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48 results about "Horseshoe crab" patented technology

Horseshoe crabs are marine and brackish water arthropods of the family Limulidae, suborder Xiphosurida, and order Xiphosura. Their popular name is a misnomer, for they are not true crabs. Horseshoe crabs live primarily in and around shallow coastal waters on soft sandy or muddy bottoms. They tend to spawn in the intertidal zone at spring high tides. They are commonly eaten in Asia, and used as fishing bait, in fertilizer and in science (especially Limulus amebocyte lysate). In recent years, population declines have occurred as a consequence of coastal habitat destruction and overharvesting. Tetrodotoxin may be present in one horseshoe crab species, Carcinoscorpius rotundicauda.

Artificial breeding and culturing method for horseshoe crabs

InactiveCN102487853ASolving Seedling ProblemsEfficient foragingClimate change adaptationPisciculture and aquariaOysterHorseshoe crab
An artificial breeding and culturing method for horseshoe crabs includes: mixing eggs of horseshoe crabs with bodily fluid containing sperms, and allowing the mixture to stand until fertilization; putting the fertilized eggs into filtered seawater to be incubated, and filling oxygen until the eggs are incubated into one-year-old horseshoe crabs; breeding the one-year-old horseshoe crabs at the temperature of from 28 DEG C to 30 DEG C until the crabs slough and develop into two-year-old horseshoe crabs; breeding the two-year-old horseshoe crabs at the temperature of from 20 DEG C to 28 DEG C, and using an air pump to fill oxygen; taking out the two-year-old horseshoe crabs and placing into another container containing bait regularly each day, changing the filtered seawater once a day untilthe crabs are bred into five-year-old crabs; breeding the horseshoe crabs which are five years old or of different ages at the temperature of from 20 DEG C to 28 DEG C, and filling oxygen; taking outthe crabs and breeding in another container containing bait regularly each day, and changing the filtered seawater once a day; and taking out the crabs in breeding and breeding in a container or cement pit with oyster fragments until the crabs are bred into adult horseshoe crabs.
Owner:洪水根

Pro-clotting enzyme, and method for detection of endotoxin or (1-3)-beta-d-glucan using the same

Objects of the present invention are to provide a DNA fragment encoding a limulus-derived pro-clotting enzyme, a virus harboring the DNA fragment, a cell harboring the virus, a method of producing the pro-clotting enzyme by use of the cell, and means for assaying an endotoxin or (1→3)-β-D-glucan employing the enzyme, wherein these elements are capable of producing an endotoxin or (1→3)-β-D-glucan assay reagent of satisfactory quality, steadily, at low cost, and on a large scale. In the present invention, for example, a DNA fragment encoding a protein having an amino acid sequence defined by SEQ ID NO: 4 is selected as a nucleic acid fragment encoding a limulus-derived pro-clotting enzyme, and the corresponding recombinant pro-clotting enzyme. Use of the enzyme can provide a high-sensitivity method and kit for detecting (1→3)-β-D-glucan and an endotoxin, utilizing a cascade reaction system in a horseshoe crab lysate.
Owner:SEIKAGAKU KOGYO CO LTD

