113 results about "Beta d glucan" patented technology

The invention discloses a radiation-resistant and moisture-retention sun-blocking skin-care product, which contains 2-5 wt% of oat beta-glucan. The radiation-resistant and moisture-retention sun-blocking skin-care product provided by the invention can be used to raise radiation resistance and immunity of the whole skin and construct healthy skin, has an anti-allergic effect, can effectively prevent/alleviate various inflammation and symptoms such as erythema, and can preserve moisture, minimize fine lines and smoothen skin.

The invention discloses a hyaluronic acid dressing and a preparation method thereof. The dressing comprises the following components in percentage by mass: 0.01-0.3% of medium-molecular-weight hyaluronic acid, 0.01-0.5% of small-molecular-weight hyaluronic acid, 0.03-1% of allantoin, 0.1-1% of Carbomer, 2-5% of sorbitol, 0.2-1% of trehalose, 0.7-2.5% of witch hazel distillate, 0.1-0.5% of D-panthenol, 0.05-0.15% of alpha-bisabolol, 0.2-1.0% of oat glucan, 2-10% of glycerinum, 0.06-1% of water-soluble silicon wax, 0.05-0.9% of an antalkali, 0.004-0.2% of 2-methyl-4-isothiazoline-3-one, 0.05-0.4% of phenoxyethanol, 0.005-0.05% of pentanediol, 0.01-0.06% of octylene glycol, 0.025-0.5% of methylparaben and the balance of water. The dressing disclosed by the invention is capable of permeating into the deep layers of the skin to remove excessive free radicals, promoting the generations of blood vessels, and accelerating the union of wounds, in particular, acnes, eczematous dermatitis, photorejuvenation postoperation repair and the like.

The invention provides a preparation method of allergen-free oat beta-glucan. The preparation method comprises the following steps: (1) microwave drying; (2) carbon dioxide supercritical extraction; (3) amylase hydrolysis; (4) glucoamylase hydrolysis; (5) alkali extraction; (6) isoelectric point protein precipitation; (7) centrifugal separation to collect the supernate; (8) protease hydrolysis; (9) flocculant precipitation; (10) centrifugal separation to collect the supernate; (11) membrane separation; (12) vacuum condensation; (13) alcohol precipitation; (14) centrifugal separation to collect the precipitate; (15) vacuum drying. The oat beta-glucan extracted from oat bran does not contain any allergen, has the advantage of high purity, and has a specific molecular weight.

The invention provides a method for extracting beta-D-glucan from cell walls of candida utilis. The method includes steps of acquiring cells of the candida utilis; carrying out induction autolysis on the cells of the candida utilis; carrying out high-temperature digestion; ultrasonically breaking the walls; carrying out high-temperature extraction; carrying out degreasing; carrying out enzymatic hydrolysis to remove proteins, to be more specific, preparing suspension from degreased crude glucan by the aid of phosphoric acid buffer solution, carrying out enzymatic hydrolysis, carrying out reaction, then washing reaction products by the aid of water, centrifuging the reaction products, adding water into the reaction products, sufficiently uniformly mixing the reaction products and the water with one another to obtain mixtures, then heating the mixtures, deactivating enzymes, centrifuging the mixtures and freezing and drying the mixtures to obtain beta-D-glucan products. The method has the advantages that product degradation effects due to the traditional methods implemented by the aid of acid and alkali reagents can be prevented, and the method for extracting beta-glucan can be carried out under mild conditions; potato juice without the proteins is used as a culture medium for the candida utilis, accordingly, environmental pollution due to the discarded potato juice can be abated, and the method has important significance on environmental protection.

Objects of the present invention are to provide a DNA fragment encoding a limulus-derived pro-clotting enzyme, a virus harboring the DNA fragment, a cell harboring the virus, a method of producing the pro-clotting enzyme by use of the cell, and means for assaying an endotoxin or (1→3)-β-D-glucan employing the enzyme, wherein these elements are capable of producing an endotoxin or (1→3)-β-D-glucan assay reagent of satisfactory quality, steadily, at low cost, and on a large scale. In the present invention, for example, a DNA fragment encoding a protein having an amino acid sequence defined by SEQ ID NO: 4 is selected as a nucleic acid fragment encoding a limulus-derived pro-clotting enzyme, and the corresponding recombinant pro-clotting enzyme. Use of the enzyme can provide a high-sensitivity method and kit for detecting (1→3)-β-D-glucan and an endotoxin, utilizing a cascade reaction system in a horseshoe crab lysate.

The invention relates to a developing-process fungus 1,3-beta-D-glucan detection kit for human body fluid. The developing-process fungus 1,3-beta-D-glucan detection kit comprises a reaction main agent, a main agent compound solution, a sample treatment solution, heat-source-free water, a standard product and a quality control product, wherein the reaction main agent takes horseshoe crab blood cells as a main raw material and contains G factors, coagulase, coagulase zymogen and a polypeptide developing substrate; the polypeptide developing substrate is synthesized tripeptide or tetrapeptide with a Gly-Arg tail end connected with a PNA; the polypeptide developing substrate is subjected to enzyme digestion by adopting the coagulase; after the free paranitroaniline (PNA) is generated, a microplate reader is used for directly detecting so that a detection route is shortened and the cost is reduced; the microplate reader is used for carrying out a velocity-method enzyme kinetics detection method so that the sensitivity is relatively high when being compared with a nephelometry detection method; and the reaction main agent is not easily interfered by protein in a body fluid sample and medicines to generate non-specific turbidity, so that the probability of a false positive detection result is reduced and the detection accuracy is relatively high.

