plant oil. The oil suspension is advantageous in that: the spore storage time is long, and after storage at room temperature for 15 months, the rate of live spores is more than 80%; the cost is low, and the amount of oil used for dilution of the oil solution is largely saved; the oil suspension meets conventional pesticide using custom; operation in the field is simple, wherein the oil suspension is directly diluted with water by 1-10 times and then sprayed by an ultra-low volume technology, or is diluted with water by 1000-2000 times and then sprayed in a normal amount; and in a dry environment, the product has good control effects for apple yellow aphids and alfalfa aphid.

The invention discloses a compound desinsection primary agent and a pesticide consisting of verticillium lecanii and abamectin. The primary agent is a mixture of the verticillium lecanii and the abamectin, wherein, the mass ratio between the verticillium lecanii and the abamectin is 100:0.1-0.5; through long-term infection biological study and interior biological assay, the verticillium lecanii has strong infection desinsection effect on a plurality of pests, the sucking pest in particular. The compound pesticide consisting of verticillium lecanii and abamectin of the invention has good effect in preventing and controlling pests such as trialeurodes vaporariorum westwood, bemisa babaci, plant louse, diamond back moth, thrips palmi karny and the like, and has certain effect in controlling mite pests.

The invention discloses a VOCs (volatile organic compounds) purification system for waste gas of petrochemical industry. The system comprises a waste heat exchanger, a gas overheat protection device, a hot gas mass flow meter, a hybrid gas flow regulation valve, a cloth bag type dust remover, a pulse ultraviolet activation-microorganism degradation reactor, a smoke-gas-water washing purification tower, and an induced draft fan. The system creatively utilizes the suitability and the bioactivity degradation function of a Verticillium lecanii KM9083 strain to high-concentration VOCs, VOCs elements in the waste gas are finally decomposed into H2O and CO2, and the biological degradation efficiency of the strain to the VOCs can meet purification treatment requirements of the industrial waste gas. Meanwhile, the system utilizes an ultraviolet radiation resistance function of a benzophenone-hexamethyl phosphorotriamides combination, and adds the medicament to the Verticillium lecanii KM9083 strain in advance, thereby effectively preventing a biological inhibition function of ultraviolet rays to the strain.

The invention relates to the phytopathology, and particularly relates to cotton endophytic fungi CEF-642 for controlling cotton verticillium wilt. The CEF-642 can be cultured on an artificial culture medium, no special requirements are imposed on the culture conditions, and the industrialized production and popularization and application are facilitated. The indoor bacteriostasis test indicates that the verticillium wilt control effect is good. The confront culture result shows that the inhibition ratio of CEF-642 on the cotton verticillium wilt can reach 83.8%, the inhibition ratio of the volatile metabolic product on the mycelial growth of verticillium lecanii is 81.6%, and the non-volatile metabolic product can completely inhibit the growth of verticillium lecanii. The greenhouse test result shows that the occurrence of verticillium wilt can be controlled well by soaking the cotton seeds with the culture filtrate of CEF-642, and the best control effect can reach 75%; the control effect of the CEF-642 culture matrix seedling growing method on the verticillium wilt is relatively outstanding, and the control efficiency can reach 73.1%. The talaromyces flavus CEF-642 is used as a biocontrol agent which does not influence the normal growth of cotton, avoids toxic and side effect and is safe to human and livestock and environment-friendly.

The invention discloses an insecticide composition for preventing and controlling injurious insects such as mealybugs. Active ingredients of the insecticide composition comprise spirotetramat and verticillium lecanii, wherein the mass percent of the spirotetramat and the verticillium lecanii in the insecticide composition is 1-45%; and the mass ratio of the spirotetramat to the verticillium lecanii is (0.1-40): 1. The insecticide composition disclosed by the invention is mainly used for the prevention and control of injurious insects such as mealybugs, has the advantages of being synergistic, good in permeability, high in activity, stable in effect, long in persistent effect, small in environmental influences, strong in stickiness and evaporation-resistant, can be used for ultralow-volume plane sprays to control a plurality of crop insects at the same time, and has good application and popularization values.

