Microbial pesticide inhibiting the outbreak of plant disease damage
A technology of microbial pesticides and plant pathogenic bacteria, applied in plant growth regulators, botany equipment and methods, preservation of human or animal bodies, etc., can solve problems such as toxicity, harmful organisms, and impacts of humans and animals
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Embodiment 1
[0064] [Biological Control of Strawberry Botrytis]
[0065] (Test microorganisms)
[0066] What the inventor tested in the following experiment was the isolated Verticillium lecanii A-2 strain, the Verticillium lecanii B-2 strain, the commercially available Verticillium lecanii microbial pesticide Vertalec (バ-タレツク) and Four strains of Verticillium lecanii from Mycotal (マイコタ-ル).
[0067] (Antagonism of Verticillium lecanii cultured in vitro)
[0068] To study the antagonism of Verticillium lecanii cultured in vitro against Botrytis cinerea in strawberry. The experiment was carried out by dual culture of 4 strains of Verticillium lecanii and Botrytis cinerea. Strawberry Botrytis cinerea Aga-Disk was placed on one side of a plate of Potato Dextrose Medium (PDA: manufactured by Sigma Co., Ltd.), and Aga-Disk of Verticillium lecanii cultivated on Potato Sucrose Medium (PSA) was placed on the same plate. the other side of the The zone of inhibition (mm) was measured after incub...
Embodiment 2
[0077] [Biological control of Fusarium wilt of cucumber]
[0078] (Test microorganisms)
[0079] 20 isolates of Verticillium lecanii listed in Table 1, including commercially available ba-tarek and miikota-lu, were tested. The fungus was cultured on a potato sucrose chitin agar (PSCA: 1 liter, 200 g potato, 20 g sucrose, 10 g chitin, 20 g agar) plate in the dark at 25±1° C. These strains were propagated for 2 weeks in horticultural soil and wheat bran (4:1, w / w). Another method is to isolate cucumber Fusarium wilt (FOM) from cucumber fruit trees in Tokachi Agricultural Experimental Field. The pathogenic bacteria were propagated on potato dextrose agar (PDA) maintained at 5±1°C.
[0080] V. lecanii
Inhibition
A-1
A-2
A-3
B-1
B-2
C-1
C-2
ATCC22611
ATCC22612
ATCC58907
ATCC58908
ATCC58909
MAFF285690
MAFF235698
MAFF235694
MAFF285696
MAFF285699
MAFF235701
Mycotal
Ver...
Embodiment 3
[0093] [Biological control of tomato Verticillium wilt]
[0094] (Test microorganisms)
[0095] Same as Example 2, 20 isolates of Verticillium lecanii containing commercially available Ba-tarek and Miikota-lu listed in Table 2 were tested.
[0096] Isolates
Inhibition
A-1
A-2
A-3
B-1
B-2
C-1
C-2
Mycotal
Vertalec
ATCC22611
ATCC22612
ATCC58807
ATCC58908
ATCC58909
MAFF235690
MAFF235693
MAFF235694
MAFF235696
MAFF285699
MAFF235701
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[0097] (Antagonism of Verticillium lecanii cultured in vitro)
[0098] To study the antagonism of Verticillium lecanii cultured in vitro against Verticillium dahliae in tomato. The experiment was carried out by dual culture of 20 strains of Verticillium lecanii and Verticillium dahliae. Verticillium dahl...
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