Method for obtaining natural horseshoe crab fertilized eggs by building pool bottom overhead, laying sand and conducting partition
A technology of fertilized eggs and sand partitions, applied in the field of natural fertilized eggs, can solve problems such as low efficiency, achieve improved quality, ensure stability, and be conducive to egg laying and ovulation.
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Embodiment 1
[0027] Embodiment 1: a kind of method of the present invention adopts overhead sanding partition at the bottom of the pool to obtain the natural fertilized egg of horseshoe crab, and it comprises the following steps:
[0028] a. Selection of pro-Limulus: At the beginning of June 2009, during the breeding season of Limulus in China, 2 pairs of pro-Limulus were selected as parents. After inspection, it was found that the ventral surface of the carapace was full, the body contained a large number of gametes, and the limbs were intact, strong and active; and the rear cover of the horseshoe crab was lifted, and a small amount of egg fluid was released by the female, and a small amount of egg fluid was released by the male. A small amount of semen comes out.
[0029] b. Indoor sanding and transformation of the Limulus pool: In order to further try the behavior of traditional cement pool sanding to obtain natural fertilized eggs, a 10m3 cement pool was selected to only sand 30cm, and...
Embodiment 2
[0035] Embodiment 2, a kind of method that adopts overhead sand-spreading partition at the bottom of the pool to obtain natural fertilized eggs of horseshoe crab of the present invention, it comprises the following steps:
[0036] a. Selection of pro-Limulus: At the beginning of June 2012, during the breeding season of Chinese Limulus, 5 pairs of pro-Limulus were selected as broods. After inspection, it was found that the ventral surface of the carapace was full, the body contained a large number of gametes, and the limbs were intact, strong and active; and the rear cover of the horseshoe crab was lifted, and a small amount of egg fluid was released by the female, and a small amount of egg fluid was released by the male. A small amount of semen comes out.
[0037] b. Indoor reconstruction of the horseshoe crab pool: use a wooden frame to lift the bottom of the 50m2 cement pool, the height of which is 12cm, spread 60-mesh sieve silk net, lay it flat and fix it, and put the infl...
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