The invention discloses a compound desinsection primary agent and pesticide consisting of verticillium lecanii and imidacloprid. The primary agent is a mixture of the verticillium lecanii and the imidacloprid, wherein, the mass ratio between the verticillium lecanii and the imidacloprid is 100: 5-50; while the pesticide consists of a primary agent and a secondary agent, according to mass percent, the primary main agent accounts for 5-50%, and the secondary agent 50-95%. Through long-term infection biological study and interior biological assay, the verticillium lecanii has strong infection desinsection effect on a plurality of pests, the sucking pest in particular. The compound pesticide consisting of verticillium lecanii and imidacloprid of the invention has good effect in preventing and controlling pests such as white fly, plant louse, diamond back moth and the like.

The invention discloses a microbial pesticide for preventing and treating fungal diseases. The microbial pesticide comprises the following materials in parts by weight: 22-40 parts of verticillium lecanii, 5-17 parts of corn flour, 11-20 parts of polyisobutylene succinimide, 25-36 parts of malic acid, 15-32 parts of diatomite, 3-7 parts of metarhizium powder, 15-30 parts of trichoderma harzianum, 5-19 parts of amino-oligosaccharin, 6-9 parts of beta-cypermethrin, 45-62 parts of streptomyces diastatochromogenes D and 15-18 parts of arteannuin. The microbial pesticide is applied to preventing and controlling fungal diseases of plants and is similar with or slightly superior to general chemical agents in effect, and a new way is provided for the development of agricultural fungicides.

The present invention discloses an insect disinfestation main agent compounded by verticillum lecanii and acetamiprid and an insecticide thereof. The main agent is the mixture of verticillum lecanii and acetamiprid. The mass ratio between verticillum lecanii and acetamiprid is 100: 1.5-12. The insecticide is composed of the main agent and assistant agent. According to the weight percentage, the main agent is 22-61%, and the assistant agent is 39%-78%. After long-time infection biology research and indoor biological assay, the verticillum lecanii has strong infection insect killing effect to a plurality of insects, especially the piercing-sucking mouthparts insects. The insecticide compounded by verticillum lecanii and acetamiprid has excellent control efficiency to insects of whitefly, aphids, diamondback moth, etc.

The invention relates to an entomogenous fungus-Verticillium lecanii, which is particularly effective in preventing and treating familiar dipterous fly insects. A wild strain is separated from the polypide of a Sarcophga fuscicauda imago which is naturally infected with the entomogenous fungus, cultured on a potato dextrose agar culture medium (PDA), and inoculated into the Sarcophga fuscicauda imago for rejuvenescence and acquisition of a strain; and after the strain is subjected to monomycelial separation, a purified strain, namely Verticillium lecanii KMZW-1(CGMCC No.2728), is obtained. The strain has good growth conditions at a temperature of between 28 and 29 DEG C and with a relative humidity of more than 90 percent, and has large spore yield and high spore germination rate; and spore suspension of the strain has better pathogenicity on imagoes of Sarcophga fuscicauda, lucilia sericata, houseflies, Piophila casei and drosophila.

The invention discloses a predatory mite and verticillum lecanii combined-application biopesticide and a preparing and application method thereof and belongs to the field of plant insect pest prevention. The predatory mite and verticillum lecanii combined-application biopesticide is prepared by wheat bran, predatory mites and verticillum lecanii conidial powder in a mixed mode. According to the ratio, each kilogram of wheat bran is mixed with 1.5 * 105-2.0 * 105 predatory mites and 3-5 grams of verticillum lecanii conidial powder. The preparing method comprises the steps that 1. materials are prepared, wherein the verticillum lecanii conidial powder and the predatory mites are produced; and 2. the predatory mites and the verticillum lecanii conidial powder are mixed. The application method comprises the steps that on March to April or September to October each year, the combined biopesticide is applied in a broadcasting or powder spraying mode, so that the pesticide is evenly distributed on leaves, according to the using amount, 8-12 grams are sprayed to fruit trees at least 5 years old, 4-6 grams are sprayed to fruit trees at most 5 years old, and 450-550 grams are sprayed to each acre of vegetable and flowers.