Method for largely and quick extracting tachyplesin peptide

The invention relates to a method for largely and quickly extracting tachyplesin peptide. The method comprises the following steps: (1) collecting horseshoe crab blood cells for reservation, suspending the collecting horseshoe crab blood cells in Tris-HCl and NaCl buffer solution homogenate, performing centrifugation for removing supernatant liquor, and washing sedimentation with the same buffer solution twice; (2) depositing, adding acid liquor homogenate according to the ratio of solid to liquid of 1: (6.5-8), performing centrifugation for collecting supernatant liquor, and re-extracting the sedimentation for three times by using acid liquor to obtain acid extraction liquid; (3) adjusting the pH of the acid extraction liquid to 6.5 by using NaOH, performing centrifugation to remove sedimentation, and performing filtration by use a 0.1-0.22 [Mu]m film to obtain supernatant liquor; (4) performing boiling water bath processing on the supernatant liquor, performing centrifugation to remove sedimentation, collecting supernatant liquor, performing filtration by use the 0.1.22 [Mu]m film to obtain supernatant liquor; (5) performing processing on ultrafiltration membrane with the molecular weight cut off of 10 KD; (6) performing processing on ultrafiltration membrane with molecular weight cut off of 3 KD; (7) performing desalination processing; and (8) performing freeze drying on the obtained product to be made into dry powder. The tachyplesin peptide obtained by using the method is high in yield and purity; the process is simplified; the time is greatly shortened; the cost is greatly reduced; and the throughout is increased significantly.
Owner:BEIHAI SINLON BIOTECH CO LTD

Traditional Chinese medicine (TCM) composition and application thereof

The invention belongs to the field of traditional Chinese medicines (TCMs) and discloses a TCM composition and application thereof. The composition comprises the following TCMs in parts by weight: 0.11-97.0 parts of musk, 10.0-50.0 parts of gypsum, 0.05-10.0 parts of borneol, 5.0-15.0 parts of pseudo-ginseng, 4.5-9.0 parts of cuttlebone, 3.0-9.0 parts of silkworm cocoons, 3.0-6.0 parts of human urine sediment, 4.0-80.0 parts of calcined gypsum, 0.5-25.0 parts of pearls, 2.0-12.0 parts of frankincense, 2.0-12.0 parts of myrrh, 1.0-10.0 parts of sanguis draconis, 1.0-10.0 parts of rhizoma coptidis, 3.0-20.0 parts of horseshoe crab shells, 0.2-2.0 parts of dragon bones, 9.0-30.0 parts of oysters and 0.5-15.0 parts of carbonized human hair. The TCM composition has the functions of clearing away heat and toxic materials, activating blood circulation to dissipate blood stasis, carrying out sterilization to diminish inflammation, relieving pains, diminishing swelling and promoting granulation, inducing astringency and stopping slippage and astringing dampness and carrying out astringency. The TCM composition can be used for preparing the medicines for treating bedsore, ecthyma, scrofula sore, hemorrhoids, burns, scalds, tympanitis or diabetic foot.
Owner:王广志

Developing-process fungus 1,3-beta-D-glucan detection kit for human body fluid

The invention relates to a developing-process fungus 1,3-beta-D-glucan detection kit for human body fluid. The developing-process fungus 1,3-beta-D-glucan detection kit comprises a reaction main agent, a main agent compound solution, a sample treatment solution, heat-source-free water, a standard product and a quality control product, wherein the reaction main agent takes horseshoe crab blood cells as a main raw material and contains G factors, coagulase, coagulase zymogen and a polypeptide developing substrate; the polypeptide developing substrate is synthesized tripeptide or tetrapeptide with a Gly-Arg tail end connected with a PNA; the polypeptide developing substrate is subjected to enzyme digestion by adopting the coagulase; after the free paranitroaniline (PNA) is generated, a microplate reader is used for directly detecting so that a detection route is shortened and the cost is reduced; the microplate reader is used for carrying out a velocity-method enzyme kinetics detection method so that the sensitivity is relatively high when being compared with a nephelometry detection method; and the reaction main agent is not easily interfered by protein in a body fluid sample and medicines to generate non-specific turbidity, so that the probability of a false positive detection result is reduced and the detection accuracy is relatively high.
Owner:DYNAMIKER BIOTECH TIANJIN

Horseshoe crab anti-endotoxin factor analogue peptide molecule, synthetic method and use thereof

