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Method for preparing kynurenine from horseshoe crab tails

A technology of kynuric acid and Limulus tail, applied in the field of active medicine

Active Publication Date: 2016-07-27
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, it has not been reported that kynuric acid exists in Limulus sinensis

Method used

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  • Method for preparing kynurenine from horseshoe crab tails
  • Method for preparing kynurenine from horseshoe crab tails
  • Method for preparing kynurenine from horseshoe crab tails

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Embodiment Construction

[0033] The invention discloses a method for preparing kynuric acid from Limulus sinensis tail, comprising the following steps:

[0034] Step 1. Preparation of Limulus chinensis dry powder: Dry Limulus chinensis naturally in the sun, and pulverize it with a pulverizer at room temperature to obtain dry powder, put it in a sealed bag, and store it in a desiccator for later use;

[0035] Step 2. Preparation of Limulus chinensis in bold: Weigh 5 parts of Limulus chinensis that has been powdered, each 1000g, put them in five 2L triangular flasks respectively, add acetic acid according to the ratio of material to liquid 1:3:16 , ethyl acetate and methanol, sealed and placed in a shaker, extracted at 40°C and 150rpm for 48 hours, filtered with suction, and collected the filtrate; ethyl acetate and methanol) for the second extraction, under the same conditions (40°C, 150rpm) for 24h, suction filtration, and the filtrate was collected. The second extraction, the extraction conditions a...

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Abstract

The invention discloses a method for preparing kynurenine from horseshoe crab tails.The method comprises the following steps of preparation of dry horseshoe crab tail powder, preparation of horseshoe crab tail crude extract, primary separation and purification of the horseshoe crab tail crude extract, separation and purification of a component A8 and separation and purification of components A8-24, wherein separation and purification of the components A8-24 comprise the steps that an appropriate amount of components A8-24 are taken, methyl alcohol is added for dissolution, and normal phase column separation is conducted; 50 mg of samples are taken and stirred with 150 mg of silica gel powder, 5 g of silica gel is saturated with petroleum ether and then subjected to column packing, after the the samples are packed in a column, elution is conducted with petroleum ether and acetone, when the ratio of petroleum ether to acetone is 1:10, fractions are collected, the number of the fractions is 60, the fractions from the 25 tube to the 40 tube are merged, after the the fractions are merged, vacuum concentration is conducted, and after solvent is dried out, kynurenine is obtained.According to the method for preparing kynurenine from the horseshoe crab tails, kynurenine is separated from the horseshoe crab tails for the first time, and kynurenine serving as the active ingredient of the horseshoe crab tails has great significance in secondary development of horseshoe crab tails.

Description

technical field [0001] The invention relates to the technical field of active medicines, in particular to a method for preparing kynuric acid from Limulus sinensis. Background technique [0002] Kynurenine (Kyn) is one of the metabolites of the essential amino acid tryptophan (Trp). In addition to being used for protein synthesis, the Trp ingested by the human body is mainly metabolized in the liver, kidney, brain and other tissues and organs to produce a variety of biologically active molecules with physiological effects, such as Kyn, 5-hydroxytryptamine, quinolinic acid, etc. Kyn and its metabolites are closely related to a variety of neuropsychiatric diseases, renal failure, cataracts, and various chronic malignant diseases. Therefore, Kyn has important reference value for the diagnosis and treatment of related diseases, efficacy monitoring and pathogenesis research. In the prior art, it has not been reported that kynuric acid exists in Limulus sinensis. [0003] In vi...

Claims

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Application Information

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IPC IPC(8): C07D215/48
CPCC07D215/48
Inventor 李健苏文金刘鹏飞苏国成刘静雯王力
Owner JIMEI UNIV
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