45 results about "Tachypleus" patented technology

The invention discloses a method for detecting bacterial endotoxin in a citric acid raw material. The method comprises the following steps of firstly carrying out interference test to determine the sensitivity of tachypleus amebocyte lysate selected to detect the bacterial endotoxin in the citric acid raw material; and then diluting the to-be-detected citric acid raw material to prepare a to-be-detected solution, adding the tachypleus amebocyte lysate determined in the interference test and detecting the bacterial endotoxin. By virtue of the method for detecting bacterial endotoxin in the citric acid raw material, which is disclosed by the invention, the problem of detection of the bacterial endotoxin in the citric acid raw material for a newly developed injection product is solved; the quality of a sterile product is controlled at the source and the method is of great significance for ensuring the safety of the injection product. Since the method has a theoretical basis of biochemistry, the method is more scientific; molecular and biochemical reactions of an enzyme are applied and the method has the advantages of high specificity, strong reproducibility, high accuracy and sensitivity, no need of special instrument and equipment and strong practicability and is simple and quick to operate.

The invention discloses a method for detecting endotoxin content. The method comprises the following steps: S1. preparing at least five endotoxin standard solutions containing different concentrations; S2. respectively mixing a tachypleus amebocyte lysate diluent with each endotoxin standard solution at the ratio; S3. respectively detecting the time required when the elasticity of each mixed liquid reaches 0.1 amplitude by virtue of a thrombelastogram instrument, and drawing a bilogarithmic graph of time-endotoxin concentration to obtain a standard curve graph; and S4. mixing the tachypleus amebocyte lysate diluent with a to-be-detected sample liquid according to a specific ratio, detecting the time required when the elasticity reaches 0.1 amplitude by virtue of the thrombelastogram instrument, and comparing with the standard curve graph, so as to obtain the endotoxin content in the to-be-detected sample liquid. Compared with traditional nephelometry and electrochemical biosensor, relatively comprehensive detection can be carried out on the overall process; research on the special physiological process is facilitated; the detection time is greatly shortened; and the detection sensitivity and accuracy are synchronously improved.

The invention relates to an improved body fluid color-developing fungi 1,3-beta-D-glucan detection kit, which comprises a reaction main-agent, a main agent combination solution, a sample treatment fluid, sterile water, a standard substance and a quality control material. With Tachypleus tridentatus Leach or Limulus polyphemus Linnaeus hemocyte lysate as a main raw material, the reaction main-agent contains G factor, B factor, C factor, clottable protein and a polypeptide chromogenic substrate. By a new preparation process, the reaction main-agent has characteristics of high stability and small intra-assay and inter-assay difference. The main agent combination solution adopts a new formula. After the main agent combination solution is mixed with the reaction main-agent, Tachypleus Amebocyte Lysate endotoxin reaction branch can be effectively shielded. According to the kit, rate-method enzyme kinetics detection is carried out by ELIASA. The detection speed is fast, and anti-interference performance is strong. In addition, the preparation technology is simple, and the product has stronger stability and higher specificity and sensitivity.

The invention discloses a method for amplifying antibody marking signal or nucleic acid probe marking signal, and the main scheme is that: lipopolysaccharide of gram negative bacteria and an antibody are crosslinked, or fungi (1,3)-beta-D-glucan of fungi cell wall and the antibody or a nucleic acid probe are crosslinked, and then tachypleus amebocyte lysate and the gram negative bacteria lipopolysaccharide or fungi cell wall (1,3)-beta-D-glucan which is crosslinked with the antibody are specifically reacted, thereby improving the sensitivity of the antibody or nucleic acid probe detection. The system for amplifying antibody marking signal or nucleic acid probe marking signal can detect object substrates lower than pg level content.

The invention discloses a method for detecting endotoxin. The method includes a material with oxidation reduction activity is added in tachypleus amebocyte lysate, a sample to be tested is added in the tachypleus amebocyte lysate, the sample to be tested may comprise the endotoxin, an oxidation reduction potential corresponding to the material with the oxidation reduction activity is exerted on the tachypleus amebocyte lysate, size of current passing through the tachypleus amebocyte lysate is monitored in real time simultaneously, and concentration of the endotoxin in the sample to be tested is obtained. Logarithm linear relationship exists between the concentration of the endotoxin added in the tachypleus amebocyte lysate and a time dropping point of the current passing through the tachypleus amebocyte lysate. Viscosity change in a tachypleus amebocyte lysate solidification process is shown through an electrochemical process directly, interference of sample colors is avoided, detection of the endotoxin can be achieved rapidly and accurately, and the method for detecting the endotoxin can be widely applied to the field of drug quality control, medical equipment pyrogen monitoring and the like.