The invention discloses an analog peptide molecule of antiendotoxin factor of Limulida to kill bacteria and treat endotoxin blood symptom, which applies computer molecular analog technique to design the following 6 groups of analog peptides antiendotoxin factor of Limulida based on GOR method with TALF functional area position as mould: RX1 NPT X2 KRLK X3 KYKGK X3 WCP as the first group, X4 INPT X2 KR X1 X4 X3 KYKGKFW as the second group, INPT X2 KRLKW X4 YKGR X3 X3 CP as the third group, PT X2 KR X1 KX3 KYKGKFWCP as the forth group, K X2 NPTVKR X1 K X3 RYKGKF as the fifth group, NPX5 VKR X1 K X3 RYKGKF as the sixth group, wherein six groups are synthesized into the peptide through solid phased peptide synthetic method by self-synthesizing and manual synthesizing, which produces the peptide with sterilizing and / or neutralizing LPS activity.
Owner:THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA

Recombinant Factor C and method for producing the same, and method for measuring endotoxin

To provide a method for producing a horseshoe crab recombinant Factor C. The horseshoe crab recombinant Factor C is produced through expression thereof by use of mammalian cells such as CHO DG44 and HEK293 as host cells.
Owner:SEIKAGAKU KOGYO CO LTD

Sugar-free traditional Chinese medicinal buccal tablet for treating sphagitis

The invention discloses a sugar-free traditional Chinese medicinal buccal tablet for treating sphagitis. The buccal tablet is characterized by being prepared from the following traditional Chinese medicinal raw materials in parts by weight: 5-15 parts of licorice root, 10-30 parts of horseshoe crab shell, 2-8 parts of umbellate rock jasmine, 5-15 parts of radix scrophulariae, 5-15 parts of radix ophiopogonis, 5-15 parts of dried dendrobe, 5-15 parts of pachymenia carnosa and 15-40 parts of additives. By adopting a treatment method of combining traditional Chinese medicinal materials and probiotics, the buccal tablet can be used for avoiding the defects of poor curative effect, easily generated drug resistance and the like caused by singly using traditional Chinese medicinal materials or probiotics on treatment, has remarkable curative effect on treatment of sphagitis, has the advantages of no side effect, good taste and no sugar, is convenient to take, is suitable for patients with diabetes mellitus, and cannot cause drug resistance when taken for a long time.
Owner:王正琦

Method for producing factor G derived from horseshoe crab

The invention provides a virus harboring a DNA encoding a subunit of limulus-derived factor G, the virus being capable of mass-producing a (1→3)-β-D-glucan assay reagent of satisfactory quality, steadily and at low cost, a cell harboring the virus, and a method of producing factor G by use of the cell.
Owner:SEIKAGAKU KOGYO CO LTD

Method for obtaining natural horseshoe crab fertilized eggs by building pool bottom overhead, laying sand and conducting partition

The invention discloses a method for obtaining natural horseshoe crab fertilized eggs by building a pool bottom overhead, laying sand and conducting partition. The method is characterized by including the steps of firstly, selecting parent horseshoe crabs, wherein three or more pairs of parent horseshoe crabs are selected as the parents in the mating season each year; secondly, refitting a parent horseshoe crab pool indoors, wherein the pool bottom of a breeding pool is built overhead through a wood frame, a 60-mesh bolting-silk net is laid on the pool bottom, an oxygen pipe used for inflation is arranged in the overhead wood frame, and sand is laid on an egg laying area; thirdly, breeding the parent horseshoe crabs indoors, wherein the parent horseshoe crabs selected in the first step are placed in the parent horseshoe crab pool refitted in the second step, and indoor inflation breeding is conducted; fourthly, making the parent horseshoe crabs lay eggs and release semens, wherein the parent horseshoe crabs can lay eggs and release semens in 2 days to 10 days after entering the breeding season to form fertilized eggs; fifthly, sieving and washing the fertilized eggs; wherein after the fertilized eggs are hardened, the fertilized eggs and sand are dug out, elutriation is conducted through a screen with the diameter of 2 mm, all the sand is leaked out, and the fertilized eggs are left. Different from a method for obtaining the fertilized eggs by killing horseshoe crabs, the method has the advantages that a large number of high-quality fertilized eggs can be obtained, and the method is easy and convenient to operate, efficient, highly feasible and the like.
Owner:GUANGXI ACADEMY OF FISHERY SCI

