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Lecanicillium lecanii and application of Lecanicillium lecanii to control of greenhouse pests

A technology of Verticillium leek, greenhouse, applied in application, pesticides, fungi and other directions, can solve problems such as excessive pesticide residues in agricultural products and farmland environmental pollution.

Inactive Publication Date: 2014-12-10
谢明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main means of controlling pests is chemical control, such as spraying chemical pesticides such as organophosphorus, pyrethroids, and imidacloprid. The extensive use of these pesticides has caused serious pesticide residues in agricultural products and farmland environmental pollution. It is urgent to develop biological control to replace or reduce use of chemical pesticides

Method used

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  • Lecanicillium lecanii and application of Lecanicillium lecanii to control of greenhouse pests
  • Lecanicillium lecanii and application of Lecanicillium lecanii to control of greenhouse pests
  • Lecanicillium lecanii and application of Lecanicillium lecanii to control of greenhouse pests

Examples

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Effect test

Embodiment 1

[0039] Embodiment 1: the mass culture technique of bacterial strain spore

[0040] Cultivate the strain of the modified strain in a shaker flask at 23°C for 65 hours, use it as a seed, connect it to rice grains after high-temperature sterilization with a water content of 75% in a volume of 8%, and put it in a sterile bag at 23°C Cultivate for 6 days, and open the bag for 2 days. After finishing the cultivation, dry in a drying room at 35° C. for 4 days, then crush the dried culture and sieve (250 mesh, 320 mesh, 400 mesh) to obtain pure sporopollen (containing 95% spores). This pure sporopollen can be used to formulate formulations for field application to control pests in greenhouses.

Embodiment 2

[0041] Embodiment 2: Control greenhouse vegetable whitefly and aphids

[0042]The resulting pure sporopollen will be produced according to: Verticillium lecanii pure sporopollen (95% spore-containing) 10%, glucose (powder) 2%, sodium lignosulfonate 1%, starch 3%, dispersant BD553 %, wetting agent BENOL790A3%, diatomaceous earth 78%, mixed evenly. The preparation can be applied in the field. The preparation is diluted 1500 times with water, sprayed to prevent whitefly in greenhouses, and when the indoor temperature is 23-27° C. and the relative humidity is above 85%, the parasitic rate to pests reaches 90% within 6 days.

Embodiment 3

[0043] Example 3: Control of Bemisia tabaci and thrips on cucumbers in the greenhouse

[0044] The resulting pure sporopollen will be produced according to: Verticillium lecanii pure sporopollen (containing spore 95%) 10%, glucose (powder) 2%, starch 2%, dispersant 84254%, wetting agent MORTWET4%, silicon Alginate 78%, mixed evenly. The preparation can be applied in the field. The preparation is diluted 1500 times with water, sprayed to control whitefly and thrips, and when the temperature in the greenhouse is 26° C. and the relative humidity is 85%, the parasitic rate to pests reaches 90% within 6 days.

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Abstract

The invention belongs to the technical field of biological control of pests and relates to a Lecanicillium lecanii and application of the Lecanicillium lecanii to control of greenhouse pests. The strain is Lecanicillium lecanii, is preserved in CGMCC (China General Microbiological Culture Collection Center) with the preservation number CGMCC No.8453, can be used for controlling greenhouse pests like whiteflies, aphids and thrips, and can be used in pollution-free vegetable and green food vegetable production greenhouses.

Description

technical field [0001] The invention belongs to the technical field of pest biological control, and relates to a fungal strain Lecanicillium lecanii CGMCC No.8453 (original number GLLE0906) derived from insects and its application in greenhouse pest control, which can be used to control greenhouse pest whitefly , aphids, thrips, and can be used in greenhouses for the production of pollution-free vegetables and green food vegetables. Background technique [0002] In recent years, people's living standards have been continuously improved, and the requirements for food quality are getting higher and higher, and the production of pollution-free food and green food has emerged as the times require. At present, there are 50 million mu of greenhouse vegetables in our country, which are the main source of vegetables in winter and spring in northern my country, and have important national economic significance. The main pests that occur in winter and spring vegetable greenhouses are...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 谢明张艳军张召荣尉文彬
Owner 谢明
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