It is intended to provide a microbial pesticide for controlling plant disease damage by applying a microbial pesticide which contains a fungus Verticillium lecanii capable of inhibiting the growth of plant pathogenic microorganisms to plants such as fruits and vegetables; and a soil conditioner for exerting a soil conditioning effect by applying a microbial pesticide for controlling soil disease damage which contains the fungus Verticillium lecanii to soil. By applying, as a microbial pesticide, the fungus Verticillium lecanii, preferably a strain capable of fixing to plant surface and / or a strain capable of fixing to plant root surface and / or capable of fixing to rooting zone to plants, the growth of not only pest insects but also plant pathogenic microorganisms can be remarkably inhibited. By applying it to soil, moreover, the growth of pathogenic microorganisms occurring in plant roots and the rooting zone thereof can be inhibited. Thus, the soil can be conditioned without a risk of the occurrence of pathogenic microorganisms even in the soil after transplanting.

The invention discloses a preparation method of a yield-increasing disease-resistant biological bacterial fertilizer for field crops. The preparation method comprises the following steps: step 1, preparing a first complex microbial inoculant and a second complex microbial inoculant; step 2, screening a plurality of strains from a strain library of verticillium lecanii, beauveria bassiana and metarhizium anisopliae, selecting three fungal strains which have the smallest antagonism with the second complex microbial inoculant obtained in the step 1 from the strains, and carrying out enlarged culture; step 3, preparing a coating agent; step 4, fermenting; step 5, granulating and coating; and step 6, carrying out low-temperature drying in vacuum. The problems that in the prior art, a field biological bacterial fertilizer prepared from plant ash, furfural residues and mushroom dregs does not contain various active microorganisms with different functions, the colonization survival performance of the microorganisms is low, and the disease prevention and insect prevention effects are not achieved can be solved.

A verticillium plasminogen is a fermentation liquid to be salted out and deposited to be desalinated by dialysis then to be chromatographed by DEAE and active peaks are collected to get rough enzymes after frozen and dried to be prepared with electrophoresis to be washed and dewateres to and get pare verticillium plasminogen with the molecular weight of 33KD, isoelectric point of 8.5 which has the fibrinolysis activation directly degradating fibrin and fine thrombus solving action.

The invention relates to a pair of specific primers for amplifying Verticillium lecanii. The invention solves the problem of lack of primers for amplifying the Verticillium lecanii target sequence from a sample DNA. The specific primers for amplifying Verticillium lecanii are (5'-CGGCGTCCGGACGCGGACCCAG-3' and VR(5'-CCCCAACGCCGACTTCCCCGAG-3'). The primers provided by the invention can accurately amplify the target segment of Verticillium lecanii.

The invention discloses verticillium lecanii LL-01 and a biocontrol application thereof, and belongs to the technical field of microorganisms. The preservation number of the strain is CGMCC No.21940. Under conditions of 26 DEG C optimal to growth and spore production, photoperiod of 6L: 18D, fructose as a carbon source and casein as a nitrogen source, the verticillium lecanii LL-01 has high growth speed, high spore yield, and accumulative fatality rates on planococcus lilacinus and phenacoccus solani ferris at different instars up to 84.09%-97.62% and 89.89%-98.85% respectively. The verticillium lecanii LL-01 has good biocontrol potential, can be used for replacing part of chemical pesticides for preventing and controlling mealybugs so as to reduce usage of chemical pesticides, and facilitates to maintain ecological balance.

The invention discloses a method for keeping the toxicity of verticillium lecanii to host insects. The method comprises the steps of adding filtrate of ground host insects into a verticillium lecanii pda culture medium, and inoculating verticillium lecanii spores after sterilizing; then, culturing in an illumination incubator at a proper temperature; after mycelia grow to generate spores, scraping the spores by using a scraper in an aseptic operating platform; and carrying out liquid fermentation culture or preparing a suspension with a certain concentration to be directly used for preventing and controlling pests. Compared with the prior art, the method has the advantages that the toxicity of the verticillium lecanii to the host insects can be kept for a long term, and the verticillium lecanii can be domesticated according to different host insects to prepare a special insecticide for certain pests, so that more effective preventing and controlling effects can be achieved. In addition, strains domesticated according to different host insects can be mixed to apply according to the kinds of the prevented and controlled pests, so that the effect is better.

The invention relates to a GhPAO protein. The GhPAO protein has an amino acid sequence shown as SEQ ID No. 2 or an amino acid sequence which is obtained by replacing, deleting or adding one or more amino acids and has equivalent functions. A cloned GhPAO gene helps to reveal a molecular mechanism of interaction between cotton and Verticillium lecanii, provides an important theoretical basis for studying a metabolic pathway for a plant to resist Verticillium wilt, and provides a more valuable candidate functional gene for improving genetic traits in the field of plant genetic engineering; the verticillium wilt resistance of a transgenic plant obtained from the GhPAO gene is significantly improved, which lays the foundation for further transforming an important agricultural crop so as to improve the disease resistance of the crop; therefore, the GhPAO gene has relatively good application prospect and application value in agricultural production.