Herpes zoster treatment Chinese patent medicine

The present invention discloses a herpes zoster treatment Chinese patent medicine, which is prepared from the following raw materials, by weight: 18-29 parts of medicinal morinda root, 15-27 parts of tatarinow sweetflag rhizome, 9-17 parts of Chinese pulsatilla root, 10-19 parts of horseshoe crab carapace, 15-20 parts of sharpleaf uncaria stem with hooks, 19-27 parts of rheum, 15-19 parts of sapindus mukurossi, 12-19 parts of snake slough, and 10-16 parts of isatis tinctoris. The herpes zoster treatment Chinese patent medicine has the following advantages that: the medicine is prepared from Chinese herbs, has effects of no toxic-side effects, anti-inflammation, pain relieving, detoxification, sterilization, heat clearing and blood cooling, has characteristics of adequate raw materials, simple production process, low price and convenient use, and is the external application specially good effect medicine for treating skin herpes zoster, and clinical case treatment results verify that an effective rate is 100%, a cure rate is 91.7%, and no pigmentation and sequel is generated after healing. In addition, the herpes zoster treatment Chinese patent medicine provides a certain treatment effect for other skin herpes and papule.
Owner:徐茂恺

Method for preparing kynurenine from horseshoe crab tails

The invention discloses a method for preparing kynurenine from horseshoe crab tails.The method comprises the following steps of preparation of dry horseshoe crab tail powder, preparation of horseshoe crab tail crude extract, primary separation and purification of the horseshoe crab tail crude extract, separation and purification of a component A8 and separation and purification of components A8-24, wherein separation and purification of the components A8-24 comprise the steps that an appropriate amount of components A8-24 are taken, methyl alcohol is added for dissolution, and normal phase column separation is conducted; 50 mg of samples are taken and stirred with 150 mg of silica gel powder, 5 g of silica gel is saturated with petroleum ether and then subjected to column packing, after the the samples are packed in a column, elution is conducted with petroleum ether and acetone, when the ratio of petroleum ether to acetone is 1:10, fractions are collected, the number of the fractions is 60, the fractions from the 25 tube to the 40 tube are merged, after the the fractions are merged, vacuum concentration is conducted, and after solvent is dried out, kynurenine is obtained.According to the method for preparing kynurenine from the horseshoe crab tails, kynurenine is separated from the horseshoe crab tails for the first time, and kynurenine serving as the active ingredient of the horseshoe crab tails has great significance in secondary development of horseshoe crab tails.
Owner:JIMEI UNIV

Method for accelerating artificial breeding speed of horseshoe crabs of China

The invention relates to a method for accelerating the embryonic development speed of horseshoe crabs of China by controlling the environmental factors. The method is characterized by comprising the steps of culturing fertilized eggs produced after copulation of the horseshoe crabs of China in a rectangular mesh basket under the conditions that the water temperature is 27.5-31 DEG C, the pH value is 7.8-8.0, the dissolved oxygen is 6.3-7.0, the illumination is 60-3000 lx and the salinity is 32-35 per thousand; putting the rectangular mesh basket into a culture pond, wherein the water depth of the culture pond is 0.5-2.0m. After the method for accelerating the embryonic development speed of the horseshoe crabs of China by controlling the environmental factors is used for culturing the horseshoe crabs of China, the horseshoe crabs of China generally begin to take off the membrane and are incubated from about the thirtieth day, so that time is shorter; therefore, the breeding time is shortened, the survival rate is improved, and the breeding cost is saved.
Owner:QINZHOU UNIV

