Method for keeping toxicity of verticillium lecanii to host insects

Abstract

The invention discloses a method for keeping the toxicity of verticillium lecanii to host insects. The method comprises the steps of adding filtrate of ground host insects into a verticillium lecanii pda culture medium, and inoculating verticillium lecanii spores after sterilizing; then, culturing in an illumination incubator at a proper temperature; after mycelia grow to generate spores, scraping the spores by using a scraper in an aseptic operating platform; and carrying out liquid fermentation culture or preparing a suspension with a certain concentration to be directly used for preventing and controlling pests. Compared with the prior art, the method has the advantages that the toxicity of the verticillium lecanii to the host insects can be kept for a long term, and the verticillium lecanii can be domesticated according to different host insects to prepare a special insecticide for certain pests, so that more effective preventing and controlling effects can be achieved. In addition, strains domesticated according to different host insects can be mixed to apply according to the kinds of the prevented and controlled pests, so that the effect is better.

Description

technical field [0001] The invention relates to a method for maintaining the toxicity of Verticillium lecanii to host insects. Background technique [0002] Verticillium lecanii (Verticillium lecanii) is an entomopathogenic fungus, belonging to the Deuteromycetes subclass Rhizoctonia class Verticillium creeping group, its host range includes Coleoptera, Lepidoptera, Hemiptera, Thysanoptera order, Orthoptera, Hymenoptera, Diptera, Collembola, gall mites, nematodes, and even parasitic plant pathogens. It has good genetic stability and is harmless to humans, livestock, poultry and plants. It is a kind of Biocontrol fungi with broad application prospects, but in the application process, after several generations of continuous inoculation and culture on artificial medium, their toxicity to pests will be significantly reduced, or even lost, which seriously restricts the further development of Verticillium lecanii resources. develop and use. Therefore, it is urgent to establish a...

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Problems solved by technology

[0002] Verticillium lecanii (Verticillium lecanii) is an entomopathogenic fungus, belonging to the Deuteromycetes subclass Rhizoctonia class Verticillium creeping group, its host range includes Coleoptera, Lepidoptera, Hemiptera, Thysanoptera order, Orthoptera, Hymenoptera, Diptera, Collembola, gall mites, nematodes, and even parasitic plant pathogens. It has good genetic stability and is harmless to humans, livestock, poultry and plants. It is a kind of Biocontrol fungi with broad application prospects, but in the application process, after several generations of continuous inoculation and culture on artificial medium, their toxicity to pests will be significantly reduced, or even lost, which seriously restricts the further development of Verticillium lecanii resources. develop and use

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