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Method for establishing pancreatic cancer model

A pancreatic cancer, model technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, cells modified by introducing foreign genetic material, etc. The method is fast and efficient, and the operation is simple and fast.

Active Publication Date: 2015-12-09
成都豆麦科技有限公司
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  • Method for establishing pancreatic cancer model
  • Method for establishing pancreatic cancer model
  • Method for establishing pancreatic cancer model

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Embodiment Construction

[0030] The present invention will be further elaborated below in conjunction with the accompanying drawings and embodiments.

[0031] The method for establishing a pancreatic cancer model with tree shrews as the research object of the present invention, according to the oncogene and tumor suppressor gene with the highest mutation rate in human pancreatic cancer in the TCGA database, constructs a model that overexpresses one oncogene and simultaneously knocks down two tumor suppressor genes. Lentivirus, which is injected in situ in the head of the pancreas to induce the occurrence of pancreatic cancer.

[0032] The oncogene with the highest mutation rate in human pancreatic cancer overexpressed in the same lentiviral vector is Kras, and the tumor suppressor genes TP53 and CDKN2A are knocked down at the same time.

[0033] The tree shrew, which is closer to human relatives, is used as the object, and the lentivirus is injected into the pancreatic head of the tree shrew in situ t...

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Abstract

The invention relates to a method for establishing a pancreatic cancer model, and belongs to the technical field of the establishment of an animal model. According to the method, a slow virus for over-expressing one cancer gene and simultaneous knocking down two cancer suppressor genes is constructed according to cancer genes and cancer suppressor genes having the maximum mutation rate in human pancreatic cancer in TCGA database, and the slow virus is applied to the pancreas head of a tree shrew through orthotopic injection so as to induce pancreatic cancer of the tree shrew; and specifically, the method comprises the following steps: (1) construction of a slow virus vector; (2) virus packaging, titre determination and in vitro verification; (3) virus injection; and (4) monitoring and pathological investigation of the tree shrew. The method disclosed by the invention has the advantages that the method is short in inducing cycle, high and stable in morbidity, and simple and convenient in operation, and is capable of rapidly and effectively establishing a tree shrew pancreatic cancer model for simulating the genetic mechanism of human pancreatic cancer to the greatest extent; and the pancreatic cancer model is applicable to the research of the pathogenesis of human pancreatic cancer, the exploration of cancer therapy targets and the development of novel antineoplastic drugs, so as to offer a reference for the treatment of human pancreatic cancer.

Description

Technical field: [0001] The invention belongs to a method for establishing an animal model, and in particular relates to a method for inducing a tree shrew pancreatic cancer model by using a lentivirus to mediate the overexpression of an oncogene and the knockdown of a tumor suppressor gene. Background technique: [0002] Pancreatic cancer is a highly malignant tumor of the digestive tract that is difficult to diagnose and treat. Its morbidity and mortality have increased significantly in recent years, and its 5-year survival rate is <5%. It is one of the malignant tumors with the worst prognosis. . It is very important to study the mechanism of occurrence and development of pancreatic cancer, and to find effective detection and treatment methods, and tumor animal models are one of the important tools for studying the mechanism of tumor occurrence and development, exploring new therapeutic targets, new treatment methods and drug development . [0003] At present, animal...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/867C12N5/10A01K67/027
Inventor 赵旭东涂秋
Owner 成都豆麦科技有限公司
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