Restoration culture method for injured Chinese horseshoe crab

ActiveCN111316956AClear water quality indicatorsGuaranteed wound infectionClimate change adaptationAnimal feeding stuffOXALIC ACID DIHYDRATEFiltration
The invention discloses a restoration culture method for an injured Chinese horseshoe crab. The method comprises the following steps: cleaning the internal wall and bottom of a pool with an garlic detergent, sprinkling an oxalic acid solution for disinfection, and flushing the repair pool with fresh water on next day until no oxalic acid solution remains; laying a sandy bottom of 20cm-30cm, carrying out soaking disinfection for 30min with 10ppm potassium permanganate, and flushing the sandy bottom with fresh water until no potassium permanganate remains; subjecting the repair pool to sun curing for 24 hours, and adding fresh seawater subjected to sand filtration, dark and ultraviolet light disinfecting treatment; uniformly laying gas pipes to continuously inflate, so as to keep dissolved oxygen to be 5mg / L or more; rinsing out the injured Chinese horseshoe crab with the seawater in the repair pool, and carrying out disinfecting for 15min; placing the treated injured Chinese horseshoe crab into the repair pool, and carrying out culture with running water; lowering the water level to 15cm to 20cm every morning; feeding fresh waste fish or oyster meat in the afternoon of first severaldays, and cleaning remaining remnant bait after the injured Chinese horseshoe crab finishes food intake; adjusting a feeding amount at a later stage according to food intake conditions; and carryingout culture until rapid body contraction reduction is achieved through touch in water and the food intake conditions are stable.
Owner:广西海洋研究所有限责任公司

Application of tachyplesin peptides in preparation of medicine and healthcare products for preventing and treating pulmonary fibrosis

The invention discloses new application of tachyplesin peptides. The tachyplesin peptides can be used for preparing medicine and healthcare products for preventing and treating pulmonary fibrosis, used as the effective component of the medicine and healthcare products for preventing and treating pulmonary fibrosis, used for the prodrug modification of the medicine for preventing and treating pulmonary fibrosis or used along with the preventing and treating pulmonary fibrosis to treat the pulmonary fibrosis. The tachyplesin peptides are prepared by waste materials of horseshoe crab blood cell or horseshoe crab reagent production and can evidently inhibit the formation of the pulmonary fibrosis.
Owner:GUANGDONG MEDICAL UNIV

Buffer liquid for bacteria endotoxin horseshoe crab test detection method and process for preparing same

The buffering liquid for limulus test for detecting bacterian endotoxin is Tris-HCl buffering liquid containing phenol red in 0.005-0.1 wt% concentration. The preparation process includes weighing phenol red and Tris salt and stoving at 150-200 deg.c for 1-5 hr; adding no-endotoxin water and regulating pH to required value with concentrated hydrochloric acid; high pressure sterilization and no-bacteria preservation or freezing preservation. The buffering liquid is used in diluting tested sample and regulating and indicating sample pH value. The buffering liquid for limulus test for detecting bacterian endotoxin has no influence on the endotoxin in the detection of two kinds of endotoxin and coincides with the requirement for limulus test.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

A method for preparing kynuric acid from Limulus chinensis

The invention discloses a method for preparing kynurenine from horseshoe crab tails.The method comprises the following steps of preparation of dry horseshoe crab tail powder, preparation of horseshoe crab tail crude extract, primary separation and purification of the horseshoe crab tail crude extract, separation and purification of a component A8 and separation and purification of components A8-24, wherein separation and purification of the components A8-24 comprise the steps that an appropriate amount of components A8-24 are taken, methyl alcohol is added for dissolution, and normal phase column separation is conducted; 50 mg of samples are taken and stirred with 150 mg of silica gel powder, 5 g of silica gel is saturated with petroleum ether and then subjected to column packing, after the the samples are packed in a column, elution is conducted with petroleum ether and acetone, when the ratio of petroleum ether to acetone is 1:10, fractions are collected, the number of the fractions is 60, the fractions from the 25 tube to the 40 tube are merged, after the the fractions are merged, vacuum concentration is conducted, and after solvent is dried out, kynurenine is obtained.According to the method for preparing kynurenine from the horseshoe crab tails, kynurenine is separated from the horseshoe crab tails for the first time, and kynurenine serving as the active ingredient of the horseshoe crab tails has great significance in secondary development of horseshoe crab tails.
Owner:JIMEI UNIV

