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Compound insecticide of bacillus thuringiensis, verticillium lecanii and aconite alkaloid and preparation method thereof

A technology of Verticillium cereus and Bacillus aureus, which is applied in the directions of pesticides, biocides, animal repellents, etc., can solve the problems of unclear active ingredients, unsatisfactory control effects, limitations in development and application, etc. The effect of convenient transportation, low cost and strong anti-adversity ability

Active Publication Date: 2010-03-03
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are some experimental products, such as: Bacillus thuringiensis (Bt) and abamectin (Av), Bacillus thuringiensis, avermectin and matrine, Bacillus thuringiensis, Beauveria bassiana and azadirachtin, bitter The complex biopesticides of ginseng and nicotine have complex production processes or unclear active ingredients, high production costs, and the use of a large amount of organic solvents in the extraction process of plant-derived components reduces their safety. The dosage form is single and the control effect is low. Unsatisfactory and many disadvantages limit its development and application

Method used

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  • Compound insecticide of bacillus thuringiensis, verticillium lecanii and aconite alkaloid and preparation method thereof
  • Compound insecticide of bacillus thuringiensis, verticillium lecanii and aconite alkaloid and preparation method thereof
  • Compound insecticide of bacillus thuringiensis, verticillium lecanii and aconite alkaloid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-11

[0052] Examples 1-11: Bacillus thuringiensis, Verticillium lecanii and aconitum alkaloid compound microcapsule formulation.

[0053] The compound insecticide is in the form of microcapsules, and the fermentation products of Bacillus thuringiensis and Verticillium lecanii in the main drug are fermentation broths, and the contents of each component are shown in Table 1 and Table 2, respectively.

[0054] The preparation method of microcapsule dosage form is:

[0055] A. Take a single capsule material solution and sterilize it under high pressure at 121°C for 25 minutes, and sterilize the auxiliary agent with ultraviolet light for 40 minutes. solution, then add emulsifier, active substance protective agent and antioxidant in sequence, fully stir and mix for 2 hours to obtain a mixed solution, and use an extruder with a pore size adjusted to 0.01 micron to squeeze the above mixed solution evenly into the pre-prepared product. Measure 10 times the volume (weight to volume ratio) o...

Embodiment 12-17

[0066] Example 12-17, Bacillus thuringiensis, Verticillium lecanii and aconitum alkaloid compound liquid preparation

[0067] As the compound insecticide is a liquid preparation, the fermented products of Bacillus thuringiensis and Verticillium lecanii in the main drug are both fermented liquids, and the contents of each component are shown in Table 3.

[0068] The preparation method of the liquid preparation is: sterilize the suspending agent, UV protective agent and antioxidant at 121°C for 25 minutes by autoclaving, and sterilize other auxiliaries by ultraviolet irradiation for 40 minutes, and then ferment the fermentation broth of Bacillus thuringiensis and Verticillium lecanii Liquid and aconitum alkaloid 1:1:0.1 mixture, UV protective agent, active substance protective agent, emulsifier, suspending agent and antioxidant are added to the mixer in sequence, and stirred and mixed at a speed of 150 rpm After 60 minutes, add 1-2% antifreeze agent, continue stirring and mixing...

Embodiment 18-24

[0073] Example 18-24, Bacillus thuringiensis, Verticillium lecanii and aconitum alkaloid compound powder

[0074] The compound insecticide is a compound powder. The fermentation product of Bacillus thuringiensis in the main drug is a solid as the centrifuged sediment of its fermentation liquid, and the fermentation product of Verticillium lecanii is a conidia powder cultivated in a solid; the contents of each component are shown in Table 4 .

[0075] The preparation method of Verticillium lecanii conidia powder is as follows: In a 500ml culture bottle, 200ml of Verticillium lecanii spores covered with potato culture medium are eluted with 1-2g of sterile kaolin, and peeled off together with the bacterial lawn , dried at room temperature under sterile conditions, pulverized, and passed through a 100-mesh sieve;

[0076] Pregelatinized starch was purchased from Qufu Tianli Pharmaceutical Excipients Co., Ltd.

[0077] The preparation method of the composite powder is as follows...

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Abstract

The invention discloses a compound insecticide of bacillus thuringiensis, verticillium lecanii and aconite alkaloid and a preparation method thereof. The compound biotic insecticide is mainly mixed through the fermentation liquor of bacillus thuringiensis and the meristem of the verticillium lecanii or blastospore and the aconite alkaloid with proper auxiliary agent according to certain proportion, wherein the mass percentage of the main medicine and the auxiliary agent respectively accounts for 50 percent to 90 percent and 10 percent to 50 percent. The compound insecticide has the advantagesthat the compound insecticide is broad-spectrum, has control effect to various crop pests, and particularly has obvious effect to homoptera pets, lepidoptera pets and coleopteran pets; the desinsection speed is high; the lasting period is long; pets are difficult to develop drug resistance; the compound insecticide is safe to people and animals, environment and natural enemies; the manufacturing process is simple, the material sources are wide, and the cost is low; and the preparations are multiple, and the application field is wide.

Description

technical field [0001] The invention belongs to the field of biological pesticides, in particular to a compound insecticide of Bacillus thuringiensis, Verticillium lecanii and aconitum alkaloids. Background technique [0002] In order to give full play to the respective advantages of biological pesticide components, overcome their disadvantages, reduce the cost of agricultural production, improve drug efficacy, extend the duration of efficacy, broaden the insecticidal spectrum, and develop non-toxic and environmentally friendly biological pesticides that are safe for humans, animals, the environment and natural enemies. Usually, some compatible biopesticide ingredients are reasonably combined and matched to make various compound biological preparations, so that various active substances can complement each other. [0003] There are some experimental products, such as: Bacillus thuringiensis (Bt) and abamectin (Av), Bacillus thuringiensis, avermectin and matrine, Bacillus thu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N63/02A01N43/90A01N65/32A01N25/28A01N25/12A01P7/04
CPCY02A50/30
Inventor 刘锦霞沈思远李娜杜文静李晶武建荣韩融冰张建军杨兰廷
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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