The invention discloses a composition for improving skin stress response and promoting probiotics balance and a forming method. The composition is prepared from an artemisia annua leaf extract, a lactobacillus and eriodictyon californicum fermentation product extract, alpha-glucan oligosaccharide, carnosine and 1,2-pentanediol, wherein the concentration of the artemisia annua leaf extract is between 5% and 80%; the concentration of the lactobacillus and eriodictyon californicum fermentation product extract is 5% to 80%; the concentration of the alpha-glucan oligosaccharide is between 5% and 80%; the concentration of the carnosine is between 5% and 80%; the concentration of the 1,2-pentanediol is between 5% and 80%. Not only can the problem of skin fragility caused by deficiency of stratumcorneum and injuries be alleviated, but also the damage to the skin barrier caused by external stimuli can be solved; by combining various components, the skin can immediately improve cell stress response per self after being damaged to resist external irritation and damage, so that use feeling is good, the effect is fast, the preparation method is simple, and the composition is suitable for large-scale production and gives no burden to the skin.