The invention discloses functional bread with beta-glucan. The bread is made from wheat flour, beta-glucan, pure water, yeast, sugar, salt and oil which are mixed in a certain ratio, and is made after stirring, mixing, fermentation, shaping, secondary fermentation and baking. beta-glucan is water-soluble fiber with the role of reducing cholesterin, reducing blood glucose and strengthening immune function and an ideal additive of bread production and has higher application value in actual production. The invention has the advantages of soft flavor, being rich in elasticity, being refreshing and not sticking, smell of baking products, the plump shape, the smooth surface, softness and elasticity, the even and fine bread, the swelling volume, the strong preservation ability and extending about 85 percent of shelf service life than that of the ordinary bread.

The invention relates to a chitosan containing antibacterial foaming agent for gynecology and a preparation method thereof, and belongs to the medicine field. The foaming agent is prepared from following raw materials in parts by weight: 0.5 to 2 parts of chitosan quaternary ammonium salt, 1 to 5 parts of chitosan, 0.5 to 2 parts of sodium hyaluronate, 0.5 to 1 part of 1,3-butylene glycol, 0.01 to1 part of oat beta-glucan, 0.2 to 2 parts of natural lactic acid, 2 to 5 parts of glycerin, 0.04 to 1 part of foaming agent, and 75 to 105 parts of purified water. The raw materials can generate a prominent synergistic effect on preventing pathogenic bacteria of colpitis. The foaming agent has a good antibacterial effect. Moreover, the irritation on vaginas is little, and the foaming agent is suitable for the treatments on gynecologic inflammation such as colpitis, cervicitis, and the like, and can be used to repair wounds of vagina mucosa.

The invention relates to an improved body fluid color-developing fungi 1,3-beta-D-glucan detection kit, which comprises a reaction main-agent, a main agent combination solution, a sample treatment fluid, sterile water, a standard substance and a quality control material. With Tachypleus tridentatus Leach or Limulus polyphemus Linnaeus hemocyte lysate as a main raw material, the reaction main-agent contains G factor, B factor, C factor, clottable protein and a polypeptide chromogenic substrate. By a new preparation process, the reaction main-agent has characteristics of high stability and small intra-assay and inter-assay difference. The main agent combination solution adopts a new formula. After the main agent combination solution is mixed with the reaction main-agent, Tachypleus Amebocyte Lysate endotoxin reaction branch can be effectively shielded. According to the kit, rate-method enzyme kinetics detection is carried out by ELIASA. The detection speed is fast, and anti-interference performance is strong. In addition, the preparation technology is simple, and the product has stronger stability and higher specificity and sensitivity.

The invention discloses a yeast beta-D-glucan derivative and a preparation method and an application thereof. The yeast beta-D-glucan derivative is obtained by substituting the hydroxyl of a yeast beta-D-glucan molecule with a sulfate group, wherein the degree of substitution of the sulfate group is 0.28-0.32 or 0.40-0.51. The invention further provides a method for preparing the yeast beta-D-dextran derivative, which comprises the following steps: dissolving yeast beta-D-glucan into an organic solvent and adding an esterifying agent for esterification reaction to obtain the yeast beta-D-dextran derivative. The contrastive analysis of a scanning electron microscopy discovers that the sulfated and modified yeast beta-D-glucan derivative is in a grid shape and has obvious grooves, the specific surface area is obviously increased, and the adsorption capacity is remarkably enhanced. An adsorption effect test shows that the adsorption quantity of zearalenone by the yeast beta-D-glucan derivative reaches 18.6528 mug/mg and is remarkably higher than that of the zearalenone by a yeast cell wall and the yeast beta-D-glucan.

The invention relates to a sulphating method for improving water solubility of yeast beta-D-glucan. The method comprises steps as follows: 1) taking the yeast beta-D-glucan as a raw material, dissolving the yeast beta-D-glucan in a dimethyl sulfoxide solution containing urea, and obtaining a solution a after stirring and dissolution; 2) placing the solution a in an ice-bath, still placing the solution a in the ice-bath after ultrasonic treatment, and slowly dropwise adding a DMSO (dimethylsulfoxide) solution containing H2SO4 while stirring to obtain a solution b; 3) placing the solution b in an oil-bath constant-temperature vibrator for an esterification reaction, quickly cooling an esterified solution to the room temperature, adding deionized water, performing microfiltration by the aid of a polyester film after dilution, and then sequentially performing ultrafiltration treatment, concentration and drying to obtain the sulphated yeast beta-D-glucan. The sulphating method is combined with ultrasound, and the prepared yeast beta-D-glucan has the advantages of good solubility, high production safety, wide application range, capability of effectively enhancing body immune functions and the like.

The invention relates to the technical field of detection of 1-3-beta-D glucan, and particularly discloses a simplified fungus 1-3-beta-D glucan detection kit. The kit comprises a kit body, an integrated reagent filling and reaction device, a reaction main agent, a main agent reconstitution fluid, a sample treating fluid and a quality control product; the integrated reagent filling and reaction device is composed of a reaction cup, a rear bin, a front bin and a push rod; and an integrated detection device can be formed by adding the reaction main agent, the main agent reconstitution solution and the sample treating fluid into the reaction cup, the rear bin and the front bin separately. The experiment operation is convenient, experiment tools are reduced, professionals are not needed, experiments are simple and rapid, and the detection accuracy is higher.