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Microbial pesticide inhibiting the outbreak of plant disease damage

A technology of microbial pesticides and plant pathogenic bacteria, applied in plant growth regulators, botany equipment and methods, preservation of human or animal bodies, etc., can solve problems such as toxicity, harmful organisms, and impacts of humans and animals

Inactive Publication Date: 2007-04-11
JAPAN SCI & TECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, on the other side of the excellent effect of pesticides using chemical substances, there may be some that are toxic to humans and animals, remain in the natural environment and affect other ecosystems, and what is more, due to long-term use, resistant pests and diseases, Even killing the natural enemies that exist in nature will cause problems such as the environment where harmful organisms occur

Method used

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  • Microbial pesticide inhibiting the outbreak of plant disease damage
  • Microbial pesticide inhibiting the outbreak of plant disease damage
  • Microbial pesticide inhibiting the outbreak of plant disease damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] [Biological Control of Strawberry Botrytis]

[0065] (Test microorganisms)

[0066] What the inventor tested in the following experiment was the isolated Verticillium lecanii A-2 strain, the Verticillium lecanii B-2 strain, the commercially available Verticillium lecanii microbial pesticide Vertalec (バ-タレツク) and Four strains of Verticillium lecanii from Mycotal (マイコタ-ル).

[0067] (Antagonism of Verticillium lecanii cultured in vitro)

[0068] To study the antagonism of Verticillium lecanii cultured in vitro against Botrytis cinerea in strawberry. The experiment was carried out by dual culture of 4 strains of Verticillium lecanii and Botrytis cinerea. Strawberry Botrytis cinerea Aga-Disk was placed on one side of a plate of Potato Dextrose Medium (PDA: manufactured by Sigma Co., Ltd.), and Aga-Disk of Verticillium lecanii cultivated on Potato Sucrose Medium (PSA) was placed on the same plate. the other side of the The zone of inhibition (mm) was measured after incub...

Embodiment 2

[0077] [Biological control of Fusarium wilt of cucumber]

[0078] (Test microorganisms)

[0079] 20 isolates of Verticillium lecanii listed in Table 1, including commercially available ba-tarek and miikota-lu, were tested. The fungus was cultured on a potato sucrose chitin agar (PSCA: 1 liter, 200 g potato, 20 g sucrose, 10 g chitin, 20 g agar) plate in the dark at 25±1° C. These strains were propagated for 2 weeks in horticultural soil and wheat bran (4:1, w / w). Another method is to isolate cucumber Fusarium wilt (FOM) from cucumber fruit trees in Tokachi Agricultural Experimental Field. The pathogenic bacteria were propagated on potato dextrose agar (PDA) maintained at 5±1°C.

[0080] V. lecanii

Inhibition

A-1

A-2

A-3

B-1

B-2

C-1

C-2

ATCC22611

ATCC22612

ATCC58907

ATCC58908

ATCC58909

MAFF285690

MAFF235698

MAFF235694

MAFF285696

MAFF285699

MAFF235701

Mycotal

Ver...

Embodiment 3

[0093] [Biological control of tomato Verticillium wilt]

[0094] (Test microorganisms)

[0095] Same as Example 2, 20 isolates of Verticillium lecanii containing commercially available Ba-tarek and Miikota-lu listed in Table 2 were tested.

[0096] Isolates

Inhibition

A-1

A-2

A-3

B-1

B-2

C-1

C-2

Mycotal

Vertalec

ATCC22611

ATCC22612

ATCC58807

ATCC58908

ATCC58909

MAFF235690

MAFF235693

MAFF235694

MAFF235696

MAFF285699

MAFF235701

+

+

+

++

++

+

+

+

+++

-

+

++

-

+

+++

+

+

-

-

-

[0097] (Antagonism of Verticillium lecanii cultured in vitro)

[0098] To study the antagonism of Verticillium lecanii cultured in vitro against Verticillium dahliae in tomato. The experiment was carried out by dual culture of 20 strains of Verticillium lecanii and Verticillium dahliae. Verticillium dahl...

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PUM

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Abstract

It is intended to provide a microbial pesticide for controlling plant disease damage by applying a microbial pesticide which contains a fungus Verticillium lecanii capable of inhibiting the growth of plant pathogenic microorganisms to plants such as fruits and vegetables; and a soil conditioner for exerting a soil conditioning effect by applying a microbial pesticide for controlling soil disease damage which contains the fungus Verticillium lecanii to soil. By applying, as a microbial pesticide, the fungus Verticillium lecanii, preferably a strain capable of fixing to plant surface and / or a strain capable of fixing to plant root surface and / or capable of fixing to rooting zone to plants, the growth of not only pest insects but also plant pathogenic microorganisms can be remarkably inhibited. By applying it to soil, moreover, the growth of pathogenic microorganisms occurring in plant roots and the rooting zone thereof can be inhibited. Thus, the soil can be conditioned without a risk of the occurrence of pathogenic microorganisms even in the soil after transplanting.

Description

technical field [0001] The present invention relates to the microbial pesticide that suppresses the occurrence of plant disease, specifically to the microbial pesticide containing insect parasitic filamentous fungus Verticillium lecanii, and by spreading the microbial pesticide on plants such as fruits and vegetables or applying it to plant cultivation Soil microbial pesticides that inhibit the growth of plant pathogenic bacteria and improve the soil. Background technique [0002] Due to the advances in the research on the pest control of vegetables, fruits, rice, wheat, fruit trees and other plants or the use of chemical substances for the control of soil pests attached to plant roots, at present, relying on chemical insecticides and fungicides is the mainstream. However, on the other side of the excellent effect of pesticides using chemical substances, there may be some that are toxic to humans and animals, remain in the natural environment and affect other ecosystems, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N63/30
CPCA01N63/04A01N63/30A01N25/00A01N2300/00A01N63/20
Inventor 小池正德吉田早苗阿部伦子浅野健治
Owner JAPAN SCI & TECH CORP
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