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Verticillium lecanii LL-01 and biocontrol application thereof

A biocontrol technology for Verticillium lecanii, applied in the field of microorganisms, can solve the problem of few strain resources

Active Publication Date: 2021-06-29
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are about 1,000 kinds of entomopathogenic fungi recorded in the world, but only F061 and FM-03 in Metarhizium anisopliae have been excavated for the prevention and control of P. Selected strain resources are still relatively few.

Method used

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  • Verticillium lecanii LL-01 and biocontrol application thereof
  • Verticillium lecanii LL-01 and biocontrol application thereof
  • Verticillium lecanii LL-01 and biocontrol application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Identification of entomopathogenic fungi

[0032] Morphological observation: Pick a single spore of the strain LL-01 and inoculate it in the center of the Chase medium plate, and place it in GZX-250BSH-Ⅲ at a temperature of (25±1)°C, a relative humidity of (90±5)%, and a photoperiod of 12L:12D After culturing for 10 days in a type light incubator, first use a DSLR-A450 digital SLR camera to observe and photograph the morphological characteristics of the colony, and measure the diameter of the colony; Morphological characteristics of cells and conidia, and preliminary identification of the taxonomic status of entomogenous fungi.

[0033] the result shows:

[0034] Strain LL-01 grows well on the Chapei medium plate; the colony is nearly round, initially white, and radially expands and grows outward. After 10 days, the diameter can reach 3.82-4.24cm, and the middle area is slightly pale pink, ( figure 1 -A). Mycelia are elongated, branched and separated, 1.99-2.37 μm wi...

Embodiment 2

[0041] Optimizing the culture conditions of entomopathogenic fungi:

[0042] Temperature selection: Pick a single spore of Verticillium lecanii LL-01 and inoculate it in the center of the Chapei medium plate, and then place it in an incubator at 22, 26 and 30°C for dark cultivation, and each treatment takes 3 After culturing for 10 days, measure the colony diameter with the cross method; then add 10 mL of sterile water containing 0.05% Tween-80 to each treated petri dish, scrape off the conidia with a sterilized glass slide to prepare a spore After the suspension was filtered through 3 layers of gauze, the sporulation of each treatment was observed and measured under the E200 biological microscope with a Neubauer hemocytometer.

[0043] Illumination selection: Pick a single spore of Verticillium lecanii LL-01 and inoculate it in the center of the Cha's medium plate, and then place it in three photoperiods and temperatures of 6L:18D, 12L:12D and 18L:6D (26±1 ) ° C in a light i...

Embodiment 3

[0056] Biocontrol potential assessment of entomopathogenic fungi:

[0057] Determination of pathogenicity: On the basis of optimizing the culture conditions, take Verticillium lecanii LL-01 cultured for 10 days, first add 10 mL of sterile water containing 0.05% Tween-80 to the culture dish, and then use a sterilized glass The conidia were scraped off the slices, and then filtered through three layers of gauze to obtain the spore stock solution. After mixing thoroughly, the spore concentration was calculated; the spore stock solution was diluted to 1×10 with sterile water. 8 , 1×10 7 , 1×10 6 , 1×10 5 and 1×10 4 5 treatment concentrations of spores / mL, with 0.05% Tween-80 sterile water as the control, and each treatment was repeated 3 times. Aiming at the Pneumococcus chinensis, take a plastic fresh-keeping box with a diameter of 12cm and a height of 10cm, and put 1 custard apple fruit with a growth rate of 90 days in each fresh-keeping box; dig a vent hole with a diameter ...

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Abstract

The invention discloses verticillium lecanii LL-01 and a biocontrol application thereof, and belongs to the technical field of microorganisms. The preservation number of the strain is CGMCC No.21940. Under conditions of 26 DEG C optimal to growth and spore production, photoperiod of 6L: 18D, fructose as a carbon source and casein as a nitrogen source, the verticillium lecanii LL-01 has high growth speed, high spore yield, and accumulative fatality rates on planococcus lilacinus and phenacoccus solani ferris at different instars up to 84.09%-97.62% and 89.89%-98.85% respectively. The verticillium lecanii LL-01 has good biocontrol potential, can be used for replacing part of chemical pesticides for preventing and controlling mealybugs so as to reduce usage of chemical pesticides, and facilitates to maintain ecological balance.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and more specifically relates to a strain of Verticillium lecanii LL-01 and its biocontrol application. Background technique [0002] Planococcus lilacinus and Phenacoccus solani are two kinds of worldwide flower and fruit pests, which can harm 35 families such as cocoa, custard apple, guava, sheep hoof beetle, variable leaf tree and rhododendron More than 100 kinds of fruits, forest trees and ornamental plants were listed as imported plant quarantine pests by my country in 2007; There are 101 genera and 131 species of ornamental plants and economic crops. In 2014, the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China listed them as the key pests of imported succulent plants. Although these two species of quarantine mealybugs have not been widely distributed in China, the preliminary survey of this study found that they have caused se...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCC12N1/14A01N63/30Y02A50/30
Inventor 黄鹏姚锦爱余德亿
Owner INST OF PLANT PROTECTION FAAS
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