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Bacteriotoxin quantitive detecting method-dynamic end-point method

A quantitative detection method and technology for bacterial endotoxins, which are applied in the measurement of color/spectral characteristics and the use of stored programs for program control. It can solve the problem of blindness in the selection of parameter endpoint time, and achieve the effect of good linearity and reasonable selection.

Active Publication Date: 2004-09-15
湛江安度斯生物有限公司
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AI Technical Summary

Problems solved by technology

[0014] The problem to be solved by the present invention is that the existing endpoint method technology has the defect of blindness in the selection of the important parameter endpoint time, and provides a new method for quantitative detection of bacterial endotoxin - dynamic endpoint method

Method used

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  • Bacteriotoxin quantitive detecting method-dynamic end-point method
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  • Bacteriotoxin quantitive detecting method-dynamic end-point method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0066] 1. Test material

[0067] Standard Endotoxin (CSE), Limulus Reagent (TAL), Bacterial Endotoxin Test Water (W);

[0068] The sample to be tested is the test product (S);

[0069] Photoelectric detector.

[0070] 2. Test methods and steps

[0071] 2.1 Determine the standard endotoxin concentration required to establish a standard curve. Such as: 0.3, 0.15, 0.075, 0.03Eu / ml, respectively recorded as E0.3, E0.15, E0.075, E0.03;

[0072] 2.2 Take 1 bottle (bottle) of CSE, dilute it to the required concentration with W according to the marked potency, and react with TAL on a photoelectric detector for 60 minutes; the photoelectric detector dynamically collects the reaction data (light intensity value) during the reaction process. .

[0073] 2.3 Analyze the reaction dynamic curve recorded and depicted by the photoelectric detector through the computer, determine a reasonable end time period, and use the computer to search for the best end time T in the end time period 0 ...

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Abstract

The invented testing method solves blindfold determination of important parameter-terminal hour in prior art, making selection of terminal hour possess rationality. Adopting photoelectric detecting instrument, the invention records, plots dynamic curve of reaction, and searches out optimal terminal hour by using computer. Then, end-point method is carried out for testing content of endotoxin in sample quantificationally by utilizing the said terminal hour. The method is applicable to horseshoe crab reagent in end-point method as well as reagents in dynamic turbidimetry or dynamic colorimetry.

Description

technical field [0001] The invention relates to a quantitative detection method for bacterial endotoxin. Background technique [0002] Bacterial endotoxin is a lipopolysaccharide component in the cell wall structure of gram-negative bacteria, has a variety of biological activities, and is a major pyrogenic substance. [0003] The reagent used for bacterial endotoxin detection is Limulus Reagent (TAL or LAL). TAL contains factor C, factor B, coagulase and coagulation protein that can be activated by very small amounts of bacterial endotoxin. Under suitable conditions (temperature, pH value and no interfering substances, etc.), bacterial endotoxin can cause TAL to produce agglutination reaction. [0004] The methodological classification of bacterial endotoxin testing is as follows: [0005] [0006] [0007] The principle of bacterial endotoxin quantitative detection method is to use a photoelectric detector to convert the turbidity / chromaticity change generated by t...

Claims

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Application Information

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IPC IPC(8): G01N21/25G06F9/06
Inventor 冯聚锦肖洪生熊向党
Owner 湛江安度斯生物有限公司
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