The invention relates to a method using an antibacterial composition extracted from tachypleus amebocyte lysate waste to prepare cosmetics. The method includes the steps of firstly, extracting the antibacterial composition from the tachypleus amebocyte lysate waste, to be more specific, collecting discarded emulsion sediments and discarded plasma in tachypleus amebocyte lysate production, using the discarded emulsion sediments to prepare crude tachyplesin peptide extract, using the discarded plasma to prepare crude hemocyanin extract, using the discarded plasma to prepare crude SOD extract, and mixing the three crude extract to prepare the antibacterial composition; secondly using, by mass percentage, 30-50% of antibacterial composition, 2-4% of olive oil, 1-2% of vitamin E acetate, 0.5-10% of diethylene glycol fatty acid ester, 0.1-10% of shaping thickening agent and the balance deionized water to prepare cosmetic components, and preparing the cosmetics according to a preparation process. The method has the advantages that prepared cosmetics can effectively inhibit the growth the bacteria and fungi, is safe, mild and free of toxic and side effect, has multiple effects of resisting bacteria, diminishing inflammation, moisturizing the skin and preventing ultraviolet and can slow down skin aging.

The invention relates to the G test detection technical field, and particularly discloses a method for eliminating false positive interference of a G test, that is to say, a buffer solution containing a divalent soluble metal salt and Tris is prepared and is mixed with patient plasma or serum containing an interfering substance to prepare a detection sample, and the detection sample is subjected to the G test. With the use of the method, when the patient plasma/serum containing a nonspecific tachypleus amebocyte lysate is detected, the interference effect can be eliminated, the beta-G concentration in the patient plasma/serum is accurately detected, and patient mental and economic burdens caused by clinical misjudgment are greatly reduced.

The invention relates to a sensitive and accurate method for measuring the bacterial endotoxin content in injection grade granulesten, belonging to the field for detecting the bacterial endotoxin as an auxiliary material for a drug. In the method, the bacterial endotoxin content in the injection grade granulesten is measured by utilizing the developing substrate method of TAL (Tachypleus Amebocyte Lysate); the granulesten is accurately weighed; a 10mg/ml granulesten solution is prepared by utilizing the water for inspecting the bacterial endotoxin and is diluted to each dilution grade not exceeding the maximum effective dilution factor; the result of an interference test indicates that the 10mg/ml granulesten solution which is diluted by more than three times has no interference action on the measurement for the quantitive endotoxin of the developing substrate TAL; and the value of the bacterial endotoxin in the granulesten can be calculated by utilizing the dilution grade. The method provides a method for controlling and measuring the bacterial endotoxin for the production and scientific research of the injection grade granulesten.

The invention discloses a method for detecting the endotoxin content of a liquid. According to the method, the concentration of endotoxin in the liquid to be detected is learned by detecting the electrochemical response of an electrode in electrolyte after the liquid to be detected and a restructuring limulus factor C are mixed and a contact reaction is performed on the mixture and a peptide modified electrode; when the restructuring limulus factor C is prepared, the restructuring limulus factor C is recombined and is expressed by adopting a means of genetic engineering. Through the scheme, a result is obtained directly by an electrochemical technology; the interference of color samples in the conventional methods, such as a colorimetric method, is avoided; the using of tachypleus amebocyte lysate is avoided, so that the detection cost can be reduced, and moreover, the ecological pressure is lightened to a certain degree; the method specifically reacts to the endotoxin, the interference of factor G bypass in the conventional tachypleus amebocyte lysate reaction is avoided; meanwhile, the genetic engineering method is used, so that the uniformity among reagent batches in the detection method is guaranteed, and the industrial production is facilitated.