The invention discloses a method using ants to spread verticillium lecanii for control of tree trunk stomaphissinisalicis; first, a lard and brown sugar mixture with the sugar content about 5-30% is prepared, after the lard and brown sugar mixture is condensed into a bulk, the lard and brown sugar mixture is cut into 0.5-2cm<3> small pieces, the small pieces are rolled in verticillium lecanii spore powder, so that the surface of the small pieces is fully covered with verticillium lecanii spore, and a verticillium lecanii spore release block is prepared; the verticillium lecanii spore release block is put in an inlet of an ant nest and an inlet of an ant path of a tree trunk for freely feeding to the ants, in the free feeding process, the verticillium lecanii spore may stick to epidermis, mouthparts and limbs, when the verticillium lecanii spore contacts stomaphissinisalicis hiding in the tree trunk, the verticillium lecanii spore may be transferred to the stomaphissinisalicis, and after the stomaphissinisalicis body surface is infected by the verticillium lecanii spore, the stomaphissinisalicis may die due to infection. The method has the advantages of good control effect, low cost and environmental protection.

The invention discloses a selective culture medium for quantitatively detecting verticillum lecanii and an application thereof. The selective culture medium for quantitatively detecting verticillum lecanii is prepared from the following components by weight: 10-20g of oatmeal, 15-20g of agar powder, 0.1-1.0g of bacterial bacteriostatic agent, 0.1-1.0g of fungus bacteriostatic agent and 1000ml of distilled water. The selective culture medium for quantitatively detecting verticillum lecanii has the advantages that interference of bacteria to target fungus detection is fully expelled by means of an inhibiting and killing effect of antibiotics to bacteria; influence of other funguses in a to-be-detected sample is eliminated by means of characteristics of sensitivity difference of different funguses on the fungus bacteriostatic agent as well as the difference of different funguses in utilization capacity of a carbon source, thereby achieving the purpose of effectively and quantitatively detecting verticillum lecanii. The culture medium is easily available in component, low in price, simple to prepare and convenient to use.

The invention discloses an insecticide jointly using matrine and verticillium lecanii. The insecticide uses matrine and verticillium lecanii as main active components. The insecticide has the advantages that the synergistic action of composition of matrine and verticillium lecanii is obvious, the usage amounts of matrine and verticillium lecanii are reduced, the use cost is reduced, the environment-friendly effect is realized, the drug resistance is not produced, and the strong application potential is realized in the biological prevention and control of bemisia tabaci.

The invention relates to an agricultural insecticidal composition containing Verticillium lecanii. The active ingredients of the insecticidal composition comprise a synergistically effective amount of Verticillium lecanii, and one of indoxacarb and flubendiamide, wherein a weight ratio of the Verticillium lecanii to the indoxacarb is (1-89):(89-1); and a weight ratio of the Verticillium lecanii to the flubendiamide is (1-49):(89-1). The composition can be processed to form a wettable powder, a suspending agent, a water-dispersible granule or a microcapsule, and the active ingredients also can be spatially arranged in a separate manner to reduce pest induced plant damages and post-harvest loss. Compared with single ingredients, the composition has the advantages of obvious synergistic and persistent effects, reduction of the dosage of pesticides, reduction of the residual amount of pesticides on crops, and reduction of environmental pollution.

The invention discloses a compound desinsection primary agent and pesticide consisting of verticillium lecanii and imidacloprid. The primary agent is a mixture of the verticillium lecanii and the imidacloprid, wherein, the mass ratio between the verticillium lecanii and the imidacloprid is 100: 5-50; while the pesticide consists of a primary agent and a secondary agent, according to mass percent,the primary main agent accounts for 5-50%, and the secondary agent 50-95%. Through long-term infection biological study and interior biological assay, the verticillium lecanii has strong infection desinsection effect on a plurality of pests, the sucking pest in particular. The compound pesticide consisting of verticillium lecanii and imidacloprid of the invention has good effect in preventing andcontrolling pests such as white fly, plant louse, diamond back moth and the like.