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Tree shrew hepatocyte immortalized cell line and its construction method and application

A technology for immortalized cell lines and construction methods, applied in the field of tree shrew liver cell immortalized cell lines and its construction, can solve the problems of time-consuming, long gene introduction or gene editing, etc. The effect of the acquisition method

Inactive Publication Date: 2017-07-11
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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Problems solved by technology

However, the liver is an organ at the end of differentiation. Even if primary hepatocytes are obtained through time-consuming and laborious operations, the cells will die after a few days of in vitro culture, making it difficult to perform time-consuming experiments such as gene introduction or gene editing. Research

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  • Tree shrew hepatocyte immortalized cell line and its construction method and application
  • Tree shrew hepatocyte immortalized cell line and its construction method and application
  • Tree shrew hepatocyte immortalized cell line and its construction method and application

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Embodiment Construction

[0029] The present invention will be further described in detail below in conjunction with embodiments.

[0030] Those skilled in the art will understand that the following examples are only used to illustrate the present invention and should not be regarded as limiting the scope of the present invention. If specific techniques or conditions are not indicated in the examples, the procedures shall be carried out in accordance with the techniques or conditions described in the literature in the field or in accordance with the product specification. The reagents or instruments used without the manufacturer's indication are all conventional products that can be purchased. Main reagents:

[0031] 1) Bovine Serum Albumin (BSA), American Sigma Company;

[0032] 2) Williams E medium, Life Technology;

[0033] 3) DMEM high sugar medium, Hyclone;

[0034] 4) Dimethyl Sulfoxide (DMSO), Sigma Company;

[0035] 5) Fetal bovine serum, BI company;

[0036] 6) 4-ethylpiperazine ethanesulfonic acid (HE...

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Abstract

The invention relates to a tree shrew hepatocyte immortalized cell line and its construction method and application and belongs to the technical field of biomedicine. The cell line is named as ITH6.1, is preserved in the China center for type culture collection in the Wuhan University at No. 229, Bayi road, Wuchang District, Wuhan City, Hubei Province and has a preservation number CCTCC NO: C201740. After continuous passage 100 times, the tree shrew hepatocyte immortalized cell line ITH6.1 still keeps a diploid karyotype and has a very strong multiplication capacity so that immortalization is realized really. The cell line has a strong multiplication capacity and realizes protein expression and biochemical index characteristic analysis of ITH6.1. The result shows that the ITH6.1 has typical tree shrew hepatocyte characteristics. The safety test such as a tumorigenicity test also indicates that the cell line has better biosecurity.

Description

Technical field [0001] The invention belongs to the field of biomedicine technology, and specifically relates to a tree shrew hepatocyte immortalized cell line and its construction method and application. Background technique [0002] Tree shrew (Tupaia belangeri) is a small animal of the mammalian climbing shrew that lives in tropical and subtropical regions. It resembles a squirrel and is a close relative of primates. Because tree shrews have the advantages of small size, easy availability, easy domestication, strong reproductive capacity, low feeding and management costs, and susceptibility to human viruses, they are often used to replace or reduce the use of some non-human primates, so it is very early It is used as an animal model for diseases of primates (including humans). In recent years, studies have found that it has been widely used in the research of hepatitis C, hepatitis B, hand, foot and mouth disease, liver cancer, diabetes, tumors and other diseases. [0003] Liv...

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Application Information

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IPC IPC(8): C12N5/10C12N15/867
CPCC12N5/067C12N15/86C12N2510/04C12N2740/15043C12N2800/107
Inventor 马娜董少忠寸韡
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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