A method for obtaining natural fertilized eggs of Limulus japonicus by adopting the overhead sand-dressing partition at the bottom of the pool

The invention discloses a method for obtaining natural fertilized eggs of horseshoe crabs by adopting overhead sand-spreading partitions at the bottom of the pool, which is characterized in that it comprises the following steps: a. selection of pro-Limulus: in the breeding season of Limulus each year, select more than 3 pairs of pro-Limulus As a parent; b. Indoor modification of the horseshoe crab pool: use a wooden frame to lift the bottom of the breeding pool, spread 60-mesh silk screen, place the oxygen tube for inflation in the overhead wooden frame, and spread sand on the spawning area c. Cultivation of the pro-Limulus indoor: the pro-Limulus in step a is placed in step b to carry out indoor aerated culture; d. pro-Limulus lays eggs and ejaculates: when entering the breeding period, the pro-Limulus will lay eggs within 2-10d, Fertilization to form fertilized eggs; e. Screening and washing of fertilized eggs: After the fertilized eggs harden, dig out the fertilized eggs and sand, use a 2mm sieve to wash them, and drain all the sand, leaving fertilized eggs. The method of the present invention is different from the way of killing Limulus crabs to obtain fertilized eggs, and can obtain a large number of high-quality fertilized eggs. The present invention has the advantages of simple operation, high efficiency and easy implementation.
Owner:GUANGXI ACADEMY OF FISHERY SCI

Horseshoe crab antiviral combined extract, preparation method and application of horseshoe crab antiviral combined extract to disinfection products

The invention discloses a horseshoe crab antiviral combined extract, a preparation method and an application of the horseshoe crab antiviral combined extract to disinfection products. Two extracts with antiviral effects are respectively obtained from horseshoe crab serum and horseshoe crab blood cells, and the extracts have more obvious inhibition effects on viruses when being combined for use. The novel coronavirus resisting activity of a spray dressing formed by combining the two extracts reaches up to 99.51%, and the influenza A virus resisting activity is 42.58%. The two extracts can be extracted from waste materials obtained from horseshoe crab reagent production, so that the production waste materials are recycled, and biological resources can also be fully utilized while the production cost is reduced. The two antiviral extracts can be prepared to obtain dressings for nasal cavity and skin disinfection. Compared with traditional chemical disinfection products, the two antiviral extracts are safer, milder and more effective, have no toxic and side effects, and can avoid drug resistance and allergy phenomena of conventional disinfection products.
Owner:GENOBIO PHARM CO LTD +3

An anti-endotoxin coupled biomolecule and its preparation and application

The invention relates to an antiendotoxin coupling biological molecule. The coupling biological molecule is a compound shown in the formula Y-CLP-19-amPEG, wherein CLP-19 is a horseshoe crab antiendotoxin factor simulation peptide molecule, Y is selected from one of hydrogen, acyl group, an alkyl group of C1-C18, an aryl group, a benzyl group and an allyl group, and the molecular weight of amPEG is 500, 1000, 2000, 4000, 6000, 12000 or 20000. The direct antibacterial activity of CLP-19 obtained through the molecule and modified by PEG is better than or equivalent to that of unmodified CLP-19; the affinity of the modified CLP-19 and LPS is remarkably improved, a better LPS neutralizing characteristic is achieved, a remarkable progress is made compared with the CLP-19, and a novel sepsis treating candidate medicine is expected to be developed.
Owner:THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA

Method for producing factor G derived from horseshoe crab

The invention provides a virus harboring a DNA encoding a subunit of limulus-derived factor G, the virus being capable of mass-producing a (1→3)-β-D-glucan assay reagent of satisfactory quality, steadily and at low cost, a cell harboring the virus, and a method of producing factor G by use of the cell.
Owner:SEIKAGAKU KOGYO CO LTD

