Method of producing protein feed by liquid-solid two-step fermentation method

Abstract

The invention discloses a method of producing a protein feed by a liquid-solid two-step fermentation method. The method comprises the following steps: performing liquid culture on corynebacterium glutamicum, candida utilis, bacillus subtilis, lactobacillus plantarum and aspergillus oryzae so as to obtain corynebacterium glutamicum fermentation liquor, candida utilis fermentation liquor, bacillus subtilis fermentation liquor, lactobacillus plantarum fermentation liquor and aspergillus oryzae fermentation liquor; mixing the corynebacterium glutamicum fermentation liquor, the candida utilis fermentation liquor, the bacillus subtilis fermentation liquor, the lactobacillus plantarum fermentation liquor and the aspergillus oryzae fermentation liquor so as to obtain mixed bacterium liquor; loading raw materials of soybean meal, cluster bean meal, cottonseed meal and the like into an explosion vat for performing steam explosion; after steam explosion, mixing materials, the mixed bacterium liquor and molasses in proportion, and adding an enzyme preparation so as to obtain mixed materials; conveying the mixed materials into a special modularized solid-state biological raw material continuous fermentation device for enzymolysis and fermentation, and drying the fermented materials so as to obtain the protein feed. According to the method disclosed by the invention, the modularized automatic fermentation device is adopted, the materials after explosion, the fermented bacterium liquor and the enzyme preparation are mixed, then the mixed materials are loaded into the automatic fermentation device for enzymolysis and biological fermentation, so that requirements for automatic temperature control, automatic dampness control and automatic ventilation control are met, the bioconversion rate is high, the fermentation period is short, the consumption is low, the cost is low, and mechanized and large-scale production requirements can be met.

Description

Technical field [0001] The invention involves the production of protein feed, especially the method of producing protein feed in a liquid-solid two-step fermentation method. Background technique [0002] At present, the research of biomass resources such as agricultural and sideline products and food industries and waste into feed protein is relatively popular, but because many raw materials contain a large amount of anti -nutritional factors, it is not suitable for direct use: vegetable meal category contains a lotFasterase inhibitors, antigen protein, uric acid, plant condensin, non -starch polysaccharides and other anti -nutritional factors have affected the digestion and absorption of animals in these feeds; bean residue, sweet potato residue, cassia residue, straw and other raw materials contain a large amount of fiberAnti -nutritional factors such as vegetarian and lignin require biological transformation to be absorbed and utilized by animals.However, most of the transform...

AI Technical Summary

Problems solved by technology

[0002] At present, the research on converting biomass resources such as agricultural by-products and food industry leftovers and wastes into feed protein is popular, but because many raw materials contain a large amount of anti-nutrient Factors, not suitable for direct use: plant meal contains a large number of anti-nutritional factors such as trypsin inhibitors, antigenic proteins, uric acid, plant lectins, non-starch polysaccharides, etc., which affect the digestion and absorption of these feeds by animals; soybean dregs , sweet potato dregs, cassava dregs, straw and other raw materials contain a lot of anti-nutritional factors such as cellulose and lignin, all of which need to be biotransformed before they can be well absorbed and utilized by animals

However, at present, most of the conversion of biomass resources such as agricultural by-products and food industry waste is a single solid-state fermentation method, and there are many shortcomings in the single solid-state fermentation method, especially in industrial production, such as the slow growth of bacteria , long fermentation time, easy to contaminate miscellaneous bacteria, low decomposition efficiency of anti-nutritional factors, etc.

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