The invention discloses a method for detecting endotoxin in a Shenmai injection, and belongs to the field of medicines, and the method comprises the following steps: (1) determination of endotoxin limit value L; (2) sensitivity check of tachypleus amebocyte lysate; (3) interference test pre-testing for determining that a to-be-tested sample is diluted by a factor of 8; (4) interference tests of the to-be-tested sample for determining that non-interfere dilution factor is 32; and (5) detecting of the bacterial endotoxin in the to-be-tested sample. The method is rapid and sensitive. After determining the endotoxin limit value L and the dilution factor, the to-be-tested sample can be directly tested, no test is required to determine the dilution factor, while the detection accuracy is ensured, the inspection cost is reduced, inspection efficiency is improved, and production process monitoring and inspection work are convenient.

The invention provides a method for artificially breeding tachypleus tridentatus. The method for artificially breeding the adult tachypleus tridentatus comprises the following steps: selecting land, at a distance of 50-100 meters to the shore, near a sea area of a subtropical area as a farm; building breeding ponds, dividing each breeding pond into multiple breeding areas, forming channels betweenthe adjacent breeding areas, and dividing each breeding area with corresponding partition nets into multiple breeding units; controlling the water depth in the breeding ponds to be 1.5-1.6 meters; placing 2-4 tachypleus tridentatus into each breeding unit, and stocking ditrema temmincki and/or siganus in the breeding ponds; and feeding shellfish meat or small raw fishes into the breeding ponds every day. According to the method for artificially breeding the tachypleus tridentatus, the tachypleus tridentatus is dispersed into the breeding ponds to avoid excessive gathering, so that death rateincrease due to excessive local density is avoided; the method for artificially breeding the tachypleus tridentatus is suitable for blood collection for production of tachypleus amebocyte lysates, andespecially, repeated catches or catch missing is avoided; a breeding space is fully used, so that maximum of breeding benefits is achieved; and ecological breeding is achieved.

The invention discloses a detection method of remimazolam hydrobromide bacterial endotoxin. The detection method comprises the following steps: rechecking the sensitivity of a tachypleus amebocyte lysate; determining the limit value and the minimum dilution concentration of the remimazolam hydrobromide bacterial endotoxin; performing an interference experiment to confirm the concentration of a test sample without interference, wherein in the experiment, the test sample is dissolved by using an aqueous polysorbate-80 solution with a concentration of 10-30 mg/mL, and diluted by using water for bacterial endotoxin detection to obtain a test sample solution; and detecting the remimazolam hydrobromide bacterial endotoxin by dissolving a remimazolam hydrobromide test sample via the aqueous polysorbate-80 solution with the concentration of 10-30 mg/mL, and diluting the test sample to an effective concentration without interference by using water for bacterial endotoxin detection before conduction of detection on the bacterial endotoxin. According to the method, the aqueous polysorbate-80 solution with a certain concentration is used for dissolving the sample, so the interference effect ofthe test sample on bacterial endotoxin can be eliminated; and the method is simple, convenient, easy to operate and high in practicability.

The invention relates to a plasma diluent kit for bacterial endotoxin gel process detection of blood plasma and a use method thereof. The invention aims to solve the problems of easy influence of heparin sodium, sodium citrate and other anticoagulants in blood plasma and protein in blood plasma on the gel process, complex operation of the turbidimetric process, and expensive equipment. The kit comprises a diluent A, a diluent B and a diluent C. The kit adopts different diluents for dilution of the blood plasma of different anticoagulants, targetedly eliminates the interference of anticoagulants on tachypleus amebocyte lysate gel reaction, also can eliminate protein in blood plasma, and prevent the interference of protein. Also the dilution ratio is smaller, and a dilution ratio of 8 and conventional tachypleus amebocyte lysate sensitivity can achieve detection. The kit provided by the invention can be applied to the plasma endotoxin detection field.

The invention discloses a method for detecting endotoxin in a Danshen injection, and belongs to the field of medicines, and the method comprises the following steps: (1) determination of endotoxin limit value L; (2) sensitivity check of tachypleus amebocyte lysate; (3) interference test pre-testing for determining that a to-be-tested sample is diluted by a factor of 20; (4) interference tests of the to-be-tested sample for determining that non-interfere dilution factor is 80; and (5) detecting of the bacterial endotoxin in the to-be-tested sample. The method is rapid and sensitive. After determining the endotoxin limit value L and the dilution factor, the to-be-tested sample can be directly tested, no test is required to determine the dilution factor, while the detection accuracy is ensured, the inspection cost is reduced, the inspection efficiency is improved, and production process monitoring and inspection work are convenient.