Artificial bait based on a peptide attractant found in horseshoe crab eggs

The present invention relates to a novel attractant for fish and mollusks isolated from the eggs of the horseshoe crab, Limulus polyphemus. The invention also relates to a method for attractant peptide extraction, separation, purification, quantitation, and characterization of function. In other aspects, the invention provides for the isolation of the attractant peptide from horseshoe crab eggs for use as “bait” for attracting fish and mollusks. More particularly, the invention relates to the use of biochemical approaches to characterize a peptide attractant, and provides for the creation of recombinant or synthetic attractant peptides, and their use.
Owner:UNIVERSITY OF DELAWARE

A kind of Chinese Limulus ssr primer set and application thereof

The present invention relates to the technical field of molecular markers, in particular to a Chinese horseshoe crab SSR primer set and its application. The present invention extracts DNA from Chinese horseshoe crabs, and after sequencing and assembling, designs corresponding SSR primers to target multiple Chinese horseshoe crabs in the Beibu Gulf region. Genomic DNA was used as the material, and the designed SSR primers were verified, and 164 pairs of SSR primers were obtained that could amplify in Chinese horseshoe crabs. Among them, 20 pairs of polymorphic SSR primers were selected for molecular genetic markers of Chinese horseshoe crabs, which can distinguish multiple Chinese horseshoe crabs in the Beibu Gulf area. The gene of horseshoe crab sample, cluster analysis of different species of horseshoe crab in the Beibu Gulf area, provides new ideas for future research on genetic breeding, gene mapping, identification of species kinship and protection of genetic resources of horseshoe crab in China.
Owner:BEIBU GULF UNIV

Preparation method, product and application of horseshoe crab peptide cream

The invention relates to a preparation method, a product and an application of horseshoe crab peptide cream. The preparation method of the horseshoe crab peptide cream comprises the steps as follows:1) meat liquids and eggs of horseshow crabs are collected and pulped by a colloid mill, and fine pulp is obtained; 2) animal protease and flavor enzyme are added to the fine pulp for hydrolysis, heating for enzyme deactivation is conducted after hydrolysis ends, and an enzymatic hydrolysate is obtained; and 3) the enzymatic hydrolysate is subjected to vacuum concentration and the horseshoe crab peptide cream is obtained. The horseshoe crab peptide cream prepared with the preparation method can be used as an attractant, the ingestion desire of laboratory rats can be increased, the food intake of the laboratory rats can be guaranteed, and the molding quality of animal models can be improved.
Owner:ZHEJIANG ACAD OF MEDICAL SCI

A kind of genetic engineering horseshoe crab blood g factor and its preparation method and application

The invention discloses a genetic engineering limulus blood G factor as well as a preparation method and an application thereof. According to the invention, nucleotide sequences of [alpha] subgroup and [beta] subgroup genes of the G factor undergo codon optimization, and optimized sequences are shown as SEQ ID NO:1 and SEQ ID NO:2; meanwhile, a prokaryotic expression vector of G factor gene subgroup optimized sequence is successfully constructed, and the prokaryotic expression vector is transformed into escherichia coli for efficient induced expression; expression protein is extracted and purified, and the two subgroups undergo effective fusion; and finally, bio-activity verification is implemented on target protein obtained from fusion. According to the invention, the [alpha] subgroup andthe [beta] subgroup, which achieve efficient expression in the escherichia coli, are successfully obtained, and the two subgroups are re-assembled, so that the G factor, which is relatively high in activity and purity, is obtained; the preparation method is simple in preparation process and low in cost; the G factor has a good biological function, the G factor can be used for producing a limulusreagent for detecting fungal infection and the G factor can reduce false positive; meanwhile, a demand on wild limulus resource can be greatly reduced; and the G factor has a broad application prospect.
Owner:GUANGDONG MEDICAL UNIV
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